纖維介素蛋白(FGL2)檢測試劑盒DIY材料(酶聯免疫吸附試驗法)
ELISA Kit DIY Materials for Fibrinogen Like Protein 2 (FGL2)
T49; PT49; Fibroleukin
- 編號KSA512Mu01
- 物種Mus musculus (Mouse,小鼠) 相同的名稱,不同的物種。
- 試劑內容 包被抗體、檢測抗體、標準品、HRP標記的鏈霉親和素、TMB底物、96孔板
- 可檢測樣本Plasma.
- 可適用原理雙抗夾心酶聯免疫吸附試驗法測抗原
- 可檢測范圍0.312-20ng/mL
- 可適靈敏度0.137ng/mL
- 應用Main materials for "Do It (ELISA Kit) Yourself".
- 下載 英文說明書 中文說明書
- 規格 96T*596T*10 96T*20 96T*50 96T*100
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特異性
試劑盒中的抗體對纖維介素蛋白(FGL2)具有高靈敏度和高特異性,經檢測與其它相似物質無明顯交叉反應。
用法
1.用100μL每孔的工作濃度的包被抗體包被96孔板,4度孵育過夜或者37度孵育2小時;
2.洗板1次;
3.用200μL每孔的工作濃度的封閉液,37度孵育1.5小時;
4.洗板1次。酶標板即可用于ELISA實驗,后續使用方法與ELISA同。
儲存
抗體,標準品和HRP標記的鏈霉親和素在-20度保存,TMB需在4度保存,96孔板在室溫保存。所有組分保質期為1年,試劑一旦開封,在4度保存時間為1個月。
配套試劑
增值服務
相關產品
編號 | 適用物種:Mus musculus (Mouse,小鼠) | 應用(僅供研究使用,不用于臨床診斷!) |
RPA512Mu02 | 纖維介素蛋白(FGL2)重組蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
RPA512Mu01 | 纖維介素蛋白(FGL2)重組蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
PAA512Mu02 | 纖維介素蛋白(FGL2)多克隆抗體 | WB; IHC; ICC; IP. |
PAA512Mu01 | 纖維介素蛋白(FGL2)多克隆抗體 | WB; IHC; ICC; IP. |
SEA512Mu | 纖維介素蛋白(FGL2)檢測試劑盒(酶聯免疫吸附試驗法) | Enzyme-linked immunosorbent assay for Antigen Detection. |
LMA512Mu | 纖維介素蛋白(FGL2)等多因子檢測試劑盒(流式熒光發光法) | FLIA Kit for Antigen Detection. |
KSA512Mu01 | 纖維介素蛋白(FGL2)檢測試劑盒DIY材料(酶聯免疫吸附試驗法) | Main materials for "Do It (ELISA Kit) Yourself". |
參考文獻
雜志 | 參考文獻 |
Gut. | Expression of B and T lymphocyte attenuator (BTLA) in macrophages contributes to the fulminant hepatitis caused by murine hepatitis virus strain-3 [PubMed: 22637698] |
Hepatogastroenterology. | Plasma fibrinogen-like protein 2 levels in patients with non-alcoholic fatty liver disease. [PubMed: 22024080] |
PLoS Pathogens | Programmed Death (PD)-1-Deficient Mice Are Extremely Sensitive to Murine Hepatitis Virus Strain-3 (MHV-3) Infection [PubMed: PMC3131267] |
Immunology letters | Tumor necrosis factor α (TNF-α) receptor-I is required for TNF-α-mediated fulminant virus hepatitis caused by murine hepatitis virus strain-3 infection. [Pubmed: 24286726 ] |
Immunol Lett. | Tumor necrosis factor α (TNF-α) receptor-I is required for TNF-α-mediated fulminant virus hepatitis caused by murine hepatitis virus strain-3 infection [Pubmed:24286726] |
NATURE COMMUNICATIONS | VSIG4 inhibits proinflammatory macrophage activation by reprogramming mitochondrial pyruvate metabolism [pubmed:29109438] |
Cancer?Medicine | Soluble fibrinogen‐like protein 2 ameliorates acute rejection of liver transplantation in rat via inducing Kupffer cells M2 polarization [Pubmed:29749104] |
BMC?Infectious?Diseases | Soluble fibrinogen-like protein 2 levels in patients with hepatitis B virus-related liver diseases [Pubmed: 30419833] |
Int J Gynaecol Obstet | Tandem mass tag?\based quantitative proteomic profiling of the serum of patients with abnormal uterine bleeding associated with copper intrauterine device [33354778] |
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