70kDa熱休克蛋白1A(HSPA1A)檢測試劑盒DIY材料(酶聯免疫吸附試驗法)
ELISA Kit DIY Materials for Heat Shock 70kDa Protein 1A (HSPA1A)
HSP70-1; HSP-A1A; HSPA1; HSP70-2; HSX70
- 編號KSB081Ra01
- 物種Rattus norvegicus (Rat，大鼠) 相同的名稱，不同的物種。
- 試劑內容 包被抗體、檢測抗體、標準品、HRP標記的鏈霉親和素、TMB底物、96孔板
- 可檢測樣本Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
- 可適用原理雙抗夾心酶聯免疫吸附試驗法測抗原
- 可檢測范圍1.56-100ng/mL
- 可適靈敏度0.59ng/mL
- 應用Main materials for "Do It (ELISA Kit) Yourself".
- 下載 英文說明書 中文說明書
- 規格 96T*596T*10 96T*20 96T*50 96T*100
- 價格 ¥ 7798 ¥ 12996 ¥ 23393 ¥ 45486 ¥ 77976
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產品包裝(模擬)
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產品包裝(模擬)
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通過ISO 9001、ISO 13485質量體系認證
特異性
試劑盒中的抗體對70kDa熱休克蛋白1A(HSPA1A)具有高靈敏度和高特異性，經檢測與其它相似物質無明顯交叉反應。
用法
1.用100μL每孔的工作濃度的包被抗體包被96孔板，4度孵育過夜或者37度孵育2小時；
2.洗板1次；
3.用200μL每孔的工作濃度的封閉液,37度孵育1.5小時；
4.洗板1次。酶標板即可用于ELISA實驗，后續使用方法與ELISA同。
儲存
抗體，標準品和HRP標記的鏈霉親和素在-20度保存，TMB需在4度保存，96孔板在室溫保存。所有組分保質期為1年，試劑一旦開封，在4度保存時間為1個月。
配套試劑
增值服務
相關產品
| 編號 | 適用物種：Rattus norvegicus (Rat，大鼠) | 應用(僅供研究使用，不用于臨床診斷！) |
| RPB081Ra01 | 70kDa熱休克蛋白1A(HSPA1A)重組蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| PAB081Ra01 | 70kDa熱休克蛋白1A(HSPA1A)多克隆抗體 | WB; IHC; ICC; IP. |
| MAB081Ra21 | 70kDa熱休克蛋白1A(HSPA1A)單克隆抗體 | WB; IHC; ICC; IP. |
| SEB081Ra | 70kDa熱休克蛋白1A(HSPA1A)檢測試劑盒(酶聯免疫吸附試驗法) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| LMB081Ra | 70kDa熱休克蛋白1A(HSPA1A)等多因子檢測試劑盒(流式熒光發光法) | FLIA Kit for Antigen Detection. |
| KSB081Ra01 | 70kDa熱休克蛋白1A(HSPA1A)檢測試劑盒DIY材料(酶聯免疫吸附試驗法) | Main materials for "Do It (ELISA Kit) Yourself". |
參考文獻
| 雜志 | 參考文獻 |
| Journal of Neuroinflammation | Identification of potential biomarkers of gold nanoparticle toxicity in rat brains [PubMed: 22691312] |
| Mediators Inflamm.? | Local Inflammation in Fracture Hematoma: Results from a Combined Trauma Model in Pigs [Pubmed:Pmc4324980] |
| Eur J Pharmacol. | Differential modulatory effects of morphine on acute and chronic stress induced neurobehavioral and cellular markers in rats [Pubmed:24508522] |
| Pharmacol Rep. | Effect of propranolol on IL-10, visfatin, Hsp70, iNOS, TLR2, and survivin in amelioration of tumor progression and survival in Solid Ehrlich Carcinoma-bearing mice [Pubmed:25443743] |
| Advances in Medical Sciences | A similar pro/anti-inflammatory cytokine balance is present in the airways of competitive athletes and non-exercising asthmatics [pubmed:28822267] |
| Archives of Medical Research | Winter ambient training conditions are associated with increased bronchial hyperreactivity and with shifts in serum innate immunity proteins in young?… [Pubmed:29379533] |
| metabolic brain disease | Curcumin attenuates cytoplasmic/endoplasmic reticulum stress, apoptosis and cholinergic dysfunction in diabetic rat hippocampus [Pubmed: 32172517] |
| EXPERIMENTAL CELL RESEARCH | Antagomirs targeting miR-142–5p attenuate pilocarpine-induced status epilepticus in mice [Pubmed: 32439493] |
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