甘露糖結(jié)合蛋白(MBL)檢測(cè)試劑盒DIY材料(酶聯(lián)免疫吸附試驗(yàn)法)
ELISA Kit DIY Materials for Mannose Binding Lectin (MBL)
MBL2; COLEC1; HSMBPC; MBP1; MBP; Collectin-1; Mannose-binding protein C; Mannan Binding Protein; Mannose-Binding Lectin(Protein C)2,Soluble(Opsonic Defect)
- 編號(hào)KSB480Ra01
- 物種Rattus norvegicus (Rat，大鼠) 相同的名稱，不同的物種。
- 試劑內(nèi)容 包被抗體、檢測(cè)抗體、標(biāo)準(zhǔn)品、HRP標(biāo)記的鏈霉親和素、TMB底物、96孔板
- 可檢測(cè)樣本Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids.
- 可適用原理雙抗夾心酶聯(lián)免疫吸附試驗(yàn)法測(cè)抗原
- 可檢測(cè)范圍0.312-20ng/mL
- 可適靈敏度0.126ng/mL
- 應(yīng)用Main materials for "Do It (ELISA Kit) Yourself".
- 下載 英文說(shuō)明書(shū) 中文說(shuō)明書(shū)
- 規(guī)格 96T*596T*10 96T*20 96T*50 96T*100
- 價(jià)格 ¥ 7798 ¥ 12996 ¥ 23393 ¥ 45486 ¥ 77976
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產(chǎn)品包裝(模擬)
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通過(guò)ISO 9001、ISO 13485質(zhì)量體系認(rèn)證
特異性
試劑盒中的抗體對(duì)甘露糖結(jié)合蛋白(MBL)具有高靈敏度和高特異性，經(jīng)檢測(cè)與其它相似物質(zhì)無(wú)明顯交叉反應(yīng)。
用法
1.用100μL每孔的工作濃度的包被抗體包被96孔板，4度孵育過(guò)夜或者37度孵育2小時(shí)；
2.洗板1次；
3.用200μL每孔的工作濃度的封閉液,37度孵育1.5小時(shí)；
4.洗板1次。酶標(biāo)板即可用于ELISA實(shí)驗(yàn)，后續(xù)使用方法與ELISA同。
儲(chǔ)存
抗體，標(biāo)準(zhǔn)品和HRP標(biāo)記的鏈霉親和素在-20度保存，TMB需在4度保存，96孔板在室溫保存。所有組分保質(zhì)期為1年，試劑一旦開(kāi)封，在4度保存時(shí)間為1個(gè)月。
配套試劑
贈(zèng)品
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相關(guān)產(chǎn)品
| 編號(hào) | 適用物種：Rattus norvegicus (Rat，大鼠) | 應(yīng)用(僅供研究使用，不用于臨床診斷！) |
| RPB480Ra02 | 甘露糖結(jié)合蛋白(MBL)重組蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| RPB480Ra01 | 甘露糖結(jié)合蛋白(MBL)重組蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| PAB480Ra02 | 甘露糖結(jié)合蛋白(MBL)多克隆抗體 | WB; IHC; ICC; IP. |
| PAB480Ra01 | 甘露糖結(jié)合蛋白(MBL)多克隆抗體 | WB; IHC; ICC; IP. |
| LAB480Ra71 | 甘露糖結(jié)合蛋白(MBL)多克隆抗體(生物素標(biāo)記) | WB; IHC; ICC. |
| SEB480Ra | 甘露糖結(jié)合蛋白(MBL)檢測(cè)試劑盒(酶聯(lián)免疫吸附試驗(yàn)法) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| LMB480Ra | 甘露糖結(jié)合蛋白(MBL)等多因子檢測(cè)試劑盒(流式熒光發(fā)光法) | FLIA Kit for Antigen Detection. |
| KSB480Ra01 | 甘露糖結(jié)合蛋白(MBL)檢測(cè)試劑盒DIY材料(酶聯(lián)免疫吸附試驗(yàn)法) | Main materials for "Do It (ELISA Kit) Yourself". |
參考文獻(xiàn)
| 雜志 | 參考文獻(xiàn) |
| American Journal of Nephrology | Analysis of the Urine Proteome of Human Contrast-Induced Kidney Injury Using Two-Dimensional Fluorescence Differential Gel Electrophoresis/Matrix-Assisted Laser Desorption Time-of-Flight Mass Spectrometry/Liquid Chromatography Mass Spectrometry. [Karger: 000255439] |
| Current Eye Research | Serum Levels and H/L Gene Polymorphism of Mannose-Binding Lectin in Primary Open Angle Glaucoma [Ingenta: art00007] |
| Journal of Clinical Immunology | Complement activation contributes to the injury and outcome of kidney in human anti-glomerular basement membrane disease. [PubMed: 22941511] |
| Clinical and Experimental Immunology | Urinary mannose-binding lectin is a biomarker for predicting the progression of immunoglobulin (Ig)A nephropathy [PubMed: PMC3406374] |
| Clin J Am Soc Nephrol. | Alternative Complement Pathway Activation Products in Urine and Kidneys of Patients with ANCA-Associated GN [Pubmed: 24115193] |
| ACTA TROPICA | Novel findings on the role of ficolins and colectins in the innate response against Leishmania braziliensis [Pubmed: 32827456] |
| PLoS One | Complement activation profile of patients with primary focal segmental glomerulosclerosis [Pubmed: 32569286] |
| LIFE SCIENCES | The lectin pathway of complement and the initial recognition of Leishmania infantum promastigotes [34242658] |
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