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粘蛋白2(MUC2)檢測試劑盒DIY材料(酶聯免疫吸附試驗法)

ELISA Kit DIY Materials for Mucin 2 (MUC2)

MLP; SMUC; Oligomeric Mucus/Gel-Forming; Mucin 2,Intestinal/Tracheal

  • 粘蛋白2(MUC2)檢測試劑盒DIY材料(酶聯免疫吸附試驗法) 產品包裝(模擬)
  • 粘蛋白2(MUC2)檢測試劑盒DIY材料(酶聯免疫吸附試驗法) 產品包裝(模擬)
  • Certificate 通過ISO 9001、ISO 13485質量體系認證

特異性

試劑盒中的抗體對粘蛋白2(MUC2)具有高靈敏度和高特異性,經檢測與其它相似物質無明顯交叉反應。

用法

1.用100μL每孔的工作濃度的包被抗體包被96孔板,4度孵育過夜或者37度孵育2小時;
2.洗板1次;
3.用200μL每孔的工作濃度的封閉液,37度孵育1.5小時;
4.洗板1次。酶標板即可用于ELISA實驗,后續使用方法與ELISA同。

儲存

抗體,標準品和HRP標記的鏈霉親和素在-20度保存,TMB需在4度保存,96孔板在室溫保存。所有組分保質期為1年,試劑一旦開封,在4度保存時間為1個月。

配套試劑

相關產品

編號 適用物種:Homo sapiens (Human,人) 應用(僅供研究使用,不用于臨床診斷!)
RPA705Hu01 粘蛋白2(MUC2)重組蛋白 Positive Control; Immunogen; SDS-PAGE; WB.
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LAA705Hu71 粘蛋白2(MUC2)多克隆抗體(生物素標記) WB; IHC; ICC.
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MAA705Hu24 粘蛋白2(MUC2)單克隆抗體 WB; IHC; ICC; IP.
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MEA705Hu 粘蛋白2(MUC2)檢測試劑盒(酶聯免疫吸附試驗法,小樣本) Enzyme-linked immunosorbent assay for Antigen Detection.
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KSA705Hu01 粘蛋白2(MUC2)檢測試劑盒DIY材料(酶聯免疫吸附試驗法) Main materials for "Do It (ELISA Kit) Yourself".

參考文獻

雜志 參考文獻
Annals of surgical oncology Pancreatic cyst fluid and serum mucin levels predict dysplasia in intraductal papillary mucinous neoplasms of the pancreas. [PubMed: 20717734]
World J Gastroenterol. Sodium alginate ameliorates indomethacin-induced gastrointestinal mucosal injury via inhibiting translocation in rats. [Pubmed:24627600]
Front Microbiol Fecal microbiota transplantation and bacterial consortium transplantation have comparable effects on the re-establishment of mucosal barrier function in mice with intestinal dysbiosis [PubMed: 26217323]
nutrition research and practice Butyrate modulates bacterial adherence on LS174T human colorectal cells by stimulating mucin secretion and MAPK signaling pathway [PubMed: 26244071]
Respirology Secreted mucins and airway bacterial colonization in non-CF bronchiectasis [PubMed: 26172851]
Ann Am Thorac Soc Airway Mucin 2 Is Decreased in Patients with Severe Chronic Obstructive Pulmonary Disease with Bacterial Colonization [doi:10.1513]
Cellular Microbiology An adherent mucus layer attenuates the genotoxic effect of colibactin. [pubmed:29156489]
Bio-protocol Enhancement of Mucus Production in Eukaryotic Cells and Quantification of Adherent Mucus by ELISA [10.21769:BioProtoc.2879]
Journal of Investigative?Medicine The characteristics and clinical significance of mucin levels in bronchoalveolar lavage fluid of patients with interstitial lung disease [Pubmed: 30573494]
Biomolecules Clinical Significance of Serum Membrane-Bound Mucin-2 Levels in Breast Cancer [Pubmed: 30682816]
Cell Stem Cell PRDM16 Maintains Homeostasis of the Intestinal Epithelium by Controlling Region-Specific Metabolism [Pubmed: 31564549]
DIGESTIVE DISEASES AND SCIENCES Compromised Ileal Mucus Barrier Due to Impaired Epithelial Homeostasis Caused by Notch1 Signaling in Cirrhotic Rats [Pubmed: 32144600]
Scientific Reports Mucus layer modeling of human colonoids during infection with enteroaggragative E. coli [Pubmed: 32601325]
Biorxiv Defective intestinal repair after short-term high fat diet due to loss of efferocytosis []
J Cell Mol Med Inhibition of cyclooxygenase?\2 enhanced intestinal epithelial homeostasis via suppressing |??\catenin signalling pathway in experimental liver fibrosis [34145945]
Cell Tissue Res Reduced acetylcholine and elevated muscarinic receptor 2 in duodenal mucosa contribute to the impairment of mucus secretion in 6-hydroxydopamine-induced?… [34370080]
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