S100鈣結合蛋白A6(S100A6)檢測試劑盒DIY材料(酶聯免疫吸附試驗法)
ELISA Kit DIY Materials for S100 Calcium Binding Protein A6 (S100A6)
S100-A6; 2A9; 5B10; CABP; CACY; PRA; MLN 4; Calcyclin; Growth factor-inducible protein 2A9; Prolactin receptor-associated protein
- 編號KSB769Ra01
- 物種Rattus norvegicus (Rat,大鼠) 相同的名稱,不同的物種。
- 試劑內容 包被抗體、檢測抗體、標準品、HRP標記的鏈霉親和素、TMB底物、96孔板
- 可檢測樣本Serum, plasma, tissue homogenates and other biological fluids
- 可適用原理雙抗夾心酶聯免疫吸附試驗法測抗原
- 可檢測范圍0.156-10ng/mL
- 可適靈敏度0.062ng/mL
- 應用Main materials for "Do It (ELISA Kit) Yourself".
- 下載 英文說明書 中文說明書
- 規格 96T*596T*10 96T*20 96T*50 96T*100
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特異性
試劑盒中的抗體對S100鈣結合蛋白A6(S100A6)具有高靈敏度和高特異性,經檢測與其它相似物質無明顯交叉反應。
用法
1.用100μL每孔的工作濃度的包被抗體包被96孔板,4度孵育過夜或者37度孵育2小時;
2.洗板1次;
3.用200μL每孔的工作濃度的封閉液,37度孵育1.5小時;
4.洗板1次。酶標板即可用于ELISA實驗,后續使用方法與ELISA同。
儲存
抗體,標準品和HRP標記的鏈霉親和素在-20度保存,TMB需在4度保存,96孔板在室溫保存。所有組分保質期為1年,試劑一旦開封,在4度保存時間為1個月。
配套試劑
增值服務
相關產品
編號 | 適用物種:Rattus norvegicus (Rat,大鼠) | 應用(僅供研究使用,不用于臨床診斷!) |
RPB769Ra01 | S100鈣結合蛋白A6(S100A6)重組蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
PAB769Ra01 | S100鈣結合蛋白A6(S100A6)多克隆抗體 | WB; IHC; ICC; IP. |
LAB769Ra71 | S100鈣結合蛋白A6(S100A6)多克隆抗體(生物素標記) | WB; IHC; ICC. |
MAB769Ra21 | S100鈣結合蛋白A6(S100A6)單克隆抗體 | WB; IHC; ICC; IP. |
SEB769Ra | S100鈣結合蛋白A6(S100A6)檢測試劑盒(酶聯免疫吸附試驗法) | Enzyme-linked immunosorbent assay for Antigen Detection. |
LMB769Ra | S100鈣結合蛋白A6(S100A6)等多因子檢測試劑盒(流式熒光發光法) | FLIA Kit for Antigen Detection. |
KSB769Ra01 | S100鈣結合蛋白A6(S100A6)檢測試劑盒DIY材料(酶聯免疫吸附試驗法) | Main materials for "Do It (ELISA Kit) Yourself". |
參考文獻
雜志 | 參考文獻 |
Journal of Proteomics | Tear proteome and protein network analyses reveal a novel pentamarker panel for tear film characterization in dry eye and meibomian gland dysfunction [PubMed: 23201116] |
Tumour Biol | Diagnostic significance of S100A2 and S100A6 levels in sera of patients with non-small cell lung cancer [PubMed: 26361956] |
Clinical and Translational Medicine | Serum levels of S100A6 are unaltered in patients with resectable cholangiocarcinoma [pubmed:27709523] |
Gastroenterology | Tu1938-Micronutrients Assessment in Patients with Cirrhosis [10.1016:S0016-5085(18)33544-3] |
Gastroenterology | Tu1942-PI3K/AKT-Mediated Upregulation of C7 Inhibits Colorectal Cancer Proliferation and Metastasis [10.1016:S0016-5085(18)33544-5] |
Tumor?Biology | Proteomics detection of S100A6 in tumor tissue interstitial fluid and evaluation of its potential as a biomarker of cholangiocarcinoma [Pubmed:29629840] |
scandinavian journal of clinical & laboratory investigation | Serum S100A6, S100A8, S100A9 and S100A11 proteins in colorectal neoplasia: results of a single centre prospective study [Pubmed: 31856598] |
CELL BIOCHEMISTRY AND FUNCTION | S100A6 represses Calu?\6 lung cancer cells growth via inhibiting cell proliferation, migration, invasion and enhancing apoptosis [34008212] |
MOLECULAR VISION | Quantification of a panel for dry-eye protein biomarkers in tears: A comparative pilot study using standard ELISA and customized microarrays [34012227] |
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