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S100鈣結(jié)合蛋白A9(S100A9)檢測(cè)試劑盒DIY材料(酶聯(lián)免疫吸附試驗(yàn)法)

ELISA Kit DIY Materials for S100 Calcium Binding Protein A9 (S100A9)

MRP14; MIF; 60B8AG; CFAG; NIF; CAGB; CGLB; L1AG; P14; Calgranulin B; Migration inhibitory factor-related protein 14; Calprotectin L1H subunit; Leukocyte L1 complex heavy chain

  • S100鈣結(jié)合蛋白A9(S100A9)檢測(cè)試劑盒DIY材料(酶聯(lián)免疫吸附試驗(yàn)法) 產(chǎn)品包裝(模擬)
  • S100鈣結(jié)合蛋白A9(S100A9)檢測(cè)試劑盒DIY材料(酶聯(lián)免疫吸附試驗(yàn)法) 產(chǎn)品包裝(模擬)
  • Certificate 通過ISO 9001、ISO 13485質(zhì)量體系認(rèn)證

特異性

試劑盒中的抗體對(duì)S100鈣結(jié)合蛋白A9(S100A9)具有高靈敏度和高特異性,經(jīng)檢測(cè)與其它相似物質(zhì)無明顯交叉反應(yīng)。

用法

1.用100μL每孔的工作濃度的包被抗體包被96孔板,4度孵育過夜或者37度孵育2小時(shí);
2.洗板1次;
3.用200μL每孔的工作濃度的封閉液,37度孵育1.5小時(shí);
4.洗板1次。酶標(biāo)板即可用于ELISA實(shí)驗(yàn),后續(xù)使用方法與ELISA同。

儲(chǔ)存

抗體,標(biāo)準(zhǔn)品和HRP標(biāo)記的鏈霉親和素在-20度保存,TMB需在4度保存,96孔板在室溫保存。所有組分保質(zhì)期為1年,試劑一旦開封,在4度保存時(shí)間為1個(gè)月。

配套試劑

相關(guān)產(chǎn)品

編號(hào) 適用物種:Homo sapiens (Human,人) 應(yīng)用(僅供研究使用,不用于臨床診斷!)
APB793Hu01 S100鈣結(jié)合蛋白A9(S100A9)活性蛋白 Cell?culture;?Activity?Assays.
RPB793Hu01 S100鈣結(jié)合蛋白A9(S100A9)重組蛋白 Positive Control; Immunogen; SDS-PAGE; WB.
PAB793Hu01 S100鈣結(jié)合蛋白A9(S100A9)多克隆抗體 WB; IHC; ICC; IP.
LAB793Hu81 S100鈣結(jié)合蛋白A9(S100A9)多克隆抗體(異硫氰酸熒光素標(biāo)記) WB; IHC; ICC; IF.
LAB793Hu71 S100鈣結(jié)合蛋白A9(S100A9)多克隆抗體(生物素標(biāo)記) WB; IHC; ICC.
MAB793Hu29 S100鈣結(jié)合蛋白A9(S100A9)單克隆抗體 WB; IHC; ICC; IP.
MAB793Hu22 S100鈣結(jié)合蛋白A9(S100A9)單克隆抗體 WB; IHC
MAB793Hu21 S100鈣結(jié)合蛋白A9(S100A9)單克隆抗體 WB; IHC
MAB793Hu23 S100鈣結(jié)合蛋白A9(S100A9)單克隆抗體 WB
MAB793Hu27 S100鈣結(jié)合蛋白A9(S100A9)單克隆抗體 WB; IHC; ICC; IP.
MAB793Hu24 S100鈣結(jié)合蛋白A9(S100A9)單克隆抗體 WB; IHC
MAB793Hu25 S100鈣結(jié)合蛋白A9(S100A9)單克隆抗體 WB; IHC; ICC; IP.
LAB793Hu82 S100鈣結(jié)合蛋白A9(S100A9)單克隆抗體(異硫氰酸熒光素標(biāo)記) WB; IHC; ICC; IF.
LAB793Hu72 S100鈣結(jié)合蛋白A9(S100A9)單克隆抗體(生物素標(biāo)記) WB; IHC; ICC.
SEB793Hu S100鈣結(jié)合蛋白A9(S100A9)檢測(cè)試劑盒(酶聯(lián)免疫吸附試驗(yàn)法) Enzyme-linked immunosorbent assay for Antigen Detection.
LMB793Hu S100鈣結(jié)合蛋白A9(S100A9)等多因子檢測(cè)試劑盒(流式熒光發(fā)光法) FLIA Kit for Antigen Detection.
KSB793Hu01 S100鈣結(jié)合蛋白A9(S100A9)檢測(cè)試劑盒DIY材料(酶聯(lián)免疫吸附試驗(yàn)法) Main materials for "Do It (ELISA Kit) Yourself".

參考文獻(xiàn)

雜志 參考文獻(xiàn)
J. Proteome Res. Differential Proteomic Analysis Distinguishes Tissue Repair Biomarker Signatures in Wound Exudates Obtained from Normal Healing and Chronic Wounds [Pubmed: source]
Biochemical and Biophysical Research Communications High circulating levels of S100A8/A9 complex (calprotectin) in male Japanese with abdominal adiposity and dysregulated expression of S100A8 and S100A9 in adipose tissues of obese mice [ScienceDirect: S0006291X1200352X]
PLoS One. Proteins with Altered Levels in Plasma from Glioblastoma Patients as Revealed by iTRAQ-Based Quantitative Proteomic Analysis [PubMed: 23029420]
Mainz Identifizierung neuer potentieller Biomarker für das Kolonkarzinom sowie dessen Vorstufen [Info:Source]
PLOS ONE Susceptibility to COPD: Differential Proteomic Profiling after Acute Smoking [Pubmed:25036363]
the faseb journal Soluble receptor for advanced glycation end products inhibits disease progression in autosomal dominant polycystic kidney disease by down-regulating cell proliferation [PubMed: 25934702]
Sci Rep.? S100A9 and ORM1 serve as predictors of therapeutic response and prognostic factors in advanced extranodal NK/T cell lymphoma patients treated with pegaspargase/gemcitabine [pmc:PMC4810364]
Journal?of?Alzheimer's?Disease Proteomics?Analysis?of?Blood?Serums?from?Alzheimer's?Disease?Patients?Using?iTRAQ?LabelingTechnology. [pubmed:27911324]
International journal of molecular sciences Immune Modulating Topical S100A8/A9 Inhibits Growth of Pseudomonas aeruginosa and Mitigates Biofilm Infection in Chronic Wounds. [pubmed:28672877]
Gastroenterology Tu1938-Micronutrients Assessment in Patients with Cirrhosis [10.1016:S0016-5085(18)33544-3]
Gastroenterology Tu1942-PI3K/AKT-Mediated Upregulation of C7 Inhibits Colorectal Cancer Proliferation and Metastasis [10.1016:S0016-5085(18)33544-5]
Journal of Biological Chemistry Signaling between pancreatic β-cells and macrophages via S100 calcium-binding protein A8 exacerbates β-cell apoptosis and islet inflammation [Pubmed:29496993]
Cancer?Management and?Research The role of calgranulin B gene on the biological behavior of squamous cervical cancer in vitro and in vivo [Pubmed:29497331]
European?review for?medical?and pharmacological?sciences S100A8/A9 promotes MMP-9 expression in the fibroblasts from cardiac rupture after myocardial infarction by inducing macrophages secreting TNFα. [Pubmed:29949169]
Estudio de células inmunosupresoras y biomarcadores circulantes en carcinoma renal []
Lung Mass Spectrometry Analysis of the Exhaled Breath Condensate and Proposal of Dermcidin and S100A9 as Possible Markers for Lung Cancer Prognosis [Pubmed: 31115649]
中國醫(yī)藥導(dǎo)報(bào) 降鈣素原, 超敏C 反應(yīng)蛋白, 紅細(xì)胞沉降率及S100A9 對(duì)肺結(jié)核合并肺部細(xì)菌感染的影響 []
scandinavian journal of clinical & laboratory investigation Serum S100A6, S100A8, S100A9 and S100A11 proteins in colorectal neoplasia: results of a single centre prospective study [Pubmed: 31856598]
PLoS One Adverse reproductive effects of S100A9 on bovine sperm and early embryonic development in vitro [Pubmed: 31945120]
Sci Rep Overexpression of S100A9 in tumor stroma contribute to immune evasion of NK/T cell lymphoma and predict poor response rate [34045609]
university of copenhagen Adjunctive S100A8/A9 Immunomodulation Hinders Cipro fl oxacin Resistance in []
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