維生素D結(jié)合蛋白(DBP)檢測(cè)試劑盒DIY材料(酶聯(lián)免疫吸附試驗(yàn)法)
ELISA Kit DIY Materials for Vitamin D Binding Protein (DBP)
GC; VDBG; VDBP; Group-Specific Component; Gc-globulin
- 編號(hào)KSB810Ra01
- 物種Rattus norvegicus (Rat，大鼠) 相同的名稱(chēng)，不同的物種。
- 試劑內(nèi)容 包被抗體、檢測(cè)抗體、標(biāo)準(zhǔn)品、HRP標(biāo)記的鏈霉親和素、TMB底物、96孔板
- 可檢測(cè)樣本Serum, plasma and other biological fluids.
- 可適用原理雙抗夾心酶聯(lián)免疫吸附試驗(yàn)法測(cè)抗原
- 可檢測(cè)范圍3.12-200ng/mL
- 可適靈敏度1.42ng/mL
- 應(yīng)用Main materials for "Do It (ELISA Kit) Yourself".
- 下載 英文說(shuō)明書(shū) 中文說(shuō)明書(shū)
- 規(guī)格 96T*596T*10 96T*20 96T*50 96T*100
- 價(jià)格 ¥ 7798 ¥ 12996 ¥ 23393 ¥ 45486 ¥ 77976
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通過(guò)ISO 9001、ISO 13485質(zhì)量體系認(rèn)證
特異性
試劑盒中的抗體對(duì)維生素D結(jié)合蛋白(DBP)具有高靈敏度和高特異性，經(jīng)檢測(cè)與其它相似物質(zhì)無(wú)明顯交叉反應(yīng)。
用法
1.用100μL每孔的工作濃度的包被抗體包被96孔板，4度孵育過(guò)夜或者37度孵育2小時(shí)；
2.洗板1次；
3.用200μL每孔的工作濃度的封閉液,37度孵育1.5小時(shí)；
4.洗板1次。酶標(biāo)板即可用于ELISA實(shí)驗(yàn)，后續(xù)使用方法與ELISA同。
儲(chǔ)存
抗體，標(biāo)準(zhǔn)品和HRP標(biāo)記的鏈霉親和素在-20度保存，TMB需在4度保存，96孔板在室溫保存。所有組分保質(zhì)期為1年，試劑一旦開(kāi)封，在4度保存時(shí)間為1個(gè)月。
配套試劑
贈(zèng)品
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相關(guān)產(chǎn)品
| 編號(hào) | 適用物種：Rattus norvegicus (Rat，大鼠) | 應(yīng)用(僅供研究使用，不用于臨床診斷！) |
| RPB810Ra02 | 維生素D結(jié)合蛋白(DBP)重組蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| RPB810Ra01 | 維生素D結(jié)合蛋白(DBP)重組蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| PAB810Ra02 | 維生素D結(jié)合蛋白(DBP)多克隆抗體 | WB; IHC; ICC; IP. |
| PAB810Ra01 | 維生素D結(jié)合蛋白(DBP)多克隆抗體 | WB; IHC; ICC; IP. |
| LAB810Ra71 | 維生素D結(jié)合蛋白(DBP)多克隆抗體(生物素標(biāo)記) | WB; IHC; ICC. |
| MAB810Ra24 | 維生素D結(jié)合蛋白(DBP)單克隆抗體 | WB; IHC; ICC; IP. |
| MAB810Ra22 | 維生素D結(jié)合蛋白(DBP)單克隆抗體 | WB; IHC; ICC; IP. |
| MAB810Ra21 | 維生素D結(jié)合蛋白(DBP)單克隆抗體 | WB; IHC; ICC; IP. |
| MAB810Ra23 | 維生素D結(jié)合蛋白(DBP)單克隆抗體 | WB; IHC; ICC; IP. |
| FAB810Ra02 | 抗維生素D結(jié)合蛋白(DBP)單克隆抗體 | Flow cytometry. |
| FAB810Ra04 | 抗維生素D結(jié)合蛋白(DBP)單克隆抗體 | Flow cytometry. |
| FAB810Ra06 | 抗維生素D結(jié)合蛋白(DBP)單克隆抗體 | Flow cytometry. |
| FAB810Ra08 | 抗維生素D結(jié)合蛋白(DBP)單克隆抗體 | Flow cytometry. |
| SEB810Ra | 維生素D結(jié)合蛋白(DBP)檢測(cè)試劑盒(酶聯(lián)免疫吸附試驗(yàn)法) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| LMB810Ra | 維生素D結(jié)合蛋白(DBP)等多因子檢測(cè)試劑盒(流式熒光發(fā)光法) | FLIA Kit for Antigen Detection. |
| KSB810Ra01 | 維生素D結(jié)合蛋白(DBP)檢測(cè)試劑盒DIY材料(酶聯(lián)免疫吸附試驗(yàn)法) | Main materials for "Do It (ELISA Kit) Yourself". |
參考文獻(xiàn)
| 雜志 | 參考文獻(xiàn) |
| Proteome Science | Isotope Coded Protein Labeling analysis of plasma specimens from acute severe dengue fever patients [PubMed: 23101585] |
| Toxins (Basel). | Increased Circulating Levels of Vitamin D Binding Protein in MS Patients [Pubmed:25590278] |
| Endocrine | Vitamin D-binding protein and free vitamin D concentrations in acromegaly [PubMed: 26547217] |
| Proteomics Clinical Applications | Comparative mass spectrometric and immunoassay‐based proteome analysis in serum of Duchenne muscular dystrophy patients [Pubmed:26680509] |
| PLoS One. | 25-Hydroxivitamin D Serum Concentration, Not Free and Bioavailable Vitamin D, Is Associated with Disease Activity in Systemic Lupus Erythematosus Patients. [pubmed:28085957] |
| PROTEOMICS?-?Clinical?Applications | Comparative mass spectrometric and immunoassay-based?proteome?analysis?in?serum?ofDuchenne muscular dystrophy?patients. [pubmed:26680509] |
| Plos One | 25-Hydroxivitamin D serum concentration, not free and bioavailable vitamin D, is associated with disease activity in systemic lupus erythematosus patients [10.1371/journal.pone.0170323] |
| Nutrients | Gene Expression of Sirtuin-1 and Endogenous Secretory Receptor for Advanced Glycation End Products in Healthy and Slightly Overweight Subjects after?… [Pubmed:30037068] |
| BBA- Molecular Basis of Disease | Enhanced remedial effects for vitamin D3 and calcium co-supplementation against pre-existing lead nephrotoxicity in mice: The roles of renal calcium homeostatic?… [Pubmed: 30553018] |
| J Clin Res Pediatr Endocrinol | Is Bioavailable Vitamin D Better Than Total Vitamin D to Evaluate Vitamin D Status in Obese Children? [34013709] |
| PLoS One | Leukocyte telomere length as a compensatory mechanism in vitamin D metabolism [Pubmed:35202418] |
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