非組織特異性堿性磷酸酶(ALPL)活性蛋白
Active Alkaline Phosphatase, Tissue-nonspecific (ALPL)
BALP; BSAP; HOPS; AP-TNAP; TNSALP; Bone Alkaline Phosphatase; Alkaline Phosphatase,Tissue-Nonspecific Isozyme; Alkaline Phosphatase, Liver/Bone/Kidney
- 編號APB091Hu61
- 物種Homo sapiens (Human，人) 相同的名稱，不同的物種。
- 緩沖液成份PBS, pH7.4, containing 5% Trehalose.
- 性狀凍干粉
- 純度> 90%
- 等電點6.7
- 應(yīng)用Cell?culture;?Activity?Assays.
- 下載 英文說明書 中文說明書
- 規(guī)格 10μg50μg 200μg 1mg 5mg
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產(chǎn)品包裝(模擬)
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產(chǎn)品包裝(模擬)
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Figure. SDS-PAGE
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通過ISO 9001、ISO 13485質(zhì)量體系認(rèn)證
活性實驗
Four distinct genes encode alkaline phosphatases (APs) in humans. The ALPL gene encodes the liver/bone/kidney isozyme, also known as the tissue nonspecific AP (TNAP). In comparison, ALPI, ALPP and ALPPL2 encode intestinal, placental and placental-like or germ cell APs, respectively. The serum levels of human APs are useful tumor markers. There are many mutations in the ALPL gene, leading to different forms of hypophosphatasia, characterized by poorly mineralized cartilage and bones. The native ALPL is a glycosylated homodimer attached to the membrane through a GPI-anchor. The activity assay of recombinant human ALPL was measured by its ability to cleave a peptide substrate, 4-Methumbelliferly phosphate. The reaction was performed in 50 mM Tris, 1 mM MgCl2, pH 9.0 ( assay buffer), ainitiated by addition 50 μL of 0.625 ug/ml ALPL (diluted by assay buffer) to 50 μL of 50 uM substrate. Read at excitation and emission wavelengths of 365 nm and 445 nm (top read), respectively, in kinetic mode for 5 minutes. The specific activity of recombinant human ALPL is >11000 pmol/min/μg.
用法
Reconstitute in 10mM PBS (pH7.4) to a concentration of 0.1-1.0 mg/mL. Do not vortex.
儲存
避免反復(fù)凍融。2-8°C不超過一個月，-80°C不超過12個月。
穩(wěn)定性
熱穩(wěn)定性以損失率顯示。損失率是由加速降解試驗決定，具體方法如下：在37°C孵育48小時，沒有顯著的降解或者沉淀產(chǎn)生。保質(zhì)期內(nèi)，在適當(dāng)?shù)臈l件下存儲，損失率低于5%。
增值服務(wù)
相關(guān)產(chǎn)品
| 編號 | 適用物種：Homo sapiens (Human，人) | 應(yīng)用(僅供研究使用，不用于臨床診斷！) |
| RPB091Hu01 | 非組織特異性堿性磷酸酶(ALPL)重組蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| APB091Hu61 | 非組織特異性堿性磷酸酶(ALPL)活性蛋白 | Cell?culture;?Activity?Assays. |
| EPB091Hu61 | 非組織特異性堿性磷酸酶(ALPL)真核蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| PAB091Hu01 | 非組織特異性堿性磷酸酶(ALPL)多克隆抗體 | WB; IHC; ICC; IP. |
| MAB091Hu23 | 非組織特異性堿性磷酸酶(ALPL)單克隆抗體 | WB; IHC; ICC; IP. |
| MAB091Hu21 | 非組織特異性堿性磷酸酶(ALPL)單克隆抗體 | WB; IHC; ICC; IP. |
| MAB091Hu24 | 非組織特異性堿性磷酸酶(ALPL)單克隆抗體 | WB; IHC; ICC; IP. |
| MAB091Hu22 | 非組織特異性堿性磷酸酶(ALPL)單克隆抗體 | WB,IHC |
| SEB091Hu | 非組織特異性堿性磷酸酶(ALPL)檢測試劑盒(酶聯(lián)免疫吸附試驗法) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| LMB091Hu | 非組織特異性堿性磷酸酶(ALPL)等多因子檢測試劑盒(流式熒光發(fā)光法) | FLIA Kit for Antigen Detection. |
參考文獻(xiàn)
| 雜志 | 參考文獻(xiàn) |
| Journal of Materials Science: Materials in Medicine | Osteopenic bone cell response to strontium-substituted hydroxyapatite [SpringerLink: n8233337w171v451] |
| Osteoporosis International | Dextromethorphan inhibits osteoclast differentiation by suppressing RANKL-induced nuclear factor-κB activation [PubMed: 23400250] |
| Journal of Applied Toxicology | Chronic exposure to low concentrations of strontium 90 affects bone physiology but not the hematopoietic system in mice [Wiley: Source] |
| Acta Pharmacologica Sinica | The antidepressant bupropion exerts alleviating properties in an ovariectomized osteoporotic rat model [Pubmed:25544359] |
| Biomedical Materials | Enhanced differentiation of osteoblastic cells on novel chitosan/β-1, 3-glucan/bioceramic scaffolds for bone tissue regeneration [Pubmed:25586067] |
| Pharmacology | Effect of Mirtazapine on Rat Bone Tissue after Orchidectomy [PubMed: 25871861] |
| J Biomed Mater Res A. | New method for the fabrication of highly osteoconductive β-1,3-glucan/HA scaffold for bone tissue engineering: Structural, mechanical, and biological characterization. [Pubmed:27239050] |
| J Bone Miner Metab | MiR-142-5p promotes bone repair by maintaining osteoblast activity [Pubmed:27085967] |
| Journal of Biomedical Materials Research | New method for the fabrication of highly osteoconductive β‐1, 3‐glucan/HA scaffold for bone tissue engineering: Structural, mechanical, and biological … [doi:10.1002] |
| Journal of?Bone?and?Mineral?Metabolism | MiR?142?5p promotes bone repair by maintaining osteoblast activity [pubmed:27085967] |
| Scientific Reports | Hydrolysis of Extracellular Pyrophosphate increases in post-hemodialysis plasma [Pubmed:30038263] |
| International?Journal?of?Molecular?Sciences? | Suppression Effect of Astaxanthin on Osteoclast Formation In Vitro and Bone Loss In Vivo [Pubmed:29562730] |
| Pharmacology | Effects of Amlodipine on Bone Metabolism in Orchidectomised Spontaneously Hypertensive Rats [Pubmed:29898457] |
| Experimental and Therapeutic Medicine | Combination therapy with BMP?2 and psoralen enhances fracture healing in ovariectomized mice [10.3892:etm.2018.6353] |
| American Journal of Translational?Research | Long non-coding RNA SNHG7 promotes the fracture repair through negative modulation of miR-9 [] |
| The effect of low temperature atmospheric nitrogen plasma on MC3T3-E1 preosteoblast proliferation and differentiation in vitro [] | |
| International Journal of Biomaterials | Bovine Hydroxyapatite-Based Bone Scaffold with Gentamicin Accelerates Vascularization and Remodeling of Bone Defect [34104195] |
