含銅胺氧化酶3(AOC3)活性蛋白
Active Amine Oxidase, Copper Containing 3 (AOC3)
VAP1; HPAO; SSAO; Membrane primary amine oxidase; Vascular Adhesion Protein 1; Semicarbazide-Sensitive Amine Oxidase; Copper-Containing Amine Oxygen Oxidoreductase
- 編號APC298Hu61
- 物種Homo sapiens (Human，人) 相同的名稱，不同的物種。
- 緩沖液成份PBS, pH7.4, containing 5% Trehalose.
- 性狀凍干粉
- 純度> 90%
- 等電點6.4
- 應用Cell?culture;?Activity?Assays.
- 下載 英文說明書 中文說明書
- 規格 10μg50μg 200μg 1mg 5mg
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產品包裝(模擬)
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產品包裝(模擬)
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Figure. SDS-PAGE
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通過ISO 9001、ISO 13485質量體系認證
活性實驗
Amine Oxidase, Copper Containing 3 (AOC3) is a copper amine oxidase with a topaquinone cofactor. AOC3 is a Type II integral membrane protein, but a soluble form of the enzyme is present in human serum, and its level increases in diabetes and some inflammatory liver diseases. AOC3 catalyzes the oxidative deamination of small primary amines such as methylamine, benzylamine, and aminoacetone in a reaction that produces an aldehyde, ammonia, and H2O2. The enzyme is sensitive to inhibition by semicarbazide. AOC3 expression is highest in the endothelium of lung, heart, and intestine, but low in tissues such as brain, spleen, kidney, and liver. The activity of recombinant human AOC3 was measured by its ability to produce hydrogen peroxide during the oxidation of benzylamine. The reaction was performed in 50 mM HEPES, pH 7.5 (assay buffer), initiated by addition 50 μL of various concentrations of AOC3 (diluted by assay buffer) to 50 μl substrate mixture of 2 mM Benzylamine, 2 units/mL HRP and 100 μM AUR. Read at excitation and emission wavelengths of 544 nm and 590 nm (top read), respectively in kinetic mode for 5 minutes.
用法
Reconstitute in 10mM PBS (pH7.4) to a concentration of 0.1-1.0 mg/mL. Do not vortex.
儲存
避免反復凍融。2-8°C不超過一個月，-80°C不超過12個月。
穩定性
熱穩定性以損失率顯示。損失率是由加速降解試驗決定，具體方法如下：在37°C孵育48小時，沒有顯著的降解或者沉淀產生。保質期內，在適當的條件下存儲，損失率低于5%。
增值服務
相關產品
| 編號 | 適用物種：Homo sapiens (Human，人) | 應用(僅供研究使用，不用于臨床診斷！) |
| APC298Hu61 | 含銅胺氧化酶3(AOC3)活性蛋白 | Cell?culture;?Activity?Assays. |
| EPC298Hu61 | 含銅胺氧化酶3(AOC3)真核蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
