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膜聯蛋白A1(ANXA1)活性蛋白

Active Annexin A1 (ANXA1)

ANX-A1; ANX1; LPC1; Lipocortin I; Chromobindin-9; Calpactin II; Phospholipase A2 inhibitory protein

  • 膜聯蛋白A1(ANXA1)活性蛋白 產品包裝(模擬)
  • 膜聯蛋白A1(ANXA1)活性蛋白 產品包裝(模擬)
  • 膜聯蛋白A1(ANXA1)活性蛋白 Gene sequencing
  • APE787Hu01.png SDS-PAGE圖
  • 膜聯蛋白A1(ANXA1)活性蛋白 Figure. Western Blot
  • Certificate 通過ISO 9001、ISO 13485質量體系認證

活性實驗

Annexin A1 (ANXA1), also known as lipocortin I, belongs to the annexin family of Ca2 -dependent phospholipid-binding proteins that have a molecular weight of approximately 35,000 to 40,000 and are preferentially located on the cytosolic face of the plasma membrane. Annexin A1 protein has an apparent relative molecular mass of 40kDa with phospholipase A2 inhibitory activity. Besides, S100 Calcium Binding Protein A11 (S100A11) has been identified as an interactor of ANXA1, thus a binding ELISA assay was conducted to detect the interaction of recombinant human ANXA1 and recombinant human S100A11. Briefly, ANXA1 were diluted serially in PBS, with 0.01% BSA (pH 7.4). Duplicate samples of 100μL were then transferred to S100A11-coated microtiter wells and incubated for 2h at 37℃. Wells were washed with PBST and incubated for 1h with anti-ANXA1 pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody, wells were aspirated and washed 3 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37℃. Finally, add 50μL stop solution to the wells and read at 450nm immediately. The binding activity of ANXA1 and S100A11 was shown in Figure 1, and this effect was in a dose dependent manner.
Figure. The binding activity of ANXA1 with S100A11.

用法

Reconstitute in 20mM Tris, 150mM NaCl (pH8.0) to a concentration of 0.1-1.0 mg/mL. Do not vortex.

儲存

避免反復凍融。2-8°C不超過一個月,-80°C不超過12個月。

穩定性

熱穩定性以損失率顯示。損失率是由加速降解試驗決定,具體方法如下:在37°C孵育48小時,沒有顯著的降解或者沉淀產生。保質期內,在適當的條件下存儲,損失率低于5%。

相關產品

編號 適用物種:Homo sapiens (Human,人) 應用(僅供研究使用,不用于臨床診斷!)
APE787Hu01 膜聯蛋白A1(ANXA1)活性蛋白 Cell?culture;?Activity?Assays.
RPE787Hu01 膜聯蛋白A1(ANXA1)重組蛋白 Positive Control; Immunogen; SDS-PAGE; WB.
PAE787Hu01 膜聯蛋白A1(ANXA1)多克隆抗體 WB; IHC; ICC; IP.
PAE787Hu02 膜聯蛋白A1(ANXA1)多克隆抗體 WB; IHC; ICC; IP.
LAE787Hu81 膜聯蛋白A1(ANXA1)多克隆抗體(異硫氰酸熒光素標記) WB; IHC; ICC; IF.
LAE787Hu71 膜聯蛋白A1(ANXA1)多克隆抗體(生物素標記) WB; IHC; ICC.
MAE787Hu22 膜聯蛋白A1(ANXA1)單克隆抗體 WB
MAE787Hu21 膜聯蛋白A1(ANXA1)單克隆抗體 WB; IHC; ICC; IP.
SEE787Hu 膜聯蛋白A1(ANXA1)檢測試劑盒(酶聯免疫吸附試驗法) Enzyme-linked immunosorbent assay for Antigen Detection.
AEE787Hu 抗膜聯蛋白A1抗體(Anti-ANXA1)檢測試劑盒(酶聯免疫吸附試驗法) Enzyme-linked immunosorbent assay for Antibody Detection.
SCE787Hu 膜聯蛋白A1(ANXA1)檢測試劑盒(化學發光免疫分析法) Chemiluminescent immunoassay for Antigen Detection.
LME787Hu 膜聯蛋白A1(ANXA1)等多因子檢測試劑盒(流式熒光發光法) FLIA Kit for Antigen Detection.
KSE787Hu01 膜聯蛋白A1(ANXA1)檢測試劑盒DIY材料(酶聯免疫吸附試驗法) Main materials for "Do It (ELISA Kit) Yourself".

參考文獻

雜志 參考文獻
Plos one Dysregulation of Anti-Inflammatory Annexin A1 Expression in Progressive Crohns Disease [PubMed: PMC3794972]
African Journals Online Modulatory Effect of Phytoestrogens and Curcumin on Induction of Annexin 1 in Human Peripheral Blood Mononuclear Cells and their Inhibitory Effect on Secretory Phospholipase A2 [Ajol: Source]
Antimicrobial Agents and Chemotherapy Host Biomarkers of Invasive Pulmonary Aspergillosis to Monitor Therapeutic Response [Asm: Source]
J Proteomics Characterization of secretomes from a human blood brain barrier endothelial cells in-vitro model after ischemia by stable isotope labeling with aminoacids in cell culture (SILAC) [PubMed: 26718731]
Diabetes Annexin A1 Is a Key Modulator of Mesenchymal Stromal Cell–Mediated Improvements in Islet Function [PubMed: 26470781]
PLoS One Annexin A1 Is Increased in the Plasma of Preeclamptic Women [PubMed: 26398190]
中國現代醫學雜志 China Journal of Modern Medicine 膜聯蛋白A1 和CA19-9 在膽管癌中的表達及其臨床意義 [article:8686]
arteriosclerosis thrombosis and vascular biology Protective Aptitude of Annexin A1 in Arterial Neointima Formation in Atherosclerosis-Prone Mice-Brief Report. [pubmed:28062503]
Clinical Science Mesenchymal stromal cells improve human islet function through released products and extracellular matrix. [pubmed:29101297]
Biomedicine & Pharmacotherapy Proresolving protein Annexin A1: The role in type 2 diabetes mellitus and obesity [Pubmed:29677533]
?International Journal of?Chronic?Obstructive Pulmonary Disease Annexin A1 is elevated in patients with COPD and affects lung fibroblast function [Pubmed:29440885]
Medical?Mycology Applying host disease status biomarkers to therapeutic response monitoring in invasive aspergillosis patients [Pubmed:29370415]
DEVELOPMENTAL NEUROPSYCHOLOGY EFFECTS OF MULTICOMPONENT EXERCISE ON PROGRESSION OF BRAIN ATROPHY AND COGNITION IN AMNESTIC MILD COGNITIVE IMPAIRMENT?… [pdf:3395]
Multiple sclerosis and related disorders Resolution of inflammation in neuromyelitis optica spectrum disorders [Pubmed: 30300851]
Acta Pharmaceutica Effects of annexin A1 on apoptosis and cell cycle arrest in human leukemic cell lines []
npj Science of Food Annexin A1 accounts for an anti-inflammatory binding target of sesamin metabolites [Pubmed: 32133417]
FASEB JOURNAL ANXA1 directs Schwann cells proliferation and migration to accelerate nerve regeneration through the FPR2/AMPK pathway [Pubmed: 32908567]
Rev Soc Bras Med Trop Expression of annexin-A1 in blood and tissue leukocytes of leprosy patients [33263684]
science advances Annexin-A1 SUMOylation regulates microglial polarization after cerebral ischemia by modulating IKK|á stability via selective autophagy [33523920]
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