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骨成型蛋白4(BMP4)活性蛋白

Active Bone Morphogenetic Protein 4 (BMP4)

BMP2B; BMP2-B; BMP2B1; ZYME; DVR4; Bone morphogenetic protein 2B

  • 骨成型蛋白4(BMP4)活性蛋白 產(chǎn)品包裝(模擬)
  • 骨成型蛋白4(BMP4)活性蛋白 產(chǎn)品包裝(模擬)
  • APA014Bo01.jpg Figure. SDS-PAGE
  • Certificate 通過ISO 9001、ISO 13485質(zhì)量體系認(rèn)證

活性實(shí)驗(yàn)

BMP4 (Bone morphogenetic protein 4) is a member of the bone morphogenetic protein family, which is involved in bone and cartilage development, specifically tooth and limb development and fracture repair. To test the effect of BMP4 on cell apoptosis, HepG2 cells were seeded into triplicate wells of 96-well plates at a density of 4,000 cells/well and allowed to attach overnight, then the medium was replaced with various concentrations of recombinant bovine BMP4 diluted with 5% serum standard DMEM. After incubated for 72h,?cells were observed by inverted microscope and cell proliferation was measured by Cell Counting Kit-8 (CCK-8). Briefly, 10 μl of CCK-8 solution was added to each well of the plate, then the absorbance at 450 nm was measured using a microplate reader after incubating the plate for 1-4 hours at 37 ℃. Apoptosis of HepG2 cells after incubation with BMP4 for 72h observed by inverted microscope was shown in Figure 1. Cell viability was assessed by CCK-8 (Cell Counting Kit-8 ) assay after incubation with recombinant bovine BMP4 for 72h. The result was shown in Figure 2. It was obvious that BMP4 significantly decreased cell viability of HepG2 cells. The ED50 of recombinant bovine BMP4 is 3.07 ug/ml.

Figure 2. Inhibition of HepG2 cells proliferation after stimulated with recombinant bovine BMP4

用法

Reconstitute in 10mM PBS (pH7.4) to a concentration of 0.1-1.0 mg/mL. Do not vortex.

儲(chǔ)存

避免反復(fù)凍融。2-8°C不超過一個(gè)月,-80°C不超過12個(gè)月。

穩(wěn)定性

熱穩(wěn)定性以損失率顯示。損失率是由加速降解試驗(yàn)決定,具體方法如下:在37°C孵育48小時(shí),沒有顯著的降解或者沉淀產(chǎn)生。保質(zhì)期內(nèi),在適當(dāng)?shù)臈l件下存儲(chǔ),損失率低于5%。

相關(guān)產(chǎn)品

編號(hào) 適用物種:Bos taurus; Bovine (Cattle,牛) 應(yīng)用(僅供研究使用,不用于臨床診斷!)
RPA014Bo01 骨成型蛋白4(BMP4)重組蛋白 Positive Control; Immunogen; SDS-PAGE; WB.
APA014Bo01 骨成型蛋白4(BMP4)活性蛋白 Cell?culture;?Activity?Assays.
PAA014Bo01 骨成型蛋白4(BMP4)多克隆抗體 WB; IHC; ICC; IP.
LAA014Bo71 骨成型蛋白4(BMP4)多克隆抗體(生物素標(biāo)記) WB; IHC; ICC.
MAA014Bo21 骨成型蛋白4(BMP4)單克隆抗體 WB; IHC; ICC; IP.
SEA014Bo 骨成型蛋白4(BMP4)檢測(cè)試劑盒(酶聯(lián)免疫吸附試驗(yàn)法) Enzyme-linked immunosorbent assay for Antigen Detection.
SCA014Bo 骨成型蛋白4(BMP4)檢測(cè)試劑盒(化學(xué)發(fā)光免疫分析法) Chemiluminescent immunoassay for Antigen Detection.
LMA014Bo 骨成型蛋白4(BMP4)等多因子檢測(cè)試劑盒(流式熒光發(fā)光法) FLIA Kit for Antigen Detection.

參考文獻(xiàn)

雜志 參考文獻(xiàn)
Journal of Cellular Biochemistry Titanium with Nanotopography Induces Osteoblast Differentiation by Regulating Endogenous Bone Morphogenetic Protein Expression and Signaling Pathway [PubMed: 26681207]
Domestic Animal Endocrinology Expression analysis of bone morphogenetic protein 4 between fat and lean birds in adipose tissue and serum [Pubmed:26945137]
FEBS?Open?Bio Bone morphogenetic protein 4 (BMP 4) regulates immortalized chicken preadipocyte proliferation by promoting G1/S cell cycle progression [Pubmed: 30972973]
Arch Gynecol Obstet The relationship between transforming growth factor β superfamily members (GDF11 and BMP4) and lumbar spine bone mineral density in postmenopausal Chinese?… [34417839]
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