碳酸酐酶Ⅱ(CA2)活性蛋白
Active Carbonic Anhydrase II (CA2)
CA-II; CAII; Car2; CAC; Carbonate dehydratase II; Carbonic anhydrase C
- 編號APA782Ra01
- 物種Rattus norvegicus (Rat，大鼠) 相同的名稱，不同的物種。
- 緩沖液成份20mM Tris, 150mM NaCl緩沖液（pH8.0, 含有1mM EDTA, 1mM DTT, 0.01% SKL, 5% Trehalose和Proclin300）
- 性狀凍干粉
- 純度> 97%
- 等電點6.9
- 應用Cell?culture;?Activity?Assays.
- 下載 英文說明書 中文說明書
- 規格 10μg50μg 200μg 1mg 5mg
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產品包裝(模擬)
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產品包裝(模擬)
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SDS-PAGE圖
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通過ISO 9001、ISO 13485質量體系認證
活性實驗
CA2 (Carbonic anhydrase 2) is an enzyme that catalyzes reversible hydration of carbon dioxide. It is essential for bone resorption and osteoclast differentiation and contributes to intracellular pH regulation in the duodenal upper villous epithelium during proton-coupled peptide absorption. It is widely accepted that CA2 also catalyzes hydrolysis of p-Nitrophenyl Acetate. Thus, a hydration assay was conducted to test the catalytic activity of CA2 using 4-Nitrophenyl Acetate (4-NPA) as substrate. Briefly, different concentrations of CA2 were incubated with 1mM 4-NPA in reaction buffer. The absorbance at the wavelength of 400nm was read per hour, and the result was shown in figure 1. It is obvious that CA2 catalyzes hydrolysis of p-Nitrophenyl Acetate.
用法
Reconstitute in 20mM Tris, 150mM NaCl (pH8.0) to a concentration of 0.1-1.0 mg/mL. Do not vortex.
儲存
避免反復凍融。2-8°C不超過一個月，-80°C不超過12個月。
穩定性
熱穩定性以損失率顯示。損失率是由加速降解試驗決定，具體方法如下：在37°C孵育48小時，沒有顯著的降解或者沉淀產生。保質期內，在適當的條件下存儲，損失率低于5%。
增值服務
相關產品
| 編號 | 適用物種：Rattus norvegicus (Rat，大鼠) | 應用(僅供研究使用，不用于臨床診斷！) |
| NPA782Ra01 | 碳酸酐酶Ⅱ(CA2)天然蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| APA782Ra02 | 碳酸酐酶Ⅱ(CA2)活性蛋白 | Cell?culture;?Activity?Assays. |
| APA782Ra01 | 碳酸酐酶Ⅱ(CA2)活性蛋白 | Cell?culture;?Activity?Assays. |
| RPA782Ra01 | 碳酸酐酶Ⅱ(CA2)重組蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| PAA782Ra02 | 碳酸酐酶Ⅱ(CA2)多克隆抗體 | WB; IHC; ICC; IP. |
| PAA782Ra01 | 碳酸酐酶Ⅱ(CA2)多克隆抗體 | WB; IHC; ICC; IP. |
| LAA782Ra71 | 碳酸酐酶Ⅱ(CA2)多克隆抗體(生物素標記) | WB; IHC; ICC. |
| SEA782Ra | 碳酸酐酶Ⅱ(CA2)檢測試劑盒(酶聯免疫吸附試驗法) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| LMA782Ra | 碳酸酐酶Ⅱ(CA2)等多因子檢測試劑盒(流式熒光發光法) | FLIA Kit for Antigen Detection. |
| KSA782Ra01 | 碳酸酐酶Ⅱ(CA2)檢測試劑盒DIY材料(酶聯免疫吸附試驗法) | Main materials for "Do It (ELISA Kit) Yourself". |
參考文獻
| 雜志 | 參考文獻 |
| Proteomics Clin Appl.? | Identification of potential breast cancer markers in nipple discharge by protein profile analysis using two-dimensional nano-liquid chromatography/nanoelectrospray ionization-mass spectrometry. [Pubmed:26970563] |
| PROTEOMICS?-?Clinical?Applications | Comparative mass spectrometric and immunoassay-based?proteome?analysis?in?serum?ofDuchenne muscular dystrophy?patients. [pubmed:26680509] |
| Cell & Molecular Biology | Identification of potential breast cancer markers in nipple discharge by protein profile analysis using two‐dimensional nano‐liquid chromatography/nanoelectrospray … [doi:10.1002] |
