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CD146分子(CD146)活性蛋白

Active Cluster Of Differentiation 146 (CD146)

MCAM; MUC18; Melanoma Cell Adhesion Molecule; Cell surface glycoprotein P1H12; Melanoma-associated antigen A32; S-endo 1 endothelial-associated antigen

  • CD146分子(CD146)活性蛋白 產品包裝(模擬)
  • CD146分子(CD146)活性蛋白 產品包裝(模擬)
  • APB670Hu01.jpg Figure. SDS-PAGE
  • Certificate 通過ISO 9001、ISO 13485質量體系認證

活性實驗

CD146?(cluster?of?differentiation?146), also known as MUC18 or MCAM, is a putative adhesion molecule that belongs to the immunoglobulin superfamily (IgSF). It is an approximately 113-kDa type1 transmembrane glycoprotein that?is?expressed?at the intercellular junction of endothelial cells. CD146 was originally identified as a tumor marker for melanoma (MCAM) due to its existence only in melanoma but not in the corresponding normal counterpart. However CD146 is not just a CAM for the inter-cellular and cell-matrix adhesion. Recent evidence indicates that CD146 is actively involved in miscellaneous processes, such as development, signaling transduction, cell migration, mesenchymal stem cells differentiation, angiogenesis and immune response. CD146? and PECAM-1 are both members of immunoglobulin superfamily of cell adhesion molecules. PECAM-1 has been identified as an interactor of CD146, thus a functional binding ELISA assay was conducted to detect the interaction of recombinant human CD146 and recombinant rat PECAM-1. Briefly, CD146 was diluted serially in PBS with 0.01% BSA (pH 7.4). Duplicate samples of 100 ul were then transferred to PECAM-1-coated microtiter wells and incubated for 1h at 37℃. Wells were washed with PBST and incubated for 1h with anti-CD146 pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody for 1h at 37℃, wells were aspirated and washed 5 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37℃. Finally, add 50 ul stop solution to the wells and read at 450/630 nm immediately. The binding activity of recombinant human CD146 and recombinant rat PECAM-1 was shown in Figure 1, the EC50?for this effect is 3.6 ug/mL.

用法

Reconstitute in 10mM PBS (pH7.4) to a concentration of 0.1-1.0 mg/mL. Do not vortex.

儲存

避免反復凍融。2-8°C不超過一個月,-80°C不超過12個月。

穩定性

熱穩定性以損失率顯示。損失率是由加速降解試驗決定,具體方法如下:在37°C孵育48小時,沒有顯著的降解或者沉淀產生。保質期內,在適當的條件下存儲,損失率低于5%。

相關產品

編號 適用物種:Homo sapiens (Human,人) 應用(僅供研究使用,不用于臨床診斷!)
APB670Hu01 CD146分子(CD146)活性蛋白 Cell?culture;?Activity?Assays.
RPB670Hu01 CD146分子(CD146)重組蛋白 Positive Control; Immunogen; SDS-PAGE; WB.
PAB670Hu01 CD146分子(CD146)多克隆抗體 WB; ICC/IF
FAB670Hu01 抗CD146分子(CD146)多克隆抗體 FCM
FAB670Hu81 抗CD146分子(CD146)多克隆抗體(異硫氰酸熒光素標記) FCM
RAB670Hu21 CD146分子(CD146)重組抗體 WB; IF; ICC; IHC; IP; FCM.
MAB670Hu22 CD146分子(CD146)單克隆抗體 WB; IHC; ICC; IP.
SEB670Hu CD146分子(CD146)檢測試劑盒(酶聯免疫吸附試驗法) Enzyme-linked immunosorbent assay for Antigen Detection.
LMB670Hu CD146分子(CD146)等多因子檢測試劑盒(流式熒光發光法) FLIA Kit for Antigen Detection.

參考文獻

雜志 參考文獻
Clinical Rheumatology Evaluation of various endothelial biomarkers in ankylosing spondylitis [PubMed: 21556780]
Inflammation The Effect of Regular Colchicine Treatment on Biomarkers Related with Vascular Injury in Newly Diagnosed Patients with Familial Mediterranean Fever [SpringerLink: m7128503g576w808]
Oncotarget Selective targeting of liver cancer with the endothelial marker CD146 [Pubmed:25238265]
Future Medicine Melanoma cell adhesion molecule as an emerging biomarker with prognostic significance in systolic heart failure [pubmed:27355366]
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