表皮生長因子(EGF)活性蛋白
Active Epidermal Growth Factor (EGF)
URG; Beta-Urogastrone; Pro-epidermal growth factor
- 編號APA560Hu02
- 物種Homo sapiens (Human，人) 相同的名稱，不同的物種。
- 緩沖液成份20mM Tris, 150mM NaCl緩沖液（pH8.0, 含有1mM EDTA, 1mM DTT, 0.01% SKL, 5% Trehalose和Proclin300）
- 性狀凍干粉
- 純度> 90%
- 等電點4.8
- 應用Cell?culture;?Activity?Assays.
- 下載 英文說明書 中文說明書
- 規(guī)格 10μg50μg 200μg 1mg 5mg
- 價格 ¥ 768 ¥ 1920 ¥ 3840 ¥ 11520 ¥ 28800
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產品包裝(模擬)
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產品包裝(模擬)
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Figure. Gene Sequencing (Extract)
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Figure. SDS-PAGE
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Figure. Western Blot; Sample: Recombinant EGF, Human.
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通過ISO 9001、ISO 13485質量體系認證
活性實驗
Epidermal growth factor?(EGF) is a?growth factor?that stimulates?cell growth,?proliferation, and?differentiation?by binding to its receptor?EGFR. To test the effect of EGF on cell proliferation of 3T3 fibroblasts, 3T3 cells were seeded into triplicate wells of 96-well plates at a density of 2, 000cells/well and allowed to attach overnight, then the medium was replaced with serum-free standard DMEM?prior to the addition of various concentrations of EGF. After incubated for 72h,?cells were observed by inverted microscope and cell proliferation was measured by Cell Counting Kit-8 (CCK-8). Briefly, 10μL of CCK-8 solution was added to each well of the plate, then measure the absorbance at 450nm using a microplate reader after incubating the plate for 1-4 hours at 37°C .
Cell proliferation of 3T3 cells after incubation with EGF for 72h observed by inverted microscope was shown in Figure 1.
The dose-effect curve of EGF was shown in Figure 2. It was obvious that EGF significantly promoted cell proliferation of 3T3 cells. The ED50 for this effect is typically 0.9848 to 2.958ng/mL.
用法
Reconstitute in 20mM Tris, 150mM NaCl (pH8.0) to a concentration of 0.1-1.0 mg/mL. Do not vortex.
儲存
避免反復凍融。2-8°C不超過一個月，-80°C不超過12個月。
穩(wěn)定性
熱穩(wěn)定性以損失率顯示。損失率是由加速降解試驗決定，具體方法如下：在37°C孵育48小時，沒有顯著的降解或者沉淀產生。保質期內，在適當的條件下存儲，損失率低于5%。
增值服務
相關產品
| 編號 | 適用物種：Homo sapiens (Human，人) | 應用(僅供研究使用，不用于臨床診斷！) |
| APA560Hu02 | 表皮生長因子(EGF)活性蛋白 | Cell?culture;?Activity?Assays. |
| APA560Hu06 | 表皮生長因子(EGF)活性蛋白 | Cell?culture;?Activity?Assays. |
| RPA560Hu03 | 表皮生長因子(EGF)重組蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| APA560Hu04 | 表皮生長因子(EGF)活性蛋白 | Cell?culture;?Activity?Assays. |
| RPA560Hu08 | 表皮生長因子(EGF)重組蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| RPA560Hu09 | 表皮生長因子(EGF)重組蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| RPA560Hu02 | 表皮生長因子(EGF)重組蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| RPA560Hu06 | 表皮生長因子(EGF)重組蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| RPA560Hu01 | 表皮生長因子(EGF)重組蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| APA560Hu01 | 表皮生長因子(EGF)活性蛋白 | Cell?culture;?Activity?Assays. |
| PAA560Hu06 | 表皮生長因子(EGF)多克隆抗體 | WB; IHC; ICC; IP. |
| PAA560Hu05 | 表皮生長因子(EGF)多克隆抗體 | WB; IHC; ICC; IP. |
| PAA560Hu01 | 表皮生長因子(EGF)多克隆抗體 | WB; IHC; ICC; IP. |
| PAA560Hu02 | 表皮生長因子(EGF)多克隆抗體 | WB; IHC; ICC; IP. |
| LAA560Hu71 | 表皮生長因子(EGF)多克隆抗體(生物素標記) | WB; IHC; ICC. |
| MAA560Hu22 | 表皮生長因子(EGF)單克隆抗體 | WB; IHC |
| MAA560Hu24 | 表皮生長因子(EGF)單克隆抗體 | WB; IHC; ICC; IP. |
| MAA560Hu23 | 表皮生長因子(EGF)單克隆抗體 | WB; IHC; ICC; IP. |
| MAA560Hu25 | 表皮生長因子(EGF)單克隆抗體 | WB; IHC; ICC; IP. |
| MAA560Hu27 | 表皮生長因子(EGF)單克隆抗體 | WB; IHC; ICC; IP. |
| MAA560Hu21 | 表皮生長因子(EGF)單克隆抗體 | WB; IHC; ICC; IP. |
| MAA560Hu29 | 表皮生長因子(EGF)單克隆抗體 | WB; IHC; ICC; IP. |
| MAA560Hu26 | 表皮生長因子(EGF)單克隆抗體 | WB; IHC; ICC; IP. |
| MAA560Hu28 | 表皮生長因子(EGF)單克隆抗體 | WB; IHC; ICC; IP. |
| HEA560Hu | 表皮生長因子(EGF)檢測試劑盒(酶聯(lián)免疫吸附試驗法,高敏型) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| SEA560Hu | 表皮生長因子(EGF)檢測試劑盒(酶聯(lián)免疫吸附試驗法) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| WEA560Hu | 表皮生長因子(EGF)檢測試劑盒(酶聯(lián)免疫吸附試驗法,寬范圍) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| SCA560Hu | 表皮生長因子(EGF)檢測試劑盒(化學發(fā)光免疫分析法) | Chemiluminescent immunoassay for Antigen Detection. |
| LMA560Hu | 表皮生長因子(EGF)等多因子檢測試劑盒(流式熒光發(fā)光法) | FLIA Kit for Antigen Detection. |
| PSA560Hu01 | 表皮生長因子(EGF)抗體對 | ELISA; CLIA; ELISPOT; Luminex; Immunochromatography and other Immunoassays. |
| KSA560Hu01 | 表皮生長因子(EGF)檢測試劑盒DIY材料(酶聯(lián)免疫吸附試驗法) | Main materials for "Do It (ELISA Kit) Yourself". |
參考文獻
| 雜志 | 參考文獻 |
| Journal of Pediatric Surgery | The influence of nutrients, biliary-pancreatic secretions, and systemic trophic hormones on intestinal adaptation in a Roux-en-Y bypass model [PubMed: 20438940] |
| Biomark Insights | Decreased Epidermal Growth Factor (EGF) Associated with HMGB1 and Increased Hyperactivity in Children with Autism [PubMed: PMC3623607] |
| Plos one | The Probiotic Mixture VSL#3 Accelerates Gastric Ulcer Healing by Stimulating Vascular Endothelial Growth Factor [PubMed: PMC3590171] |
| Diabetes. | Allogeneic transplantation of an adipose-derived stem cell (ASC) sheet combined with artificial skin accelerates wound healing in a rat wound model of type 2 diabetes and obesity [Pubmed:25795216] |
| Eur Rev Med Pharmacol Sci.? | Association of colon adenomas and skin tags: coincidence or coexistence? [Pubmed:24763889] |
| Int J Clin Exp Med. | The effects of self-assembling peptide RADA16 hydrogel on malignant phenotype of human hepatocellular carcinoma cell [PubMed: 26628972] |
| ACS Applied Materials & Interfaces | Polydopamine nanoparticles modulating stimuli-responsive PNIPAM hydrogels with cell/tissue adhesiveness. [pubmed:27709887] |
| Canadian Journal of Physiology and Pharmacology | The effect of midkine on growth factors and oxidative status in an experimental wound model in diabetic and healthy rats [pubmed:28177680] |
| Journal of Controlled Release | A DPP-IV-resistant glucagon-like peptide-2 dimer with enhanced activity against radiation-induced intestinal injury [pubmed:28522195] |
| Scientific Reports | Optimization of Storage Temperature for Retention of Undifferentiated Cell Character of Cultured Human Epidermal Cell Sheets [pubmed:28811665] |
| International Journal of?Nanomedicine | Micro-/nano-topography of selective laser melting titanium enhances adhesion and proliferation and regulates adhesion-related gene expressions of human?… [Pubmed: 30233172] |
| Neoplasia | Patient-derived xenograft (PDX) models of colorectal carcinoma (CRC) as a platform for chemosensitivity and biomarker analysis in personalized medicine [Pubmed: 33212364] |
| Aesthet Surg J | Adipose-Derived Stem Cells Improve the Aging Skin of Nude Mice by Promoting Angiogenesis and Reducing Local Tissue Water [33428732] |
| Cell Death & Disease | Sodium/glucose cotransporter 1-dependent metabolic alterations induce tamoxifen resistance in breast cancer by promoting macrophage M2 polarization [34006822] |
| Stem Cells International | The Paracrine Effect of Adipose-Derived Stem Cells Orchestrates Competition between Different Damaged Dermal Fibroblasts to Repair UVB-Induced Skin??- [33381190] |
| Molecular Biology Reports | Umbilical cord-derived mesenchymal stem cell conditioned medium reverses neuronal oxidative injury by inhibition of TRPM2 activation and the JNK signaling?… [Pubmed:35585377] |
| Biomaterials Advances | Human adipose-derived stem cell-loaded small intestinal submucosa as a bioactive wound dressing for the treatment of diabetic wounds in rats [] |
| Authorea | Syringaresinol attenuates kidney injury by inhibition of pyroptosis via activating the Nrf2 antioxidant pathway in diabetic nephropathy [] |
| Biointerface Research in Applied Chemistry | Beetroot Improves the Therapeutic Efficacy of Bone Marrow-Derived Mesenchymal Stem Cells in Preventing Cisplatin Induced Nephrotoxicity in Male Rats [] |
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