半乳糖凝集素4(GAL4)活性蛋白
Active Galectin 4 (GAL4)
LGALS4; Lactose-Binding Lectin 4; L-36 Lactose-Binding Protein; L36LBP; Lectin,Galactoside-Binding Soluble 4
- 編號APA304Bo01
- 物種Bos taurus; Bovine (Cattle，牛) 相同的名稱，不同的物種。
- 緩沖液成份PBS, pH7.4, containing 0.01% SKL, 5% Trehalose.
- 性狀凍干粉
- 純度> 90%
- 等電點9.4
- 應用Cell?culture;?Activity?Assays.
- 下載 英文說明書 中文說明書
- 規格 10μg50μg 200μg 1mg 5mg
- 價格 ¥ 1728 ¥ 4320 ¥ 8640 ¥ 25920 ¥ 64800
- 欲了解實際交易價格和更多情況，請與當地經銷商聯系!
-
產品包裝(模擬)
-
產品包裝(模擬)
-
Figure. SDS-PAGE
-
通過ISO 9001、ISO 13485質量體系認證
活性實驗
Galectin-4(GAL4) is a protein that in humans is encoded by the LGALS4 gene. The galectins are a family of beta-galactoside-binding proteins implicated in modulating cell-cell and cell-matrix interactions. LGALS4 is an S-type lectin that is strongly underexpressed in colorectal cancer. In this case, we chose rabbit erythrocyte (RaE) to assay its ability of agglutination. A general procedure for hemagglutination assay (or haemagglutination assay; HA) is as follows, two-fold dilute the recombinant cattle GAL4 with 0.9% sodium chloride injection, add 50μL a serial dilution of GAL4 to each well of a U or V-bottom shaped 96-well microtiter plate. The final well serves as a negative control without GAL4, replace with 50μL 0.9% sodium chloride injection. Then add 50μL 1% rabbit erythrocyte to each well and mixed gently. The plate is incubated for 3 hours at room temperature. The results are shown in Figure 1. It was obvious that the minimal effective concentration of GAL4 is 3.1 μg/mL.
Figure 2. The hemagglutination assay of GAL4 in V- bottom shaped 96-well microtiter plate.
用法
Reconstitute in 10mM PBS (pH7.4) to a concentration of 0.1-1.0 mg/mL. Do not vortex.
儲存
避免反復凍融。2-8°C不超過一個月，-80°C不超過12個月。
穩定性
熱穩定性以損失率顯示。損失率是由加速降解試驗決定，具體方法如下：在37°C孵育48小時，沒有顯著的降解或者沉淀產生。保質期內，在適當的條件下存儲，損失率低于5%。
增值服務
相關產品
| 編號 | 適用物種：Bos taurus; Bovine (Cattle，牛) | 應用(僅供研究使用，不用于臨床診斷！) |
| APA304Bo01 | 半乳糖凝集素4(GAL4)活性蛋白 | Cell?culture;?Activity?Assays. |
| RPA304Bo01 | 半乳糖凝集素4(GAL4)重組蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| PAA304Bo01 | 半乳糖凝集素4(GAL4)多克隆抗體 | WB; IHC; ICC; IP. |
| CGA304Bo01 | 半乳糖凝集素4(GAL4)cDNA | PCR、蛋白表達等 |
