高爾基磷酸化蛋白2(GOLPH2)活性蛋白
Active Golgi Phosphoprotein 2 (GOLPH2)
GOLM1; GP73; C9orf155; Golgi Membrane Protein 1; Golgi Protein 73
- 編號APB668Mu01
- 物種Mus musculus (Mouse，小鼠) 相同的名稱，不同的物種。
- 緩沖液成份20mM Tris, 150mM NaCl緩沖液（pH8.0, 含有1mM EDTA, 1mM DTT, 0.01% SKL, 5% Trehalose和Proclin300）
- 性狀凍干粉
- 純度> 97%
- 等電點5.2
- 應用Cell?culture;?Activity?Assays.
- 下載 英文說明書 中文說明書
- 規格 10μg50μg 200μg 1mg 5mg
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產品包裝(模擬)
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產品包裝(模擬)
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SDS-PAGE圖
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Figure. Western Blot; Sample: Recombinant GP73, Mouse.
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通過ISO 9001、ISO 13485質量體系認證
活性實驗
Golgi Protein 73 (GP73) also known as Golgi phosphoprotein 2 or Golgi membrane protein GP73 is a protein that encoded by the GOLM1 gene. The Golgi complex plays a key role in the sorting and modification of proteins exported from the endoplasmic reticulum. It has been observed to be upregulated in response to viral infection. Besides, Dymeclin (DYM) has been identified as an interactor of GP73, thus a binding ELISA assay was conducted to detect the interaction of recombinant mouse GP73 and recombinant human DYM. Briefly, GP73 were diluted serially in PBS, with 0.01% BSA (pH 7.4). Duplicate samples of 100uL were then transferred to DYM-coated microtiter wells and incubated for 2h at 37℃. Wells were washed with PBST and incubated for 1h with anti-GP73 pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody, wells were aspirated and washed 3 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37℃. Finally, add 50μL stop solution to the wells and read at 450nm immediately. The binding activity of of GP73 and DYM was shown in Figure 1, and this effect was in a dose dependent manner.
用法
Reconstitute in 20mM Tris, 150mM NaCl (pH8.0) to a concentration of 0.1-1.0 mg/mL. Do not vortex.
儲存
避免反復凍融。2-8°C不超過一個月，-80°C不超過12個月。
穩定性
熱穩定性以損失率顯示。損失率是由加速降解試驗決定，具體方法如下：在37°C孵育48小時，沒有顯著的降解或者沉淀產生。保質期內，在適當的條件下存儲，損失率低于5%。
增值服務
相關產品
| 編號 | 適用物種：Mus musculus (Mouse，小鼠) | 應用(僅供研究使用，不用于臨床診斷！) |
| RPB668Mu02 | 高爾基磷酸化蛋白2(GOLPH2)重組蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| APB668Mu01 | 高爾基磷酸化蛋白2(GOLPH2)活性蛋白 | Cell?culture;?Activity?Assays. |
| RPB668Mu01 | 高爾基磷酸化蛋白2(GOLPH2)重組蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| PAB668Mu02 | 高爾基磷酸化蛋白2(GOLPH2)多克隆抗體 | WB; IHC; ICC; IP. |
| PAB668Mu01 | 高爾基磷酸化蛋白2(GOLPH2)多克隆抗體 | WB; IHC; ICC; IP; FCM |
| LAB668Mu81 | 高爾基磷酸化蛋白2(GOLPH2)多克隆抗體(異硫氰酸熒光素標記) | WB; IHC; ICC; IF. |
| LAB668Mu71 | 高爾基磷酸化蛋白2(GOLPH2)多克隆抗體(生物素標記) | WB; IHC; ICC. |
| SEB668Mu | 高爾基磷酸化蛋白2(GOLPH2)檢測試劑盒(酶聯免疫吸附試驗法) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| LMB668Mu | 高爾基磷酸化蛋白2(GOLPH2)等多因子檢測試劑盒(流式熒光發光法) | FLIA Kit for Antigen Detection. |
| KSB668Mu01 | 高爾基磷酸化蛋白2(GOLPH2)檢測試劑盒DIY材料(酶聯免疫吸附試驗法) | Main materials for "Do It (ELISA Kit) Yourself". |
參考文獻
| 雜志 | 參考文獻 |
| Molecular & Cellular Proteomics | LAMC2: A promising new pancreatic cancer biomarker identified by proteomic analysis of pancreatic adenocarcinoma tissues [Mcponline: 023507] |
| Afro-Egypt J Infect Endem Dis | Diagnostic and Prognostic Validity of Serum Golgi Protein 73 in Egyptian Patients with Hepatocelluar Carcinoma [Ajied: Ajied_System_Files] |
| Journal of Applied Pharmaceutical Science | Diagnostic role of Golgi protein 73 and IL-17 in Egyptian cirrhotic rather than hepatocellular carcinoma patients. [handle:123456789] |
| Oncology Letters | Serum Golgi protein 73 is a prognostic rather than diagnostic marker in hepatocellular carcinoma [pubmed:29113278] |
| Scientific Reports | The possible role of Dickkopf-1, Golgi protein-73 and Midkine as predictors of hepatocarcinogenesis: a review and an Egyptian study [Pubmed: 32198440] |
| Hepatobiliary & Pancreatic Diseases International | Dynamic expression of hepatic GP73 mRNA and protein and circulating GP73 during hepatocytes malignant transformation [Pubmed: 32171652] |
| Biomolecules | Deoxycholic Acid Upregulates Serum Golgi Protein 73 through Activating NF-|êB Pathway and Destroying Golgi Structure in Liver Disease. Biomolecules 2021, 11??- [33540642] |
| ADVANCED RESEARCHES | Search for Effective Serum Tumor Markers for Early Diagnosis of Hepatocellular Carcinoma Associated with Hepatitis C [] |
