白介素17受體D(IL17RD)活性蛋白
Active Interleukin 17 Receptor D (IL17RD)
SEF; IL17RLM; IL-17RD; IL17R-D; IL17Rhom; Interleukin-17 receptor-like protein
- 編號APD349Hu01
- 物種Homo sapiens (Human，人) 相同的名稱，不同的物種。
- 緩沖液成份20mM Tris, 150mM NaCl緩沖液（pH8.0, 含有1mM EDTA, 1mM DTT, 0.01% SKL, 5% Trehalose和Proclin300）
- 性狀凍干粉
- 純度> 90%
- 等電點8.9
- 應用Cell?culture;?Activity?Assays.
- 下載 英文說明書 中文說明書
- 規(guī)格 10μg50μg 200μg 1mg 5mg
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產品包裝(模擬)
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產品包裝(模擬)
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SDS-PAGE圖
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Figure. Western Blot; Sample: Recombinant IL17RD, Human.
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通過ISO 9001、ISO 13485質量體系認證
活性實驗
IL17RD (interleukin-17 receptor D) is a membrane protein belonging to the IL17R protein family, acting as a component of the interleukin-17 receptor signaling complex. Knowing that the interaction between this protein and IL-17R does not require the interleukin, we have conducted a binding a binding ELISA assay to detect the interaction of recombinant human IL17RD with both recombinant human IL17RA and IL17. Briefly, IL17RD were diluted serially in PBS, with 0.01%BSA (pH 7.4). Duplicate samples of 100uL were then transferred to IL17RA-coated or IL17-coated microtiter wells and incubated for 2h at 37°C. Wells were washed with PBST and incubated for 1h with anti-IL17RA pAb and anti-IL17 pAb separately, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody, wells were aspirated and washed 3 times. With the addition of substrate solution , wells were incubated 15-25 minutes at 37°C. Finally, add 50μL stop solution to the wells and read at 450nm immediately. The binding activity of IL17RD with IL17RA and IL17 was shown in Figure 1 and Figure 2 respectively and this effect was in a dose dependent manner.
用法
Reconstitute in 20mM Tris, 150mM NaCl (pH8.0) to a concentration of 0.1-1.0 mg/mL. Do not vortex.
儲存
避免反復凍融。2-8°C不超過一個月，-80°C不超過12個月。
穩(wěn)定性
熱穩(wěn)定性以損失率顯示。損失率是由加速降解試驗決定，具體方法如下：在37°C孵育48小時，沒有顯著的降解或者沉淀產生。保質期內，在適當?shù)臈l件下存儲，損失率低于5%。
增值服務
相關產品
| 編號 | 適用物種：Homo sapiens (Human，人) | 應用(僅供研究使用，不用于臨床診斷！) |
| APD349Hu01 | 白介素17受體D(IL17RD)活性蛋白 | Cell?culture;?Activity?Assays. |
| RPD349Hu01 | 白介素17受體D(IL17RD)重組蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| PAD349Hu01 | 白介素17受體D(IL17RD)多克隆抗體 | WB; IHC; ICC; IP. |
| FAD349Hu01 | 抗白介素17受體D(IL17RD)多克隆抗體 | Flow cytometry. |
