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白介素28B(IL28B)活性蛋白

Active Interleukin 28B (IL28B)

IFNL3; Interferon,Lambda 3; Cytokine Zcyto22

  • 白介素28B(IL28B)活性蛋白 產品包裝(模擬)
  • 白介素28B(IL28B)活性蛋白 產品包裝(模擬)
  • APC028Hu01.jpg Figure. SDS-PAGE
  • 白介素28B(IL28B)活性蛋白 Figure. Western Blot
  • Certificate 通過ISO 9001、ISO 13485質量體系認證

活性實驗

Figure. The binding activity of IL28B with IL10Rb.
Interleukin 28B (IL28B) is a cytokine distantly related to type I interferons and the IL-10 family. It has function on antiviral, antitumour and immunomodulatory activities. IL28B also plays a critical role in the antiviral host defense, predominantly in the epithelial tissues and acts as a ligand for the heterodimeric class II cytokine receptor composed of IL10RB and IFNLR1, and receptor engagement leads to the activation of the JAK/STAT signaling pathway resulting in the expression of IFN-stimulated genes (ISG), which mediate the antiviral state. Besides, Interleukin 10 Receptor Beta (IL10Rb) has been identified as an interactor of IL28B, thus a binding ELISA assay was conducted to detect the interaction of recombinant human IL28B and recombinant human IL10Rb. Briefly, IL28B were diluted serially in PBS, with 0.01% BSA (pH 7.4). Duplicate samples of 100uL were then transferred to IL10Rb-coated microtiter wells and incubated for 2h at 37℃. Wells were washed with PBST and incubated for 1h with anti-IL28B pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody, wells were aspirated and washed 3 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37℃. Finally, add 50μL stop solution to the wells and read at 450nm immediately. The binding activity of IL28B and IL10Rb was shown in Figure 1, and this effect was in a dose dependent manner.

用法

Reconstitute in 20mM Tris, 150mM NaCl (PH8.0) to a concentration of 0.1-1.0 mg/mL. Do not vortex.

儲存

避免反復凍融。2-8°C不超過一個月,-80°C不超過12個月。

穩定性

熱穩定性以損失率顯示。損失率是由加速降解試驗決定,具體方法如下:在37°C孵育48小時,沒有顯著的降解或者沉淀產生。保質期內,在適當的條件下存儲,損失率低于5%。

相關產品

編號 適用物種:Homo sapiens (Human,人) 應用(僅供研究使用,不用于臨床診斷!)
APC028Hu01 白介素28B(IL28B)活性蛋白 Cell?culture;?Activity?Assays.
RPC028Hu01 白介素28B(IL28B)重組蛋白 Positive Control; Immunogen; SDS-PAGE; WB.
PAC028Hu01 白介素28B(IL28B)多克隆抗體 WB; IHC; ICC; IP.
LAC028Hu81 白介素28B(IL28B)多克隆抗體(異硫氰酸熒光素標記) WB; IHC; ICC; IF.
LAC028Hu71 白介素28B(IL28B)多克隆抗體(生物素標記) WB; IHC; ICC.
MAC028Hu22 白介素28B(IL28B)單克隆抗體 WB; IHC; ICC; IP.
HEC028Hu 白介素28B(IL28B)檢測試劑盒(酶聯免疫吸附試驗法,高敏型) Enzyme-linked immunosorbent assay for Antigen Detection.
SEC028Hu 白介素28B(IL28B)檢測試劑盒(酶聯免疫吸附試驗法) Enzyme-linked immunosorbent assay for Antigen Detection.
SCC028Hu 白介素28B(IL28B)檢測試劑盒(化學發光免疫分析法) Chemiluminescent immunoassay for Antigen Detection.
LMC028Hu 白介素28B(IL28B)等多因子檢測試劑盒(流式熒光發光法) FLIA Kit for Antigen Detection.

參考文獻

雜志 參考文獻
Journal of Antimicrobial Chemotherapy Impact of IL28B gene polymorphisms on interferon-λ3 plasma levels during pegylated interferon-α/ribavirin therapy for chronic hepatitis C in patients coinfected with HIV [PubMed: 22294646]
Cytokine Different effects of three interferons L on Toll-like receptor-related gene expression in HepG2 cells. [Pubmed: 24041672]
Hepatology Polymorphisms in melanoma differentiation‐associated gene 5 link protein function to clearance of hepatitis C virus [Pubmed:25130193]
Cytokine. Increased concentration of interferon lambda-3, interferon beta and interleukin-10 in the cerebrospinal fluid of patients with tick-borne encephalitis [Pubmed:25461389]
J Biomed Res.? Impact of IL28B gene polymorphisms rs8099917 and rs12980275 on response to pegylated interferon-α/ribavirin therapy in chronic hepatitis C genotype 4 … [pmc:PMC4726832]
Journal of Biomedical Research Impact of?IL28B?gene polymorphisms rs8099917 and rs12980275 on response to pegylated interferon-α/ribavirin therapy in chronic hepatitis C genotype 4 patients [PMC4726832]
LUPUS Collagen triple helix repeat containing-1: a novel biomarker associated with disease activity in Systemic lupus erythematosus [Pubmed: 30336754]
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