白介素6受體(IL6R)活性蛋白
Active Interleukin 6 Receptor (IL6R)
CD126; gp80; IL6R; IL-6R-1; IL-6R-Alpha; IL6RA; IL-6 receptor subunit alpha; Membrane glycoprotein 80
- 編號APB815Hu61
- 物種Homo sapiens (Human,人) 相同的名稱,不同的物種。
- 緩沖液成份PBS, pH7.4, containing 5% Trehalose.
- 性狀凍干粉
- 純度> 95%
- 等電點7.2
- 應用Cell?culture;?Activity?Assays.
- 下載 英文說明書 中文說明書
- 規格 10μg50μg 200μg 1mg 5mg
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活性實驗
![](http://static.cloud-clone.cn/product/pictures/APB815Hu61-202306141428114670.jpg)
Interleukin-6 receptor (IL-6R) is a receptor for IL-6, belonging to the type I cytokine receptor family, subfamily 3. IL-6 is a potent pleiotropic cytokine that regulates cell growth and differentiation and plays an important role in immune response. IL-6R is a protein complex composed of this protein and the interleukin-6 signal transductor (IL6ST/GP130/IL6β). This receptor subunit is also shared by many other cytokines. Dysproduction of IL-6 and this receptor has been implicated in the pathogenesis of many diseases, such as multiple myeloma, autoimmune diseases and prostate cancer. In addition, IL-6Rα (IL-6RA) is the primary functional alpha subunit of the IL-6 receptor, which is also a component of other interleukin receptors. IL-6RA is a type I transmembrane glycoprotein that regulates the biological activity of IL-6 by forming a complex with CD130. IL-6R also plays an important role in acute response and hematopoietic response. A binding ELISA assay was conducted to detect the association of recombinant human IL6R with recombinant human JAK2. Briefly, biotin-linked IL6R were diluted serially in PBS, with 0.01% BSA (pH 7.4). Duplicate samples of 100 μl were then transferred to JAK2-coated microtiter wells and incubated for 1h at 37℃. Wells were washed with PBST 3 times and incubation with Streptavidin-HRP for 30min, then wells were aspirated and washed 5 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37℃. Finally, add 50 μl stop solution to the wells and read at 450 nm immediately. The binding activity of IL6R and JAK2 was shown in Figure 1, the EC50?for this effect is 1.182 ug/mL.
用法
Reconstitute in 10mM PBS (pH7.4) to a concentration of 0.1-1.0 mg/mL. Do not vortex.
儲存
避免反復凍融。2-8°C不超過一個月,-80°C不超過12個月。
穩定性
熱穩定性以損失率顯示。損失率是由加速降解試驗決定,具體方法如下:在37°C孵育48小時,沒有顯著的降解或者沉淀產生。保質期內,在適當的條件下存儲,損失率低于5%。
增值服務
相關產品
編號 | 適用物種:Homo sapiens (Human,人) | 應用(僅供研究使用,不用于臨床診斷!) |
APB815Hu61 | 白介素6受體(IL6R)活性蛋白 | Cell?culture;?Activity?Assays. |
EPB815Hu61 | 白介素6受體(IL6R)真核蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
RPB815Hu01 | 白介素6受體(IL6R)重組蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
PAB815Hu01 | 白介素6受體(IL6R)多克隆抗體 | WB; IHC; ICC; IP. |
LAB815Hu71 | 白介素6受體(IL6R)多克隆抗體(生物素標記) | WB; IHC; ICC. |
RAB815Hu21 | 白介素6受體(IL6R)重組抗體 | WB; IF; ICC; IHC; IP; FCM. |
MAB815Hu22 | 白介素6受體(IL6R)單克隆抗體 | WB; IHC; ICC; IP. |
SEB815Hu | 白介素6受體(IL6R)檢測試劑盒(酶聯免疫吸附試驗法) | Enzyme-linked immunosorbent assay for Antigen Detection. |
LMB815Hu | 白介素6受體(IL6R)等多因子檢測試劑盒(流式熒光發光法) | FLIA Kit for Antigen Detection. |
參考文獻
雜志 | 參考文獻 |
J Orthop Res | Interleukin‐6 and interleukin‐6 receptor expression, localization, and involvement in pain‐sensing neuron activation in a mouse intervertebral disc injury model [PubMed: 26010074] |
Gene.? | The IL6R gene polymorphisms are associated with sIL-6R, IgE and lung function in Chinese patients with asthma [Pubmed:26997259] |
Cytokine | Interleukin-6 participates in human pancreatic stellate cell activation and collagen I production via TGF-|?1/Smad pathway [33893003] |