中腦星形膠質細胞來源神經營養因子(MANF)活性蛋白
Active Mesencephalic Astrocyte Derived Neurotrophic Factor (MANF)
ARMET; ARP; Arginine Rich Mutated In Early Stage Tumors; Arginine-rich protein
- 編號APC300Hu01
- 物種Homo sapiens (Human，人) 相同的名稱，不同的物種。
- 緩沖液成份20mM Tris, 150mM NaCl緩沖液（pH8.0, 含有1mM EDTA, 1mM DTT, 0.01% SKL, 5% Trehalose和Proclin300）
- 性狀凍干粉
- 純度> 90%
- 等電點8.5
- 應用Cell?culture;?Activity?Assays.
- 下載 英文說明書 中文說明書
- 規格 10μg50μg 200μg 1mg 5mg
- 價格 ¥ 1771 ¥ 4428 ¥ 8856 ¥ 26568 ¥ 66420
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產品包裝(模擬)
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產品包裝(模擬)
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Figure. Gene Sequencing (Extract)
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Figure. SDS-PAGE
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Figure. Western Blot
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通過ISO 9001、ISO 13485質量體系認證
活性實驗
MANF (Mesencephalic astrocyte-derived neurotrophic factor) is a prosurvival protein that protects the cells when applied intracellularly in vitro or extracellularly in vivo. Recently, MANF has also been proven to inhibit cell proliferation and ER stress-induced cell death and also affects cell size and morphology under certain conditions. Thus the bioactivity of human recombinant MANF were detected using U87-MG. Briefly,?U87-MG cells were seeded into triplicate wells of 96-well plates at a density of 2,000 cells/well and allowed to attach overnight, then the medium was replaced with serum-free standard DMEM?prior to the addition of various concentrations of MANF. After incubated for 48h,?cells were observed by inverted microscope and were shown in Figure 1. It was obvious that MANF inhibited cell proliferation of U87-MG and affected cell size and morphology. (A)U7-MG cells cultured in DMEM, stimulated with 0.01ng/mL MANF for 48h;
(B)Unstimulated U87-MG cells cultured in DMEM for 48h.
Figure 1. Inhibition of U87-MG cell proliferation after stimulated with MANF.
用法
Reconstitute in 20mM Tris, 150mM NaCl (PH8.0) to a concentration of 0.1-1.0 mg/mL. Do not vortex.
儲存
避免反復凍融。2-8°C不超過一個月，-80°C不超過12個月。
穩定性
熱穩定性以損失率顯示。損失率是由加速降解試驗決定，具體方法如下：在37°C孵育48小時，沒有顯著的降解或者沉淀產生。保質期內，在適當的條件下存儲，損失率低于5%。
增值服務
相關產品
| 編號 | 適用物種：Homo sapiens (Human，人) | 應用(僅供研究使用，不用于臨床診斷！) |
| APC300Hu61 | 中腦星形膠質細胞來源神經營養因子(MANF)活性蛋白 | Cell?culture;?Activity?Assays. |
| EPC300Hu61 | 中腦星形膠質細胞來源神經營養因子(MANF)真核蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| APC300Hu01 | 中腦星形膠質細胞來源神經營養因子(MANF)活性蛋白 | Cell?culture;?Activity?Assays. |
| RPC300Hu01 | 中腦星形膠質細胞來源神經營養因子(MANF)重組蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| PAC300Hu01 | 中腦星形膠質細胞來源神經營養因子(MANF)多克隆抗體 | WB; IHC; ICC; IP. |
| MAC300Hu25 | 中腦星形膠質細胞來源神經營養因子(MANF)單克隆抗體 | WB |
| MAC300Hu21 | 中腦星形膠質細胞來源神經營養因子(MANF)單克隆抗體 | WB; IHC; ICC; IP. |
| MAC300Hu23 | 中腦星形膠質細胞來源神經營養因子(MANF)單克隆抗體 | WB; IHC; ICC; IP. |
| MAC300Hu24 | 中腦星形膠質細胞來源神經營養因子(MANF)單克隆抗體 | WB; IHC; ICC; IP. |
| MAC300Hu27 | 中腦星形膠質細胞來源神經營養因子(MANF)單克隆抗體 | WB; IHC; ICC; IP. |
| MAC300Hu22 | 中腦星形膠質細胞來源神經營養因子(MANF)單克隆抗體 | WB,IHC |
| MEC300Hu | 中腦星形膠質細胞來源神經營養因子(MANF)檢測試劑盒(酶聯免疫吸附試驗法,小樣本) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| SEC300Hu | 中腦星形膠質細胞來源神經營養因子(MANF)檢測試劑盒(酶聯免疫吸附試驗法) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| LMC300Hu | 中腦星形膠質細胞來源神經營養因子(MANF)等多因子檢測試劑盒(流式熒光發光法) | FLIA Kit for Antigen Detection. |
| KSC300Hu01 | 中腦星形膠質細胞來源神經營養因子(MANF)檢測試劑盒DIY材料(酶聯免疫吸附試驗法) | Main materials for "Do It(ELISA Kit) Yourself" |
參考文獻
| 雜志 | 參考文獻 |
| PLoS One. | Molecular Effects of Doxycycline Treatment on Pterygium as Revealed by Massive Transcriptome Sequencing [PubMed: PMC3378547] |
| Endocr J. | Circulating mesencephalic astrocyte-derived neurotrophic factor is increased in newly diagnosed prediabetic and diabetic patients, and is associated with insulin resistance. [pubmed:28216543] |
| Journal of Steroid Biochemistry and Molecular Biology | Depressive-like effect of prenatal exposure to DDT involves global DNA hypomethylation and impairment of GPER1/ESR1 protein levels but not ESR2 and AHR/ARNT signaling [pubmed:28263910] |
| Endocrine Journal | Circulating mesencephalic astrocyte-derived neurotrophic factor is increased in newly diagnosed prediabetic and diabetic patients, and is associated with insulin resistance [10.1507:endocrj.EJ16-0472] |
| Nature?Metabolism | MANF regulates metabolic and immune homeostasis in ageing and protects against liver damage [] |
| J Exp Med | Feeding-induced hepatokine, Manf, ameliorates diet-induced obesity by promoting adipose browning via p38 MAPK pathway [33856409] |
| J Inflamm Res | The Potential Protective Effect of Mesencephalic Astrocyte-Derived Neurotrophic Factor on Post-Operative Delirium via Inhibiting Inflammation and Microglia?… [34234505] |
