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視黃醇結合蛋白4(RBP4)活性蛋白

Active Retinol Binding Protein 4 (RBP4)

PRBP; RBP; Plasma retinol-binding protein; Retinol Binding Protein 4, Plasma

  • 視黃醇結合蛋白4(RBP4)活性蛋白 產品包裝(模擬)
  • 視黃醇結合蛋白4(RBP4)活性蛋白 產品包裝(模擬)
  • APA929Hu01.jpg Figure. SDS-PAGE
  • Certificate 通過ISO 9001、ISO 13485質量體系認證

活性實驗

Retinol-binding protein 4 (RBP4) is the specific carrier for retinol (also known as vitamin A), and is responsible for the conversion of unstable and insoluble retinol in aqueous solution into stable and soluble complex in plasma through their tight interaction. As a member of the lipocalin superfamily, RBP4 containing a β-barrel structure with a well-defined cavity is secreted from the liver, and in turn delivers retinol from the liver stores to the peripheral tissues. In plasma, the RBP4-retinol complex interacts with transthyretin (TTR), and this binding is crucial for preventing RBP4 excretion through the kidney glomeruli. RBP4 expressed from an ectopic source efficiently delivers retinol to the eyes, and its deficiency affects night vision largely. Recently, RBP4 as an adipokine, is found to be expressed in adipose tissue and correlated with obesity, insulin resistance (IR) and type 2 diabetes (T2DM). The activity of recombinant human RBP4 was measured by its ability to bind all-trans retinoic acid. The binding of retinoic acid results in the quenching of Trp fluorescence in RBP4. RBP4 was diluted to 50 ug/ml in 50 mM Tris, 10 mM CaCl2, 150 mM NaCl, pH 7.5 (assay buffer) and the retinoic acid was diluted to 100, 30, 10, 3 and 1 μM in 95% ethanol. Mixing 112.5 μL of 50 μg/mL rhRBP4 and 12.5 μL of retinoic acid serial dilutions in microtubes and a blank containing 112.5 μL of 50 μg/mL rhRBP4 and 12.5 μL of 95% ethanol, then incubate at room temperature for 30 minutes. Loading 100 ul of the reaction mixtures and blank and read at excitation and emission wavelengths of 280 nm and 340 nm (top read), respectively, in endpoint mode. The result was shown in figure 1, the 50% binding concentration (BC50) is >30 μM.

用法

Reconstitute in 10mM PBS (pH7.4) to a concentration of 0.1-1.0 mg/mL. Do not vortex.

儲存

避免反復凍融。2-8°C不超過一個月,-80°C不超過12個月。

穩定性

熱穩定性以損失率顯示。損失率是由加速降解試驗決定,具體方法如下:在37°C孵育48小時,沒有顯著的降解或者沉淀產生。保質期內,在適當的條件下存儲,損失率低于5%。

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編號 適用物種:Homo sapiens (Human,人) 應用(僅供研究使用,不用于臨床診斷!)
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參考文獻

雜志 參考文獻
Diabetes Technology & Therapeutics Proteomic Identification of Human Urinary Biomarkers in Diabetes Mellitus Type 2 [Liebert: 20100078]
Journal of Pharmaceutical and Biomedical Analysis Effect of high dose thiamine on the levels of urinary protein biomarkers in diabetes mellitus type 2 [PubMed: 21130593]
Evid Based Complement Alternat Med Quercetin Protects against Cadmium-Induced Renal Uric Acid Transport System Alteration and Lipid Metabolism Disorder in Rats [PubMed: PMC3368504]
Journal of Endocrinology Combined galanin with insulin improves insulin sensitivity of diabetic rat muscles [Pubmed:24501381]
J Endocrinol Central alarin ameliorated insulin resistance of adipocytes in type 2 diabetic rats [Pubmed:25240061]
Peptides Effect of endogenous galanin on glucose transporter 4 expression in cardiac muscle of type 2 diabetic rats [Pubmed:25445608]
Obesity Surgery Long-term Effect of Ileal Transposition on Adipokine Serum Level in Zucker (Orl)-Lepr fa Fatty Rats [Pubmed:25697126]
Clin Exp Nephrol Differentially expressed urinary biomarkers in children with idiopathic nephrotic syndrome [PubMed: 26351173]
Experimental and Therapeutic Medicine Expression of retinol binding protein 4 and nuclear factor-??B in diabetic rats with atherosclerosis and the intervention effect of pioglitazone [Pubmed:27446311]
Experimental and Therapeutic Medicine Expression?of?retinol?binding?protein?4?and?nuclear?factor-κB in?diabetic?rats?with?atherosclerosisand the?intervention?effect?of?pioglitazone. [pubmed:27446311]
Journal of Chromatography A Application of a new procedure for liquid chromatography/mass spectrometry profiling of plasma amino acid-related metabolites and untargeted shotgun proteomics to identify mechanisms and biomarkers of calcific aortic stenosis [S0021967317311858]
BMC Cancer Screening for immune-potentiating antigens from hepatocellular carcinoma patients after radiofrequency ablation by serum proteomic analysis [Pubmed:29386009]
Nutrition &?Diabetes Lack of pronounced changes in the expression of fatty acid handling proteins in adipose tissue and plasma of morbidly obese humans [Pubmed:29335416]
Molecular?Medicine?Reports RBP4 regulates trophoblastic cell proliferation and invasion via the PI3K/AKT signaling pathway [Pubmed:30015949]
Relationship between retinol and risk of diabetic retinopathy: a case-control study []
Scientific Reports Retinol binding protein 4 abundance in plasma and tissues is related to body fat deposition in cattle [Pubmed: 31147589]
Potassium supplementation blunts the effects of high salt intake on serum retinol‐binding protein 4 levels in healthy individuals []
Docosahexaenoic acid (DHA), a nutritional supplement, modulates steroid insensitivity in asthma []
Eur J Nutr The dietary inflammatory index is associated with anti-and pro-inflammatory adipokines in Brazilian schoolchildren [33575861]
Eur J Pediatr Pro-and anti-inflammatory adipokines are associated with cardiometabolic risk markers in Brazilian schoolchildren [33834274]
Sci Rep Ileal transposition helps to regulate plasma hepatokine levels in obese Zucker (Crl: ZUC (ORL)-Lepr fa) rats [33833309]
Int Arch Occup Environ Health Biomarkers of cadmium exposure and renal function in estuarine adult villagers [34773507]
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