促腎上腺皮質激素(ACTH)檢測試劑盒(化學發光免疫分析法)
CLIA Kit for Adrenocorticotropic Hormone (ACTH)
Corticotropin; Adrenocorticotropin
- 編號CCA836Hu
- 物種Homo sapiens (Human，人) 相同的名稱，不同的物種。
- 實驗方法競爭抑制
- 反應時長2h
- 檢測范圍3.9-1,000pg/mL
- 靈敏度最小可檢測劑量小于等于1.5pg/mL.
- 樣本類型serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
- 下載 英文說明書 中文說明書
- 規格 48T96T 96T*5 96T*10 96T*100
- 價格 ¥ 3528 ¥ 5040 ¥ 22680 ¥ 42840 ¥ 352800
- 欲了解實際交易價格和更多情況，請與當地經銷商聯系!
-
產品包裝(模擬)
-
產品包裝(模擬)
-
實驗結果圖
-
標準曲線圖
-
通過ISO 9001、ISO 13485質量體系認證
特異性
本試劑盒用于檢測促腎上腺皮質激素(ACTH)，經檢測與其它相似物質無明顯交叉反應。
由于受到技術及樣本來源的限制，不可能完成對所有相關或相似物質交叉反應檢測，因此本試劑盒有可能與未經檢測的其它物質有交叉反應。
回收率
分別于定值血清及血漿樣本中加入一定量的促腎上腺皮質激素(ACTH)（加標樣品），重復測定并計算其均值，回收率為測定值與理論值的比率。
| 樣本 | 回收率范圍(%) | 平均回收率(%) |
| serum(n=5) | 79-101 | 89 |
| EDTA plasma(n=5) | 79-99 | 85 |
| heparin plasma(n=5) | 87-98 | 91 |
精密度
精密度用樣品測定值的變異系數CV表示。CV(%) = SD/mean×100
批內差：取同批次試劑盒對低、中、高值定值樣本進行定量檢測,每份樣本連續測定20 次，分別計算不同濃度樣本的平均值及SD值。
批間差：選取3個不同批次的試劑盒分別對低、中、高值定值樣本進行定量測定，每個樣本使用同一試劑盒重復測定8次，分別計算不同濃度樣本的平均值及SD值。
批內差： CV<10%
批間差： CV<12%
線性
在定值血清及血漿樣本內加入適量的促腎上腺皮質激素(ACTH)，并倍比稀釋成1:2，1:4，1:8，1:16的待測樣本，線性范圍即為稀釋后樣本中促腎上腺皮質激素(ACTH)含量的測定值與理論值的比率。
| 樣本 | 1:2 | 1:4 | 1:8 | 1:16 |
| serum(n=5) | 99-105% | 97-104% | 93-101% | 81-90% |
| EDTA plasma(n=5) | 78-98% | 83-97% | 81-101% | 89-96% |
| heparin plasma(n=5) | 78-98% | 91-105% | 81-96% | 95-102% |
穩定性
經測定，試劑盒在有效期內按推薦溫度保存，其活性降低率小于5%。
為減小外部因素對試劑盒破壞前后檢測值的影響，實驗室的環境條件需盡量保持一致，尤其是實驗室內溫度、濕度及溫育條件。其次由同一實驗員來進行操作可減少人為誤差。
實驗流程
1. 實驗前標準品、試劑及樣本的準備；
2. 加樣（標準品及樣本）50µL，
加入50µL檢測液A(臨用前配制);
37°C溫育1小時。
3. 洗板3次；
4. 加檢測溶液B100µL，37°C孵育30分鐘；
5. 洗板5次；
6. 加底物100µL，37°C孵育10分鐘；
7. 讀數。
實驗原理
本試劑盒應用競爭抑制酶聯免疫分析法測定標本中待測物質水平。將促腎上腺皮質激素(ACTH)單克隆抗體包被微孔板，制成固相載體，往包被抗體的微孔中同時加入生物素標記的抗原和待測抗原（標準品或樣本），待測抗原與生物素標記抗原對特異性抗體進行競爭結合。溫育后經洗滌去掉未結合物，然后加入HRP標記的親和素，再次徹底洗滌后加入底物。加入底物后會產生輝光型光信號。發射光強度和樣品中的促腎上腺皮質激素(ACTH)呈負相關。用化學發光儀測定相對光單位（RLU），計算樣品濃度。
相關產品
| 編號 | 適用物種：Homo sapiens (Human，人) | 應用(僅供研究使用，不用于臨床診斷！) |
| CPA836Hu11 | 促腎上腺皮質激素(ACTH)牛血清白蛋白偶聯物 | Immunogen; SDS-PAGE; WB. |
| CPA836Hu21 | 促腎上腺皮質激素(ACTH)卵白蛋白偶聯物 | Immunogen; SDS-PAGE; WB. |
| PAA836Hu08 | 促腎上腺皮質激素(ACTH)多克隆抗體 | ELISA, CLIA. / IHC-Fr, ICC, IP (predicted). |
| PAA836Hu02 | 促腎上腺皮質激素(ACTH)多克隆抗體 | ELISA, CLIA. / IHC-Fr, ICC, IP (predicted). |
| LAA836Hu81 | 促腎上腺皮質激素(ACTH)多克隆抗體(異硫氰酸熒光素標記) | WB; IHC; ICC; IF. |
| MAA836Hu21 | 促腎上腺皮質激素(ACTH)單克隆抗體 | WB; IHC; ICC; IP. |
| MAA836Hu28 | 促腎上腺皮質激素(ACTH)單克隆抗體 | ELISA, CLIA. / IHC-Fr, ICC, IP (predicted). |
| CEA836Hu | 促腎上腺皮質激素(ACTH)檢測試劑盒(酶聯免疫吸附試驗法) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| CCA836Hu | 促腎上腺皮質激素(ACTH)檢測試劑盒(化學發光免疫分析法) | Chemiluminescent immunoassay for Antigen Detection. |
| LMA836Hu | 促腎上腺皮質激素(ACTH)等多因子檢測試劑盒(流式熒光發光法) | FLIA Kit for Antigen Detection. |
| KSA836Hu11 | 促腎上腺皮質激素(ACTH)檢測試劑盒DIY材料(酶聯免疫吸附試驗法) | Main materials for "Do It (ELISA Kit) Yourself". |
參考文獻
| 雜志 | 參考文獻 |
| Respir Res. | Intracerebroventricular injection of leukotriene B4 attenuates antigen-induced asthmatic response via BLT1 receptor stimulating HPA-axis in sensitized rats. [PubMed: 20403205] |
| Toxicology Letters | Regulation of corticosterone secretion is modified by PFOS exposure at different levels of the hypothalamic–pituitary–adrenal axis in adult male rats [Pubmed:24440345] |
| Mol Med Rep. | Ursolic acid suppresses growth and adrenocorticotrophic hormone secretion in AtT20 cells as a potential agent targeting adrenocorticotrophic hormone-producing pituitary adenoma. [Pubmed:24682498] |
| Peptides | Differential stress response in rats subjected to chronic mild stress is accompanied by changes in CRH-family gene expression at the pituitary level [Pubmed:25236411] |
| Journal of Animal and Feed Sciences | Interdependence of the peripheral metabolism of glucocorticoids and thyroid hormones under calorie deficit in rats at different ages [Researchgate:Source] |
| International Journal of Immunopathology & Pharmacology | Sea buckthorn (Hippophae rhamnoides L.) oil protects against chronic stress-induced inhibitory function of natural killer cells in rats [PubMed: 26684638] |
| Cell Tissue Res | CXCL10/CXCR3 signaling mediates inhibitory action by interferon-gamma on CRF-stimulated adrenocorticotropic hormone (ACTH) release [PubMed: 26572542] |
| Neuroscience Letters | Electroacupuncture reCavia (Guinea pig )late hypothalamic–pituitary–adrenal axis and enhance hippocampal serotonin system in a rat model of depression [Pubmed:26773866] |
| Neural Regeneration Research | Moxibustion?upreCavia (Guinea pig )lates?hippocampal?progranulin?expression. [Pubmed:27212922] |
| Environmental Science and Pollution Research | Lycopene ameliorates atrazine-induced oxidative damage in adrenal cortex of male rats by activation of the Nrf2/HO-1 pathway [Pubmed:27102619] |
| Sci Rep. | Hedgehog signaling activation induces stem cell proliferation and hormone release in the adult pituitary gland [Pubmed:27109116] |
| Sci Rep. | High activity of the stress promoter contributes to susceptibility to stress in the tree shrew [Pubmed:27125313] |
| brain research bulletin | Activation of ATP-sensitive potassium channel by iptakalim normalizes stress-induced HPA axis disorder and depressive behaviour by alleviating inflammation and oxidative stress in mouse hypothalamus. [pubmed:28161195] |
| 中國臨床藥理學與治療學 | 肉蔻五味丸抗抑郁作用及機制研究 [9787] |
| Neuroscience | Heat stress-induced neuroinflammation and aberration in monoamine levels in hypothalamus are associated with temperature dysregulation [pubmed:28663093] |
| Toxicology | Mixture effects of azole fungicides on the adrenal gland in a broad dose range [pubmed:28454766] |
| Biomedicine & Pharmacotherapy | Triptolide suppresses growth and hormone secretion in murine pituitary corticotroph tumor cells via NF-kappaB signaling pathway [pubmed:28892788] |
| Hormones and?Behavior | Acth-induced model of depression resistant to tricyclic antidepressants: Neuroendocrine and behavioral changes and influence of long-term magnesium?… [Pubmed:30025718] |
| Frontiers in Pharmacology | Vaccinium bracteatum Leaf Extract Reverses Chronic Restraint Stress-Induced Depression-Like Behavior in Mice: Regulation of Hypothalamic-Pituitary?… [Pubmed:30038568] |
| Molecular and Cellular Endocrinology | Metformin suppresses growth and adrenocorticotrophic hormone secretion in mouse pituitary corticotroph tumor AtT20 cells [10.1016:j.mce.2018.07.007] |
| Frontiers in?Behavioral?Neuroscience | Chronic hippocampal abnormalities and blunted HPA axis in an animal model of repeated unpredictable stress [Pubmed:30079015] |
| Placenta | Human placentophagy: Effects of dehydration and steaming on hormones, metals and bacteria in placental tissue [Pubmed:29941176] |
| BMC?Veterinary?Research | Hypothalamus-pituitary-adrenal axis involves in anti-viral ability through regulation of immune response in piglets infected by highly pathogenic porcine?… [Pubmed:29540178] |
| European Journal of Applied Physiology | Comparison between men and women of volume regulating hormones and aquaporin-2 excretion following graded central hypovolemia [Pubmed: 30564880] |
| Cyber Leninka | ТЭС-ТЕРАПИЯ КАК МЕТОД ПРЕДУПРЕЖДЕНИЯ ДЕЗАДАПТАЦИИ У САМЦОВ КРЫС С ВЫСОКОЙ СТРЕССОУСТОЙЧИВОСТЬЮ [] |
| Serbian Journal of Experimental and Clinical Research | Assessment of the TDCS Influence on Stress-Induced Disorders in Rats with Low Stress Sustainability and Endurance [] |
| Scientific Reports | Mice lacking Casp1, Ifngr and Nos2 genes exhibit altered depressive-and anxiety-like behaviour, and gut microbiome composition [Pubmed: 31015500] |
| alcoholism-clinical and experimental research | Persistent changes in stress‐regulatory genes in pregnant woman or a child with prenatal alcohol exposure [Pubmed: 31329297] |
| Hormones and Behavior | Hypothalamus-pituitary-adrenal axis imbalance and inflammation contribute to sex differences in separation-and restraint-induced depression [Pubmed: 32165183] |
| Prog Neuropsychopharmacol Biol Psychiatry | Progress in Neuropsychopharmacology & Biological Psychiatry [Pubmed: 17134810] |
| Evid Based Complement Alternat Med | So-Ochim-Tang-Gamibang, a Traditional Herbal Formula, Ameliorates Depression by Regulating Hyperactive Glucocorticoid Signaling In Vitro and In Vivo [Pubmed: 33224257] |
| PROGRESS IN NEURO-PSYCHOPHARMACOLOGY & BIOLOGICAL PSYCHIATRY | Repeated unpredictable stress and social isolation induce chronic HPA axis dysfunction and persistent abnormal fear memory [Pubmed: 32682873] |
| Aging Cell | Altered glucocorticoid metabolism represents a feature of macroph‐aging [Pubmed: 32463582] |
| neurochemical research | Anxiolytic and Anti?depressive Like Effects of Translocator Protein (18 kDa) Ligand YL?IPA08 in a Rat Model of Postpartum Depression [Pubmed: 32383026] |
| Behavioral Ecology and Sociobiology? | Exposure to eagle owl feces induces anti-predator behavior, physiology, and hypothalamic gene responses in a subterranean rodent, the plateau zokor (Eospalax?… [] |
| EUROPEAN CHILD & ADOLESCENT PSYCHIATRY | Increased serum brain-derived neurotrophic factor and adrenocorticotropic hormone levels are associated with obsessive compulsive disorder in medication?free??- [33389158] |
| International Journal of?Nanomedicine | Water-Soluble Carbon Dots in Cigarette Mainstream Smoke: Their Properties and the Behavioural, Neuroendocrinological, and Neurotransmitter Changes They ?- [33762821] |
| J Am Heart Assoc | Post?\Cardiac Arrest Hydrocortisone Use Ameliorates Cardiac Mitochondrial Injury in a Male Rat Model of Ventricular Fibrillation Cardiac Arrest [33960200] |
| Effects of Propofol versus Sevoflurane on Postoperative Pain and Neuroendocrine Stress Response in Oocyte Pickup Patients [33936350] |
留言咨詢
