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補體成分3(C3)檢測試劑盒(化學發光免疫分析法)

CLIA Kit for Complement Component 3 (C3)

ASP; CPAMD1; Complement Protein C3; C3 and PZP-like alpha-2-macroglobulin domain-containing protein 1; C3a anaphylatoxin; Acylation stimulating protein

  • 補體成分3(C3)檢測試劑盒(化學發光免疫分析法) 產品包裝(模擬)
  • 補體成分3(C3)檢測試劑盒(化學發光免疫分析法) 產品包裝(模擬)
  • 補體成分3(C3)檢測試劑盒(化學發光免疫分析法) 實驗結果圖
  • SCA861Ra.jpg 標準曲線圖
  • Certificate 通過ISO 9001、ISO 13485質量體系認證

特異性

本試劑盒用于檢測補體成分3(C3),經檢測與其它相似物質無明顯交叉反應。
由于受到技術及樣本來源的限制,不可能完成對所有相關或相似物質交叉反應檢測,因此本試劑盒有可能與未經檢測的其它物質有交叉反應。

回收率

分別于定值血清及血漿樣本中加入一定量的補體成分3(C3)(加標樣品),重復測定并計算其均值,回收率為測定值與理論值的比率。

樣本 回收率范圍(%) 平均回收率(%)
serum(n=5) 86-93 89
EDTA plasma(n=5) 78-101 83
heparin plasma(n=5) 87-102 91

精密度

精密度用樣品測定值的變異系數CV表示。CV(%) = SD/mean×100
批內差:取同批次試劑盒對低、中、高值定值樣本進行定量檢測,每份樣本連續測定20 次,分別計算不同濃度樣本的平均值及SD值。
批間差:選取3個不同批次的試劑盒分別對低、中、高值定值樣本進行定量測定,每個樣本使用同一試劑盒重復測定8次,分別計算不同濃度樣本的平均值及SD值。
批內差: CV<10%
批間差: CV<12%

線性

在定值血清及血漿樣本內加入適量的補體成分3(C3),并倍比稀釋成1:2,1:4,1:8,1:16的待測樣本,線性范圍即為稀釋后樣本中補體成分3(C3)含量的測定值與理論值的比率。

樣本 1:2 1:4 1:8 1:16
serum(n=5) 98-105% 97-104% 78-104% 81-94%
EDTA plasma(n=5) 95-102% 87-99% 92-99% 90-98%
heparin plasma(n=5) 86-102% 98-105% 92-101% 78-98%

穩定性

經測定,試劑盒在有效期內按推薦溫度保存,其活性降低率小于5%。
為減小外部因素對試劑盒破壞前后檢測值的影響,實驗室的環境條件需盡量保持一致,尤其是實驗室內溫度、濕度及溫育條件。其次由同一實驗員來進行操作可減少人為誤差。

實驗流程

1. 實驗前標準品、試劑及樣本的準備;
2. 加樣(標準品及樣本)100µL,37°C孵育1小時;
3. 吸棄,加檢測溶液A100μL,37°C孵育1小時;
4. 洗板3次;
5. 加檢測溶液B100µL,37°C孵育30分鐘;
6. 洗板5次;
7. 加底物100µL,37°C孵育10分鐘;
8. 讀數。

實驗原理

將補體成分3(C3)抗體包被于96孔微孔板中,制成固相載體,向微孔中分別加入標準品或標本,其中的補體成分3(C3)與連接于固相載體上的抗體結合,然后加入生物素化的補體成分3(C3)抗體,將未結合的生物素化抗體洗凈后,加入HRP標記的親和素,再次徹底洗滌后加入底物。加入底物后會產生輝光型光信號。發射光強度和樣品中的補體成分3(C3)呈正相關。用化學發光儀測定相對光單位(RLU),計算樣品濃度。

相關產品

編號 適用物種:Rattus norvegicus (Rat,大鼠) 應用(僅供研究使用,不用于臨床診斷!)
RPA861Ra01 補體成分3(C3)重組蛋白 Positive Control; Immunogen; SDS-PAGE; WB.
PAA861Ra01 補體成分3(C3)多克隆抗體 WB; IHC; ICC; IP.
LAA861Ra71 補體成分3(C3)多克隆抗體(生物素標記) WB; IHC; ICC.
LAA861Ra81 補體成分3(C3)多克隆抗體(異硫氰酸熒光素標記) WB; IHC; ICC; IF.
MAA861Ra21 補體成分3(C3)單克隆抗體 WB; IHC; ICC; IP.
SEA861Ra 補體成分3(C3)檢測試劑盒(酶聯免疫吸附試驗法) Enzyme-linked immunosorbent assay for Antigen Detection.
SCA861Ra 補體成分3(C3)檢測試劑盒(化學發光免疫分析法) Chemiluminescent immunoassay for Antigen Detection.
LMA861Ra 補體成分3(C3)等多因子檢測試劑盒(流式熒光發光法) FLIA Kit for Antigen Detection.

參考文獻

雜志 參考文獻
Artificial Organs Experimental Research With Synthetic Copolymer-Coated Cardiopulmonary Bypass Circuits: Inflammatory and Thrombogenicity Analysis [Wiley: source]
Journal of Clinical Immunology Complement activation contributes to the injury and outcome of kidney in human anti-glomerular basement membrane disease. [PubMed: 22941511]
Lipids in Health and Disease Beneficial effects of omega-3 fatty acids in the proteome of high-density lipoprotein proteome [PubMed: PMC3480889]
Am J Reprod Immunol Human IgG Antinuclear Antibodies Induce Pregnancy Loss in Mice by Increasing Immune Complex Deposition in Placental Tissue: In Vivo Study [PubMed: 26388133]
Veterinary Immunology and Immunopathology Oral administration of heat-inactivated Mycobacterium bovis reduces the response of farmed red deer to avian and bovine tuberculin [Pubmed:27032499]
International Journal of Molecular Sciences Identification of Serum Peptidome Signatures of Non-Small Cell Lung Cancer [Pubmed:27043541]
Food Chem. Comparative proteomics of milk fat globule membrane in goat colostrum and mature milk [Pubmed:27173528]
Scientific Reports Generation of complement protein C3 deficient pigs by CRISPR/Cas9-mediated gene targeting. [pubmed:28694465]
Veterinary Microbiology The response of red deer to oral administration of heat-inactivated Mycobacterium bovis and challenge with a field strain. [pubmed:28888638]
Proteomics?Clinical?Applications iTRAQ‐Based Proteomic Analysis Reveals Protein Profile in Plasma from Children with Autism [Pubmed:29274201]
Nanomaterials An OMV-Based Nanovaccine Confers Safety and Protection against Pathogenic Escherichia coli via Both Humoral and Predominantly Th1 Immune Responses in?… [Pubmed: 33233490]
Diagnostics Systemic Alterations of Immune Response-Related Proteins during Glaucoma Development in the Murine Model DBA/2J [Pubmed: 32585848]
Scientific Reports The bovine cumulus proteome is influenced by maturation condition and maturational competence of the oocyte [Pubmed: 32555221]
Int Immunopharmacol Complement component 3 prevents imiquimod-induced psoriatic skin inflammation by inhibiting apoptosis in mice [Pubmed: 32535539]
Italian Journal of Animal Science Effects of chitooligosaccharide supplementation on laying performance, egg quality, blood biochemistry, antioxidant capacity and immunity of laying hens during the?… []
Dev Comp Immunol A deep-sea pathogenic Bacillus subtilis isolate employs different strategies to escape the killing of teleost and murine complements [33545212]
Animal Effect of alfalfa hay and starter feed supplementation on caecal microbiota and fermentation, growth, and health of yak calves [33610512]
British Journal of Pharmacology The suppression of Brd4 inhibits peripheral plasma cell differentiation and exhibits therapeutic potential for systemic lupus erythematosus []
Scientific Reports Maternal Serum Proteomic Profiles of Pregnant Women With Type 1 Diabetes [34940989]
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