半胱氨酸蛋白酶抑制劑B(CSTB)重組蛋白
Recombinant Cystatin B (CSTB)
Cys-B; CST6; EPM1; PME; STFB; CPI-B; Stefin B; Liver thiol proteinase inhibitor
- 編號RPJ322Bo01
- 物種Bos taurus; Bovine (Cattle,牛) 相同的名稱,不同的物種。
- 來源原核表達
- 宿主E.coli
- 內毒素水平<1.0EU/µg(LAL法測定)
- 亞細胞定位細胞質
- 預測分子量12.4kDa
- 實際分子量15kDa(差異分析請參閱說明書)
- 片段與標簽Met1~Phe98 with N-terminal His Tag
- 緩沖液成份20mM Tris, 150mM NaCl緩沖液(pH8.0, 含有1mM EDTA, 1mM DTT, 0.01% SKL, 5% Trehalose和Proclin300)
- 性狀凍干粉
- 純度> 97%
- 等電點6.8
-
應用
Positive Control; Immunogen; SDS-PAGE; WB.
如果需要有生物活性的蛋白,請參見活性蛋白。 - 下載 英文說明書 中文說明書
- 規格 10μg50μg 200μg 1mg 5mg
- 價格 ¥ 912 ¥ 2280 ¥ 4560 ¥ 13680 ¥ 34200
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序列
用法
Reconstitute in 20mM Tris, 150mM NaCl (pH8.0) to a concentration of 0.1-1.0 mg/mL. Do not vortex.
儲存
避免反復凍融。2-8°C不超過一個月,-80°C不超過12個月。
穩定性
熱穩定性以損失率顯示。損失率是由加速降解試驗決定,具體方法如下:在37°C孵育48小時,沒有顯著的降解或者沉淀產生。保質期內,在適當的條件下存儲,損失率低于5%。
增值服務
相關產品
編號 | 適用物種:Bos taurus; Bovine (Cattle,牛) | 應用(僅供研究使用,不用于臨床診斷!) |
RPJ322Bo01 | 半胱氨酸蛋白酶抑制劑B(CSTB)重組蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
PAJ322Bo01 | 半胱氨酸蛋白酶抑制劑B(CSTB)多克隆抗體 | WB; IHC; ICC; IP. |
LAJ322Bo81 | 半胱氨酸蛋白酶抑制劑B(CSTB)多克隆抗體(異硫氰酸熒光素標記) | WB; IHC; ICC; IF. |
LAJ322Bo71 | 半胱氨酸蛋白酶抑制劑B(CSTB)多克隆抗體(生物素標記) | WB; IHC; ICC. |
參考文獻
雜志 | 參考文獻 |
AIDS. | Cathepsin B and cystatin B in HIV-seropositive women are associated with infection and HIV-1-associated neurocognitive disorders [PubMed: 23291538] |
International Journal of Circumpolar Health | Evaluation of serum procathepsin B, cystatin B and cystatin C as possible biomarkers of ovarian cancer [PubMed: PMC3754495] |
PLoS One. | Dysregulation of Macrophage-Secreted Cathepsin B Contributes to HIV-1-Linked Neuronal Apoptosis [PubMed: PMC3365072] |
Journal of Neuroimmune Pharmacology | Cocaine potentiates cathepsin B secretion and neuronal apoptosis from HIV-infected macrophages [Pubmed:25209871] |
European Journal of Cancer Supplements | A91: Endogenous inhibitors of cysteine proteases and preform of cathepsin B in cancer of reproductive system [Science: Article] |
Annals of Clinical Biochemistry | Diagnostic specificity and sensitivity of PIVKAII, GP3, CSTB, SCCA1 and HGF for the diagnosis of hepatocellular carcinoma in patients with alcoholic liver cirrhosis. [pubmed:28766361] |
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