細胞色素P450家族成員1A2(CYP1A2)重組蛋白
Recombinant Cytochrome P450 1A2 (CYP1A2)
CP12; P3-450; P450(PA); Cytochrome P450,Family 1,Subfamily A,Polypeptide 2
- 編號RPD294Ra01
- 物種Rattus norvegicus (Rat,大鼠) 相同的名稱,不同的物種。
- 來源原核表達
- 宿主E.coli
- 內毒素水平<1.0EU/µg(LAL法測定)
- 亞細胞定位內質網腔
- 預測分子量33.0KDa
- 實際分子量33kDa(差異分析請參閱說明書)
- 片段與標簽Val192~Glu444 with N-terminal His Tag
- 緩沖液成份20mM Tris, 150mM NaCl緩沖液(pH8.0, 含有1mM EDTA, 1mM DTT, 0.01% SKL, 5% Trehalose和Proclin300)
- 性狀凍干粉
- 純度> 97%
- 等電點6.1
-
應用
Positive Control; Immunogen; SDS-PAGE; WB.
如果需要有生物活性的蛋白,請參見活性蛋白。 - 下載 英文說明書 中文說明書
- 規格 10μg50μg 200μg 1mg 5mg
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序列
用法
Reconstitute in 20mM Tris, 150mM NaCl (pH8.0) to a concentration of 0.1-1.0 mg/mL. Do not vortex.
儲存
避免反復凍融。2-8°C不超過一個月,-80°C不超過12個月。
穩定性
熱穩定性以損失率顯示。損失率是由加速降解試驗決定,具體方法如下:在37°C孵育48小時,沒有顯著的降解或者沉淀產生。保質期內,在適當的條件下存儲,損失率低于5%。
增值服務
相關產品
編號 | 適用物種:Rattus norvegicus (Rat,大鼠) | 應用(僅供研究使用,不用于臨床診斷!) |
RPD294Ra01 | 細胞色素P450家族成員1A2(CYP1A2)重組蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
PAD294Ra01 | 細胞色素P450家族成員1A2(CYP1A2)多克隆抗體 | WB; IHC; ICC; IP. |
MAD294Ra21 | 細胞色素P450家族成員1A2(CYP1A2)單克隆抗體 | WB; IHC; ICC; IP. |
SED294Ra | 細胞色素P450家族成員1A2(CYP1A2)檢測試劑盒(酶聯免疫吸附試驗法) | Enzyme-linked immunosorbent assay for Antigen Detection. |
LMD294Ra | 細胞色素P450家族成員1A2(CYP1A2)等多因子檢測試劑盒(流式熒光發光法) | FLIA Kit for Antigen Detection. |
參考文獻
雜志 | 參考文獻 |
Environmental Monitoring and Assessment | Differential expression of HO-1 and CYP1A2 during up-regulation of ERK in stressed fish hepatocytes [Pubmed:25471622] |
The Journal of Nutrition | Apiaceous Vegetable Consumption Decreases PhIP-Induced DNA Adducts and Increases Methylated PhIP Metabolites in the Urine Metabolome in Rats1,2,4 [PubMed: 25733458] |
Molecular Nutrition Food Research | Phenethyl isothiocyanate and indole-3-carbinol from cruciferous vegetables, but not furanocoumarins from apiaceous vegetables, reduced PhIP-induced DNA adducts in Wistar rats [Pubmed:27133590] |
Molecular Nutrition &?Food?Research | Phenethyl isothiocyanate?and?indole-3-carbinol?from?cruciferous?vegetables, but not furanocoumarins from apiaceous?vegetables, reduced PhIP-induced DNA adducts in Wistar rats. [pubmed:27133590] |
?Journal of FisheriesSciences. com | Upregulation of HSP70 Extends Cytoprotection to Fish Brain under Xenobiotic Stress [b0407ebde042c4f61b728bc11a586e6c] |
Toxicology | Liver lobe and strain differences in the activity of murine cytochrome p450 enzymes [Pubmed:29879457] |
Stammesspezifische Unterschiede in der analgetischen Wirkung von Buprenorphin bei der Maus [] |
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