β-內啡肽(bEP)檢測試劑盒DIY材料(酶聯免疫吸附試驗法)
ELISA Kit DIY Materials for Beta-Endorphin (bEP)
b-EP
- 編號KSA806Ra11
- 物種Rattus norvegicus (Rat，大鼠) 相同的名稱，不同的物種。
- 試劑內容 包被抗體、生物素標記的競爭物、標準品、HRP標記的鏈霉親和素、TMB底物、96孔板
- 可檢測樣本Serum, plasma, tissue homogenates, cell lysates, cerebrospinal fluid, cell culture supernates and other biological fluids
- 可適用原理競爭抑制酶聯免疫吸附試驗法測抗原
- 可檢測范圍12.35-1000pg/mL
- 可適靈敏度4.62pg/mL
- 應用Main materials for "Do It (ELISA Kit) Yourself".
- 下載 英文說明書 中文說明書
- 規格 96T*596T*10 96T*20 96T*50 96T*100
- 價格 ¥ 7387 ¥ 12312 ¥ 22162 ¥ 43092 ¥ 73872
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產品包裝(模擬)
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產品包裝(模擬)
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通過ISO 9001、ISO 13485質量體系認證
特異性
試劑盒中的抗體對β-內啡肽(bEP)具有高靈敏度和高特異性，經檢測與其它相似物質無明顯交叉反應。
用法
1.用100μL每孔的工作濃度的包被抗體包被96孔板，4度孵育過夜或者37度孵育2小時；
2.洗板1次；
3.用200μL每孔的工作濃度的封閉液,37度孵育1.5小時；
4.洗板1次。酶標板即可用于ELISA實驗，后續使用方法與ELISA同。
儲存
抗體，標準品和HRP標記的鏈霉親和素在-20度保存，TMB需在4度保存，96孔板在室溫保存。所有組分保質期為1年，試劑一旦開封，在4度保存時間為1個月。
配套試劑
增值服務
相關產品
| 編號 | 適用物種：Rattus norvegicus (Rat，大鼠) | 應用(僅供研究使用，不用于臨床診斷！) |
| CPA806Ra21 | β-內啡肽(bEP)卵白蛋白偶聯物 | Immunogen; SDS-PAGE; WB. |
| PAA806Ra01 | β-內啡肽(bEP)多克隆抗體 | IHC; ICC; IP. |
| CEA806Ra | β-內啡肽(bEP)檢測試劑盒(酶聯免疫吸附試驗法) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| CCA806Ra | β-內啡肽(bEP)檢測試劑盒(化學發光免疫分析法) | Chemiluminescent immunoassay for Antigen Detection. |
| LMA806Ra | β-內啡肽(bEP)等多因子檢測試劑盒(流式熒光發光法) | FLIA Kit for Antigen Detection. |
| KSA806Ra11 | β-內啡肽(bEP)檢測試劑盒DIY材料(酶聯免疫吸附試驗法) | Main materials for "Do It (ELISA Kit) Yourself". |
參考文獻
| 雜志 | 參考文獻 |
| Experimental Dermatology | Endogenous μ-opioid peptides modulate immune response towards malignant melanoma [Publish: RD10279] |
| European Journal of Physiology | Physiology and cell biology of acupuncture observed in calcium signaling activated by acoustic shear wave [SpringerLink: tv015l3126833315] |
| International Journal of Pharmaceutical Science Invention ISSN(Online) | Effect of Ramadan fasting on endorphin and endocannabinoid level in serum, PBMC and macrophage [Ijpsi: Source] |
| Neurological Sciences | Evaluation of immune parameters in chronic migraine with medication overuse [Pubmed:24867859] |
| Brain Behav. | Cerebrospinal fluid and plasma b-endorphin levels in children with cerebral malaria. [pubmed:28413714] |
| Journal of Cosmet Dermatol. | Ultraviolet A photosensitivity profile of dexchlorpheniramine maleate and promethazine-based creams: Anti-inflammatory,antihistaminic, and skin barrier protection properties [DOI: 10.1111/jocd.12349] |
| International Journal of Nursing?Practice | Non‐pharmacological interventions during childbirth for pain relief, anxiety, and neuroendocrine stress parameters: A randomized controlled trial [Pubmed:29512230] |
| КОРРЕКЦИЯ ЦИТОКИНОВОГО И ГОРМОНАЛЬНОГО ДИСБАЛАНСА ПРИ ЛЕЧЕНИИ СТАБИЛЬНОЙ СТЕНОКАРДИИ НАПРЯЖЕНИЯ [10.25207:1608-6228-2018-25-3-51-55] | |
| Journal of Pediatric Neurosciences | Plasma and cerebrospinal fluid beta-endorphin levels show a strong association in children with cerebral malaria [] |
| Chinese Journal of Physiology | Blood dopamine level enhanced by caffeine in men after treadmill running [Pubmed: 31793465] |
| Free Radical Biology and Medicine | Blue light (λ= 453 nm) nitric oxide dependently induces β-endorphin production of human skin keratinocytes in-vitro and increases systemic β-endorphin levels in?… [Pubmed: 31553937] |
| ?Journal of Sexual Medicine | Between pleasure and pain: a pilot study on the biological mechanisms associated with BDSM interactions in dominants and submissives [Pubmed: 32044259] |
| JOURNAL OF AFFECTIVE DISORDERS | Pain Sensitivity and Plasma Beta-Endorphin in Adolescent Non-Suicidal Self-Injury [Pubmed: 32961416] |
| EUROPEAN NEUROPSYCHOPHARMACOLOGY | Serum β-endorphin levels are associated with addiction to suicidal behavior: a pilot study [Pubmed: 32855024] |
| J Burn Care Res | Efficacy of Acupuncture on Pain Mechanisms, Inflammatory Responses, and Wound Healing in the Acute Phase of Major Burns: An Experimental Study on Rats [34309681] |
| European Journal of Integrative Medicine | Effects of transcutaneous electrical nerve stimulation applied at different frequencies during labor on hormone levels, labor pain perception, and anxiety: a randomized?… [] |
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