降鈣素基因相關肽(CGRP)檢測試劑盒DIY材料(酶聯免疫吸附試驗法)
ELISA Kit DIY Materials for Calcitonin Gene Related Peptide (CGRP)
CALCB; CGRP2; CALC2; CGRP-II; Calcitonin Gene Related Peptide 2; Calcitonin 2; Calcitonin Related Polypeptide Beta
- 編號KSA876Hu11
- 物種Homo sapiens (Human，人) 相同的名稱，不同的物種。
- 試劑內容 包被抗體、生物素標記的競爭物、標準品、HRP標記的鏈霉親和素、TMB底物、96孔板
- 可檢測樣本serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
- 可適用原理競爭抑制酶聯免疫吸附試驗法測抗原
- 可檢測范圍12.35-1000pg/mL
- 可適靈敏度5.25pg/mL
- 應用Main materials for "Do It (ELISA Kit) Yourself".
- 下載 英文說明書 中文說明書
- 規格 96T*596T*10 96T*20 96T*50 96T*100
- 價格 ¥ 6048 ¥ 10080 ¥ 18144 ¥ 35280 ¥ 60480
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產品包裝(模擬)
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產品包裝(模擬)
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通過ISO 9001、ISO 13485質量體系認證
特異性
試劑盒中的抗體對降鈣素基因相關肽(CGRP)具有高靈敏度和高特異性，經檢測與其它相似物質無明顯交叉反應。
用法
1.用100μL每孔的工作濃度的包被抗體包被96孔板，4度孵育過夜或者37度孵育2小時；
2.洗板1次；
3.用200μL每孔的工作濃度的封閉液,37度孵育1.5小時；
4.洗板1次。酶標板即可用于ELISA實驗，后續使用方法與ELISA同。
儲存
抗體，標準品和HRP標記的鏈霉親和素在-20度保存，TMB需在4度保存，96孔板在室溫保存。所有組分保質期為1年，試劑一旦開封，在4度保存時間為1個月。
配套試劑
增值服務
相關產品
| 編號 | 適用物種：Homo sapiens (Human，人) | 應用(僅供研究使用，不用于臨床診斷！) |
| RPA876Hu01 | 降鈣素基因相關肽(CGRP)重組蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| CPA876Hu21 | 降鈣素基因相關肽(CGRP)卵白蛋白偶聯物 | Immunogen; SDS-PAGE; WB. |
| PAA876Hu01 | 降鈣素基因相關肽(CGRP)多克隆抗體 | WB; IHC; ICC; IP. |
| LAA876Hu71 | 降鈣素基因相關肽(CGRP)多克隆抗體(生物素標記) | WB; IHC; ICC. |
| MAA876Hu22 | 降鈣素基因相關肽(CGRP)單克隆抗體 | WB; IHC; ICC; IP. |
| CEA876Hu | 降鈣素基因相關肽(CGRP)檢測試劑盒(酶聯免疫吸附試驗法) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| MEA876Hu | 降鈣素基因相關肽(CGRP)檢測試劑盒(酶聯免疫吸附試驗法,小樣本) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| CCA876Hu | 降鈣素基因相關肽(CGRP)檢測試劑盒(化學發光免疫分析法) | Chemiluminescent immunoassay for Antigen Detection. |
| LMA876Hu | 降鈣素基因相關肽(CGRP)等多因子檢測試劑盒(流式熒光發光法) | FLIA Kit for Antigen Detection. |
| KSA876Hu11 | 降鈣素基因相關肽(CGRP)檢測試劑盒DIY材料(酶聯免疫吸附試驗法) | Main materials for "Do It (ELISA Kit) Yourself". |
參考文獻
| 雜志 | 參考文獻 |
| Evidence-Based Complementary and Alternative Medicine | Alstonia scholaris R. Br. Significantly Inhibits Retinoid-Induced Skin Irritation In Vitro and In Vivo. [Hindawi: 190370] |
| The Journal of Headache and Pain | Increased levels of CGRP in peripheral blood in women with chronic migraine: A reliable biological marker [PubMed: PMC3620076] |
| Headache: The Journal of Head and Face Pain | CGRP and VIP Levels as Predictors of Efficacy of Onabotulinumtoxin Type A in Chronic Migraine [Onlinelibrary: head.12372] |
| PLoS One. | Ultraviolet B Irradiation Reduces the Expression of Adiponectin in Ovarial Adipose Tissues through Endocrine Actions of Calcitonin Gene-Related Peptide-Induced Serum Amyloid A [Pubmed:Pmc4028234] |
| Int J Vasc Med. | Prognostic significance of circulating and endothelial progenitor cell markers in type 2 diabetic foot. [Pubmed:24624298] |
| Arch Gynecol Obstet. | Calcitonin gene-and parathyroid hormone-related peptides in normotensive and preeclamptic pregnancies: a nested case–control study [Pubmed:24943060] |
| Pain | OnabotulinumtoxinA decreases interictal CGRP plasma levels in patients with chronic migraine [PubMed: 25735000] |
| Molecular Medicine Reports | Exogenous α-calcitonin gene-related peptide attenuates lipopolysaccharide-induced acute lung injury in rats [PubMed: 25892548] |
| Annals of Allergy, Asthma and Immunology | Effect of acupuncture on house dust mite specific IgE, substance P, and symptoms in persistent allergic rhinitis [Pubmed:27156748] |
| Cephalalgia. | Relationship between serum levels of VIP, but not of CGRP, and cranial autonomic parasympathetic symptoms: A study in chronic migraine patients [Pubmed:27250233] |
| Cardiology.? | Transient Receptor Potential Vanilloid Subtype 1 Inhibits Inflammation and Apoptosis via the Release of Calcitonin Gene-Related Peptide in the Heart after Myocardial Infarction. [Pubmed:27144592] |
| J Headache Pain | Valproate ameliorates nitroglycerin-induced migraine in trigeminal nucleus caudalis in rats through inhibition of NF-кB [Pubmed:27150105] |
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| Naunyn Schmiedebergs Arch Pharmacol. | Opioid receptor agonists may favorably affect bone mechanical properties in rats with estrogen deficiency-induced osteoporosis. [pubmed:27896372] |
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| 9 | Probiotic Bifidobacterium longum NCC3001 Reduces Depression Scores and Alters Brain Activity: A Pilot Study in Patients With Irritable Bowel Syndrome [pubmed:28483500] |
| Journal of Clinical Investigation | CNS myeloid cells critically regulate heat hyperalgesia [Pubmed:29634489] |
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| BioRxiv?-?the?preprint?server?for?biology | RAMP1 in Kupffer cells is a critical regulator in immune-mediated hepatitis [10.1101:357582] |
| International?Journal?of?Pharmaceutical?Sciences?and?Research | MOTION SICKNESS INDUCES PHYSIOLOGICAL AND NEURONAL ALTERATIONS IN MOUSE MODEL [] |
| JOURNAL OF PERIODONTOLOGY | Periodontal inflammation is related to increased serum calcitonin gene‐related peptide (CGRP) levels in patients with chronic migraine [Pubmed: 31070784] |
| Molecular and Cellular Biochemistry | Rhynchophylline attenuates migraine in trigeminal nucleus caudalis in nitroglycerin-induced rat model by inhibiting MAPK/NF-кB signaling [Pubmed: 31420791] |
| Journal of Headache and Pain | Electrical stimulation of the superior sagittal sinus suppresses A-type K+ currents and increases P/Q-and T-type Ca 2+ currents in rat trigeminal ganglion?… [] |
| Respiratory Physiology & Neurobiology | Effects of nanoparticles on Neuroinflammation in a Mouse Model of Asthma [Pubmed: 31542455] |
| Europe PMC | Electrical stimulation of the superior sagittal sinus suppresses A-type K currents and increases P/Q-and T-type Ca 2 currents in rat trigeminal ganglion?… [Pubmed: 31375062] |
| Clinical Chemistry and Laboratory Medicine | Effects of endurance exercise on serum concentration of calcitonin gene-related peptide (CGRP): a potential link between exercise intensity and headache [Pubmed: 32286239] |
| MOLECULAR BIOLOGY REPORTS | Serum CGRP, VIP, and PACAP usefulness in migraine: a case–control study in chronic migraine patients in real clinical practice [Pubmed: 32951099] |
| Scientific Reports | Mild systemic inflammation enhances response to OnabotulinumtoxinA in chronic migraineurs [33441852] |
| Nature Communications | Perirenal adipose afferent nerves sustain pathological high blood pressure in rats [Pubmed:35668093] |
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