半胱氨酸蛋白酶抑制劑B(CSTB)檢測試劑盒DIY材料(酶聯免疫吸附試驗法)
ELISA Kit DIY Materials for Cystatin B (CSTB)
Cys-B; CST6; EPM1; PME; STFB; CPI-B; Stefin B; Liver thiol proteinase inhibitor
- 編號KSJ322Ra01
- 物種Rattus norvegicus (Rat,大鼠) 相同的名稱,不同的物種。
- 試劑內容 包被抗體、檢測抗體、標準品、HRP標記的鏈霉親和素、TMB底物、96孔板
- 可檢測樣本Serum, plasma, tissue homogenates and other biological fluids.
- 可適用原理雙抗夾心酶聯免疫吸附試驗法測抗原
- 可檢測范圍0.312-20ng/mL
- 可適靈敏度0.119ng/mL
- 應用Main materials for "Do It (ELISA Kit) Yourself".
- 下載 英文說明書 中文說明書
- 規格 96T*596T*10 96T*20 96T*50 96T*100
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特異性
試劑盒中的抗體對半胱氨酸蛋白酶抑制劑B(CSTB)具有高靈敏度和高特異性,經檢測與其它相似物質無明顯交叉反應。
用法
1.用100μL每孔的工作濃度的包被抗體包被96孔板,4度孵育過夜或者37度孵育2小時;
2.洗板1次;
3.用200μL每孔的工作濃度的封閉液,37度孵育1.5小時;
4.洗板1次。酶標板即可用于ELISA實驗,后續使用方法與ELISA同。
儲存
抗體,標準品和HRP標記的鏈霉親和素在-20度保存,TMB需在4度保存,96孔板在室溫保存。所有組分保質期為1年,試劑一旦開封,在4度保存時間為1個月。
配套試劑
增值服務
相關產品
編號 | 適用物種:Rattus norvegicus (Rat,大鼠) | 應用(僅供研究使用,不用于臨床診斷!) |
RPJ322Ra01 | 半胱氨酸蛋白酶抑制劑B(CSTB)重組蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
PAJ322Ra01 | 半胱氨酸蛋白酶抑制劑B(CSTB)多克隆抗體 | WB; IHC; ICC; IP. |
LAJ322Ra71 | 半胱氨酸蛋白酶抑制劑B(CSTB)多克隆抗體(生物素標記) | WB; IHC; ICC. |
MAJ322Ra24 | 半胱氨酸蛋白酶抑制劑B(CSTB)單克隆抗體 | WB |
MAJ322Ra26 | 半胱氨酸蛋白酶抑制劑B(CSTB)單克隆抗體 | WB; IHC; ICC; IP. |
MAJ322Ra22 | 半胱氨酸蛋白酶抑制劑B(CSTB)單克隆抗體 | WB; IHC; ICC; IP. |
MAJ322Ra21 | 半胱氨酸蛋白酶抑制劑B(CSTB)單克隆抗體 | WB; IHC; ICC; IP. |
LAJ322Ra74 | 半胱氨酸蛋白酶抑制劑B(CSTB)單克隆抗體(生物素標記) | WB; IHC; ICC. |
SEJ322Ra | 半胱氨酸蛋白酶抑制劑B(CSTB)檢測試劑盒(酶聯免疫吸附試驗法) | Enzyme-linked immunosorbent assay for Antigen Detection. |
LMJ322Ra | 半胱氨酸蛋白酶抑制劑B(CSTB)等多因子檢測試劑盒(流式熒光發光法) | FLIA Kit for Antigen Detection. |
PSJ322Ra01 | 半胱氨酸蛋白酶抑制劑B(CSTB)抗體對 | ELISA; CLIA; ELISPOT; Luminex; Immunochromatography and other Immunoassays. |
KSJ322Ra01 | 半胱氨酸蛋白酶抑制劑B(CSTB)檢測試劑盒DIY材料(酶聯免疫吸附試驗法) | Main materials for "Do It (ELISA Kit) Yourself". |
參考文獻
雜志 | 參考文獻 |
AIDS. | Cathepsin B and cystatin B in HIV-seropositive women are associated with infection and HIV-1-associated neurocognitive disorders [PubMed: 23291538] |
International Journal of Circumpolar Health | Evaluation of serum procathepsin B, cystatin B and cystatin C as possible biomarkers of ovarian cancer [PubMed: PMC3754495] |
PLoS One. | Dysregulation of Macrophage-Secreted Cathepsin B Contributes to HIV-1-Linked Neuronal Apoptosis [PubMed: PMC3365072] |
Journal of Neuroimmune Pharmacology | Cocaine potentiates cathepsin B secretion and neuronal apoptosis from HIV-infected macrophages [Pubmed:25209871] |
European Journal of Cancer Supplements | A91: Endogenous inhibitors of cysteine proteases and preform of cathepsin B in cancer of reproductive system [Science: Article] |
Annals of Clinical Biochemistry | Diagnostic specificity and sensitivity of PIVKAII, GP3, CSTB, SCCA1 and HGF for the diagnosis of hepatocellular carcinoma in patients with alcoholic liver cirrhosis. [pubmed:28766361] |
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