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饑餓素(GHRL)檢測試劑盒DIY材料(酶聯免疫吸附試驗法)

ELISA Kit DIY Materials for Ghrelin (GHRL)

MTLRP; Growth Hormone-Releasing Peptide

  • 編號KSA991Mu11
  • 物種Mus musculus (Mouse,小鼠) 相同的名稱,不同的物種。
  • 試劑內容 包被抗體、生物素標記的競爭物、標準品、HRP標記的鏈霉親和素、TMB底物、96孔板
  • 可檢測樣本Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids.
  • 可適用原理競爭抑制酶聯免疫吸附試驗法測抗原
  • 可檢測范圍123.46-10000pg/mL
  • 可適靈敏度51.3pg/mL
  • 應用Main materials for "Do It (ELISA Kit) Yourself".
  • 下載 英文說明書   中文說明書
  • 規格 96T*596T*10 96T*20 96T*50 96T*100
  • 價格 ¥ 6998 ¥ 11664 ¥ 20995 ¥ 40824 ¥ 69984
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  • 饑餓素(GHRL)檢測試劑盒DIY材料(酶聯免疫吸附試驗法) 產品包裝(模擬)
  • 饑餓素(GHRL)檢測試劑盒DIY材料(酶聯免疫吸附試驗法) 產品包裝(模擬)
  • Certificate 通過ISO 9001、ISO 13485質量體系認證

特異性

試劑盒中的抗體對饑餓素(GHRL)具有高靈敏度和高特異性,經檢測與其它相似物質無明顯交叉反應。

用法

1.用100μL每孔的工作濃度的包被抗體包被96孔板,4度孵育過夜或者37度孵育2小時;
2.洗板1次;
3.用200μL每孔的工作濃度的封閉液,37度孵育1.5小時;
4.洗板1次。酶標板即可用于ELISA實驗,后續使用方法與ELISA同。

儲存

抗體,標準品和HRP標記的鏈霉親和素在-20度保存,TMB需在4度保存,96孔板在室溫保存。所有組分保質期為1年,試劑一旦開封,在4度保存時間為1個月。

配套試劑

相關產品

編號 適用物種:Mus musculus (Mouse,小鼠) 應用(僅供研究使用,不用于臨床診斷!)
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參考文獻

雜志 參考文獻
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Department of Medical Biochemistry and Clinical Biochemistry Presence of adropin, nesfatin-1, apelin-12, ghrelins and salusins peptides in the milk, cheese whey and plasma of dairy cows [ScienceDirect: S0196978113000533]
Journal of Physiology and Pharmacology Postnatal administration of 2-oxoglutaric acid improves articular and growth plate cartilages and bone tissue morphology in pigs prenatally treated with dexamethasone [PubMed: 23211309]
Gene The modifying effects of fish oil on fasting ghrelin mRNA expression in weaned rats [PubMed: 22842192]
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Surg Innov Gastric Supply Manipulation to Modulate Ghrelin Production and Enhance Vascularization to the Cardia Proof of the Concept in a Porcine Model [PubMed: 25294792]
Nutrients.? Elevation of Fasting Ghrelin in Healthy Human Subjects Consuming a High-Salt Diet: A Novel Mechanism of Obesity? [Pubmed:27240398]
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Ginekologia?Polska Increased?serum?ghrelin?in?preeclampsia: is?ghrelin?a?friend?or a foe? [pubmed:27321099]
Gynecological Endocrinology The association between serum ghrelin levels and large-for-gestational-age fetuses in patients with gestational diabetes mellitus [pubmed:27791436]
Acta Diabetologica Effect of lifestyle improvement program on the biomarkersof adiposity, inflammation and gut hormones in overweight/obese Asian Indians with prediabetes [pubmed:28620678]
Scandinavian Journal of Clinical and Laboratory Investigation Role of serotonin hormone in weight regain after sleeve gastrectomy [pubmed:29228802]
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American Journal of Perinatology The Role of Umbilical Cord Blood Concentration of IGF-I, IGF-II, Leptin, Adiponectin, Ghrelin, Resistin, and Visfatin in Fetal Growth [Pubmed: 30282106]
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The Journal of Nutrition Grape Seed Proanthocyanidin Affects Lipid Metabolism via Changing Gut Microflora and Enhancing Propionate Production in Weaned Pigs [Pubmed: 31175811]
Arch Physiol Biochem Associations of apelin, leptin, irisin, ghrelin, insulin, glucose levels, and lipid parameters with physical activity during eight weeks of regular exercise training [Pubmed: 31290696]
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