白介素15(IL15)檢測試劑盒DIY材料(酶聯免疫吸附試驗法)
ELISA Kit DIY Materials for Interleukin 15 (IL15)
- 編號KSA061Ga01
- 物種Chicken (Gallus,雞) 相同的名稱,不同的物種。
- 試劑內容 包被抗體、檢測抗體、標準品、HRP標記的鏈霉親和素、TMB底物、96孔板
- 可檢測樣本Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
- 可適用原理雙抗夾心酶聯免疫吸附試驗法測抗原
- 可檢測范圍15.6-1,000pg/mL
- 可適靈敏度6.2pg/mL
- 應用Main materials for "Do It(ELISA Kit) Yourself"
- 下載 英文說明書 中文說明書
- 規格 96T*596T*10 96T*20 96T*50 96T*100
- 價格 ¥ 8168 ¥ 13614 ¥ 24505 ¥ 47649 ¥ 81684
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特異性
試劑盒中的抗體對白介素15(IL15)具有高靈敏度和高特異性,經檢測與其它相似物質無明顯交叉反應。
用法
1.用100μL每孔的工作濃度的包被抗體包被96孔板,4度孵育過夜或者37度孵育2小時;
2.洗板1次;
3.用200μL每孔的工作濃度的封閉液,37度孵育1.5小時;
4.洗板1次。酶標板即可用于ELISA實驗,后續使用方法與ELISA同。
儲存
抗體,標準品和HRP標記的鏈霉親和素在-20度保存,TMB需在4度保存,96孔板在室溫保存。所有組分保質期為1年,試劑一旦開封,在4度保存時間為1個月。
配套試劑
增值服務
相關產品
編號 | 適用物種:Chicken (Gallus,雞) | 應用(僅供研究使用,不用于臨床診斷!) |
RPA061Ga01 | 白介素15(IL15)重組蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
PAA061Ga01 | 白介素15(IL15)多克隆抗體 | WB; IHC; ICC; IP. |
SEA061Ga | 白介素15(IL15)檢測試劑盒(酶聯免疫吸附試驗法) | Enzyme-linked immunosorbent assay for Antigen Detection. |
LMA061Ga | 白介素15(IL15)等多因子檢測試劑盒(流式熒光發光法) | FLIA Kit for Antigen Detection. |
KSA061Ga01 | 白介素15(IL15)檢測試劑盒DIY材料(酶聯免疫吸附試驗法) | Main materials for "Do It(ELISA Kit) Yourself" |
參考文獻
雜志 | 參考文獻 |
Endocrine | Expression of interleukin-15 and inflammatory cytokines in skeletal muscles of STZ-induced diabetic rats: effect of resistance exercise training [Pubmed: 24006180] |
Endocrine | Time course of IL-15 expression after acute resistance exercise in trained rats: effect of diabetes and skeletal muscle phenotype [Pubmed:25522723] |
Med Sci Moni | miR-15b is Downregulated in Myasthenia Gravis Patients and Directly Regulates the Expression of Interleukin-15 (IL-15) in Experimental Myasthenia Gravis Mice [PubMed: 26087886] |
Exercise physiology | Effects of intrinsic aerobic capacity, aging and physical activity on interleukin-15 protein level in serum and skeletal muscle [Dspace: Handle] |
Biotechnology Letters | Novel adjuvant for immunization against tuberculosis: DNA vaccine expressing Mycobacterium tuberculosis antigen 85A and interleukin-15 fusion product elicits strong immune responses in mice [s10529-017-2342-1] |
Journal?of?Controlled?Release | Interleukin-15 and cisplatin co-encapsulated thermosensitive polypeptide hydrogels for combined immuno-chemotherapy. [pubmed:28408199] |
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