溶血磷脂酰膽堿(LPC)檢測試劑盒DIY材料(酶聯免疫吸附試驗法)
ELISA Kit DIY Materials for Lysophosphatidylcholine (LPC)
lysoPC; Lysolecithin; 1-Palmitoyl-sn-Glycero-3-Phosphocholine
- 編號KSK621Ge11
- 物種Pan-species (General，通用) 相同的名稱，不同的物種。
- 試劑內容 包被抗體、生物素標記的競爭物、標準品、HRP標記的鏈霉親和素、TMB底物、96孔板
- 可檢測樣本Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
- 可適用原理競爭抑制酶聯免疫吸附試驗法測抗原
- 可檢測范圍246.9-20,000ng/mL
- 可適靈敏度89.3ng/mL
- 應用Main materials for "Do It (ELISA Kit) Yourself".
- 下載 英文說明書 中文說明書
- 規格 96T*596T*10 96T*20 96T*50 96T*100
- 價格 ¥ 8813 ¥ 14688 ¥ 26438 ¥ 51408 ¥ 88128
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產品包裝(模擬)
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產品包裝(模擬)
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通過ISO 9001、ISO 13485質量體系認證
特異性
試劑盒中的抗體對溶血磷脂酰膽堿(LPC)具有高靈敏度和高特異性，經檢測與其它相似物質無明顯交叉反應。
用法
1.用100μL每孔的工作濃度的包被抗體包被96孔板，4度孵育過夜或者37度孵育2小時；
2.洗板1次；
3.用200μL每孔的工作濃度的封閉液,37度孵育1.5小時；
4.洗板1次。酶標板即可用于ELISA實驗，后續使用方法與ELISA同。
儲存
抗體，標準品和HRP標記的鏈霉親和素在-20度保存，TMB需在4度保存，96孔板在室溫保存。所有組分保質期為1年，試劑一旦開封，在4度保存時間為1個月。
配套試劑
增值服務
相關產品
| 編號 | 適用物種：Pan-species (General，通用) | 應用(僅供研究使用，不用于臨床診斷！) |
| CPK621Ge11 | 溶血磷脂酰膽堿(LPC)牛血清白蛋白偶聯物 | Immunogen; SDS-PAGE; WB. |
| CPK621Ge21 | 溶血磷脂酰膽堿(LPC)卵白蛋白偶聯物 | Immunogen; SDS-PAGE; WB. |
| PAK621Ge01 | 溶血磷脂酰膽堿(LPC)多克隆抗體 | ELISA, CLIA. / IHC-Fr, ICC, IP (predicted). |
| CEK621Ge | 溶血磷脂酰膽堿(LPC)檢測試劑盒(酶聯免疫吸附試驗法) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| LMK621Ge | 溶血磷脂酰膽堿(LPC)等多因子檢測試劑盒(流式熒光發光法) | FLIA Kit for Antigen Detection. |
| KSK621Ge11 | 溶血磷脂酰膽堿(LPC)檢測試劑盒DIY材料(酶聯免疫吸附試驗法) | Main materials for "Do It (ELISA Kit) Yourself". |
參考文獻
| 雜志 | 參考文獻 |
| Scientific Reports | High-fat diet-induced acceleration of osteoarthritis is associated with a distinct and sustained plasma metabolite signature [pubmed:28811491] |
| Stem?Cells | Iron Homeostasis Determines Fate of Human Pluripotent Stem Cells Via Glycerophospholipids‐Epigenetic Circuit [Pubmed: 30599084] |
| Lipids?in?Health?and Disease | Associations between plasma lysophospholipids concentrations, chronic kidney disease and the type of renal replacement therapy [Pubmed: 30947711] |
| Nature Communications | ER-residential Nogo-B accelerates NAFLD-associated HCC mediated by metabolic reprogramming of oxLDL lipophagy [Pubmed: 31358770] |
| NUTRIENTS | Dietary Betaine Addition Promotes Hepatic Cholesterol Synthesis, Bile Acid Conversion, and Export in Rats [Pubmed: 32414094] |
| Cells | iPLA2|? Contributes to ER Stress-Induced Apoptosis during Myocardial Ischemia/Reperfusion Injury [] |
| European Journal of Pharmacology | Downregulation of activating transcription factor 4 attenuates lysophosphatidycholine-induced inflammation via the NF-κB pathway [34582847] |
| Oxid Med Cell Longev | Astragaloside IV Inhibits Bleomycin-Induced Ferroptosis in Human Umbilical Vein Endothelial Cells by Mediating LPC [34760046] |
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