線粒體開放閱讀框12S rRNA-C(MOTS-c)檢測試劑盒DIY材料(酶聯免疫吸附試驗法)
ELISA Kit DIY Materials for Mitochondrial Open Reading Frame Of The 12S rRNA-c (MOTS-c)
- 編號KSX132Hu11
- 物種Homo sapiens (Human，人) 相同的名稱，不同的物種。
- 試劑內容 包被抗體、生物素標記的競爭物、標準品、HRP標記的鏈霉親和素、TMB底物、96孔板
- 可檢測樣本serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
- 可適用原理競爭抑制酶聯免疫吸附試驗法測抗原
- 可檢測范圍4.94-400ng/mL
- 可適靈敏度
- 應用Main materials for "Do It (ELISA Kit) Yourself".
- 下載 英文說明書 中文說明書
- 規格 96T*596T*10 96T*20 96T*50 96T*100
- 價格 ¥ 8316 ¥ 13860 ¥ 24948 ¥ 48510 ¥ 83160
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產品包裝(模擬)
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產品圖片
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通過ISO 9001、ISO 13485質量體系認證
特異性
試劑盒中的抗體對線粒體開放閱讀框12S rRNA-C(MOTS-c)具有高靈敏度和高特異性，經檢測與其它相似物質無明顯交叉反應。
用法
1.用100μL每孔的工作濃度的包被抗體包被96孔板，4度孵育過夜或者37度孵育2小時；
2.洗板1次；
3.用200μL每孔的工作濃度的封閉液,37度孵育1.5小時；
4.洗板1次。酶標板即可用于ELISA實驗，后續使用方法與ELISA同。
儲存
抗體，標準品和HRP標記的鏈霉親和素在-20度保存，TMB需在4度保存，96孔板在室溫保存。所有組分保質期為1年，試劑一旦開封，在4度保存時間為1個月。
配套試劑
增值服務
相關產品
| 編號 | 適用物種：Homo sapiens (Human，人) | 應用(僅供研究使用，不用于臨床診斷！) |
| SPX132Hu01 | 線粒體開放閱讀框12S rRNA-C(MOTS-c)合成多肽 | Immunogen; Blocking Peptide. |
| CPX132Hu21 | 線粒體開放閱讀框12S rRNA-C(MOTS-c)卵白蛋白偶聯物 | Immunogen; SDS-PAGE; WB. |
| CPX132Hu11 | 線粒體開放閱讀框12S rRNA-C(MOTS-c)牛血清白蛋白偶聯物 | Immunogen; SDS-PAGE; WB. |
| PAX132Hu01 | 線粒體開放閱讀框12S rRNA-C(MOTS-c)多克隆抗體 | IHC; ICC/IF; IP |
| CEX132Hu | 線粒體開放閱讀框12S rRNA-C(MOTS-c)檢測試劑盒(酶聯免疫吸附試驗法) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| MEX132Hu | 線粒體開放閱讀框12S rRNA-C(MOTS-c)檢測試劑盒(酶聯免疫吸附試驗法,小樣本) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| LMX132Hu | 線粒體開放閱讀框12S rRNA-C(MOTS-c)等多因子檢測試劑盒(流式熒光發光法) | FLIA Kit for Antigen Detection. |
| KSX132Hu11 | 線粒體開放閱讀框12S rRNA-C(MOTS-c)檢測試劑盒DIY材料(酶聯免疫吸附試驗法) | Main materials for "Do It (ELISA Kit) Yourself". |
參考文獻
| 雜志 | 參考文獻 |
| Pediatric?Diabetes? | Circulating MOTS‐c levels are decreased in obese male children and adolescents and associated with insulin resistance [Pubmed:29691953] |
| Rapid?Commun?Mass?Spectrom | Development of a mass spectrometry‐based detection method for the mitochondrion‐derived peptide MOTS‐c in plasma samples for doping control purposes [Pubmed: 30394592] |
| DIABETOLOGIA | Adiponectin treatment improves insulin resistance in mice by regulating the expression of the mitochondrial-derived peptide MOTS-c and its response to exercise via?… [Pubmed: 32880686] |
| Induction of hyperandrogenism and insulin resistance differentially modulates ferroptosis in uterine and placental tissues of pregnant rats [] | |
| FASEB JOURNAL | Neural cell?\derived plasma exosome protein abnormalities implicate mitochondrial impairment in first episodes of psychosis [33454965] |
| Biochim Biophys Acta Mol Basis Dis | MOTS-c interacts synergistically with exercise intervention to regulate PGC-1|á expression, attenuate insulin resistance and enhance glucose metabolism in mice via??- [33722744] |
| researchsquare | Evolution of Mitochondrial-Derived Peptides, Humanin and MOTSc and Changes in Insulin Sensitivity During Early Gestation [] |
| Mol Psychiatry | Abnormal levels of mitochondrial proteins in plasma neuronal extracellular vesicles in major depressive disorder [34471251] |
| Pharmacol Res | The Mitochondrial-Derived Peptide MOTS-c Relieves Hyperglycemia and Insulin Resistance in Gestational Diabetes Mellitus [34798268] |
| Ann Neurol | SARS‐CoV‐2 and mitochondrial proteins in neural‐derived exosomes of COVID‐19 [Pubmed:35285072] |
| J Clin Med | Evolution of Mitochondrially Derived Peptides Humanin and MOTSc, and Changes in Insulin Sensitivity during Early Gestation in Women with and without Gestational?… [Pubmed:35683389] |
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