粘蛋白5B(MUC5B)檢測試劑盒DIY材料(酶聯免疫吸附試驗法)
ELISA Kit DIY Materials for Mucin 5 Subtype B (MUC5B)
MUC5-B; MUC9; MG1; Oligomeric Mucus/Gel-Forming; Mucin 5,Subtype B,Tracheobronchial; Cervical mucin; High molecular weight salivary mucin MG1; Sublingual gland mucin
- 編號KSA684Ra01
- 物種Rattus norvegicus (Rat，大鼠) 相同的名稱，不同的物種。
- 試劑內容 包被抗體、檢測抗體、標準品、HRP標記的鏈霉親和素、TMB底物、96孔板
- 可檢測樣本Tissue homogenates, cell lysates, cell culture supernates and other biological fluids.
- 可適用原理雙抗夾心酶聯免疫吸附試驗法測抗原
- 可檢測范圍0.312-20ng/mL
- 可適靈敏度0.115ng/mL
- 應用Main materials for "Do It (ELISA Kit) Yourself".
- 下載 英文說明書 中文說明書
- 規格 96T*596T*10 96T*20 96T*50 96T*100
- 價格 ¥ 7387 ¥ 12312 ¥ 22162 ¥ 43092 ¥ 73872
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產品包裝(模擬)
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產品包裝(模擬)
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通過ISO 9001、ISO 13485質量體系認證
特異性
試劑盒中的抗體對粘蛋白5B(MUC5B)具有高靈敏度和高特異性，經檢測與其它相似物質無明顯交叉反應。
用法
1.用100μL每孔的工作濃度的包被抗體包被96孔板，4度孵育過夜或者37度孵育2小時；
2.洗板1次；
3.用200μL每孔的工作濃度的封閉液,37度孵育1.5小時；
4.洗板1次。酶標板即可用于ELISA實驗，后續使用方法與ELISA同。
儲存
抗體，標準品和HRP標記的鏈霉親和素在-20度保存，TMB需在4度保存，96孔板在室溫保存。所有組分保質期為1年，試劑一旦開封，在4度保存時間為1個月。
配套試劑
增值服務
相關產品
| 編號 | 適用物種：Rattus norvegicus (Rat，大鼠) | 應用(僅供研究使用，不用于臨床診斷！) |
| RPA684Ra02 | 粘蛋白5B(MUC5B)重組蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| RPA684Ra03 | 粘蛋白5B(MUC5B)重組蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| RPA684Ra01 | 粘蛋白5B(MUC5B)重組蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| PAA684Ra03 | 粘蛋白5B(MUC5B)多克隆抗體 | WB; IHC; ICC; IP. |
| PAA684Ra02 | 粘蛋白5B(MUC5B)多克隆抗體 | WB; IHC; ICC; IP. |
| PAA684Ra01 | 粘蛋白5B(MUC5B)多克隆抗體 | WB; IHC; ICC; IP. |
| SEA684Ra | 粘蛋白5B(MUC5B)檢測試劑盒(酶聯免疫吸附試驗法) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| LMA684Ra | 粘蛋白5B(MUC5B)等多因子檢測試劑盒(流式熒光發光法) | FLIA Kit for Antigen Detection. |
| KSA684Ra01 | 粘蛋白5B(MUC5B)檢測試劑盒DIY材料(酶聯免疫吸附試驗法) | Main materials for "Do It (ELISA Kit) Yourself". |
參考文獻
| 雜志 | 參考文獻 |
| The Journal of Biological Chemistry | The Extracellular Signal-regulated Kinase Mitogen-activated Protein Kinase/Ribosomal S6 Protein Kinase 1 Cascade Phosphorylates cAMP Response Element-binding Protein to Induce MUC5B Gene Expression via D-Prostanoid Receptor Signaling [Jcb: 34199] |
| The Journal of Biological Chemistry | The ERK MAPK/RSK1 cascade phosphorylates CREB to induce MUC5B gene expression via DP1 receptor signaling [Jcb: 247684] |
| Respiratory Researh | Pyocyanin-induced mucin production is associated with redox modification of FOXA2 [PubMed: PMC3765780] |
| Respirology | Secreted mucins and airway bacterial colonization in non-CF bronchiectasis [PubMed: 26172851] |
| American Thoracic Society International Conference Abstracts | Mucin Secretion In Asthmatics With Osmotic Airway Hyperresponsiveness [doi:10.1164] |
| Ann Am Thorac Soc | Airway Mucin 2 Is Decreased in Patients with Severe Chronic Obstructive Pulmonary Disease with Bacterial Colonization [doi:10.1513] |
| Секреторно-экссудативная реакция слизистой носа на воздействие холодного воздуха у больных бронхиальной астмой [10.20538/1682-0363-2017-2-146-158] | |
| Allergy | Th2 cytokines orchestrate the secretion of MUC 5 AC and MUC 5B in IL‐5 positive chronic rhinosinusitis with nasal polyps [Pubmed:29802623] |
| European?Journal of Pharmacology? | Salvianolic acid B improves airway hyperresponsiveness by inhibiting MUC5AC overproduction associated with Erk1/2/P38 signaling [Pubmed:29382534] |
| Japan Society of Immunology & Allergology in Otolaryngology | インフルエンザ菌Phosphorylcholine の表出と中耳ムチンMUC5AC 及びMUC5B 産生への影響 [10.5648:jjiao.36.7] |
| Journal of Investigative?Medicine | The characteristics and clinical significance of mucin levels in bronchoalveolar lavage fluid of patients with interstitial lung disease [Pubmed: 30573494] |
| Mucosal Immunology | ASC and NLRP3 maintain innate immune homeostasis in the airway through an inflammasome-independent mechanism [Pubmed: 31278375] |
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