P物質(SP)檢測試劑盒DIY材料(酶聯免疫吸附試驗法)
ELISA Kit DIY Materials for Substance P (SP)
- 編號KSA393Ra11
- 物種Rattus norvegicus (Rat,大鼠) 相同的名稱,不同的物種。
- 試劑內容 包被抗體、生物素標記的競爭物、標準品、HRP標記的鏈霉親和素、TMB底物、96孔板
- 可檢測樣本serum, plasma and other biological fluids
- 可適用原理競爭抑制酶聯免疫吸附試驗法測抗原
- 可檢測范圍24.69-2,000pg/mL
- 可適靈敏度
- 應用Main materials for "Do It (ELISA Kit) Yourself".
- 下載 英文說明書 中文說明書
- 規格 96T*596T*10 96T*20 96T*50 96T*100
- 價格 ¥ 7387 ¥ 12312 ¥ 22162 ¥ 43092 ¥ 73872
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特異性
試劑盒中的抗體對P物質(SP)具有高靈敏度和高特異性,經檢測與其它相似物質無明顯交叉反應。
用法
1.用100μL每孔的工作濃度的包被抗體包被96孔板,4度孵育過夜或者37度孵育2小時;
2.洗板1次;
3.用200μL每孔的工作濃度的封閉液,37度孵育1.5小時;
4.洗板1次。酶標板即可用于ELISA實驗,后續使用方法與ELISA同。
儲存
抗體,標準品和HRP標記的鏈霉親和素在-20度保存,TMB需在4度保存,96孔板在室溫保存。所有組分保質期為1年,試劑一旦開封,在4度保存時間為1個月。
配套試劑
增值服務
相關產品
編號 | 適用物種:Rattus norvegicus (Rat,大鼠) | 應用(僅供研究使用,不用于臨床診斷!) |
CPA393Ra22 | P物質(SP)卵白蛋白偶聯物 | Immunogen; SDS-PAGE; WB. |
PAA393Ra01 | P物質(SP)多克隆抗體 | WB; IHC; ICC; IP. |
CEA393Ra | P物質(SP)檢測試劑盒(酶聯免疫吸附試驗法) | Enzyme-linked immunosorbent assay for Antigen Detection. |
CCA393Ra | P物質(SP)檢測試劑盒(化學發光免疫分析法) | Chemiluminescent immunoassay for Antigen Detection. |
LMA393Ra | P物質(SP)等多因子檢測試劑盒(流式熒光發光法) | FLIA Kit for Antigen Detection. |
KSA393Ra11 | P物質(SP)檢測試劑盒DIY材料(酶聯免疫吸附試驗法) | Main materials for "Do It (ELISA Kit) Yourself". |
參考文獻
雜志 | 參考文獻 |
Journal of Veterinary Internal Medicine | A Potential Role for Substance P and Interleukin-6 in the Cerebrospinal Fluid of Cavalier King Charles Spaniels with Neuropathic Pain [Pubmed: 23659719] |
Pharmacol Res | Oral curcumin has anti-arthritic efficacy through somatostatin generation [PubMed: 25836921] |
Applied Biological Chemistry | Positive enhancement of Lactobacillus fermentum HY01 on intestinal movements of mice having constipation [10.1007/s13765-017-0327-3] |
CNS?Neuroscience & Therapeutics? | Direct activation of tachykinin receptors within baroreflex afferent pathway and neurocontrol of blood pressure regulation [Pubmed:29900692] |
Journal of Veterinary Science | Serum Levels of Neuropeptides in Cows with Left Abomasal Displacement [Pubmed: 30562932] |
Respiratory Physiology & Neurobiology | Effects of nanoparticles on Neuroinflammation in a Mouse Model of Asthma [Pubmed: 31542455] |
Chinese Medicine | Hejie Zhitong prescription promotes sleep and inhibits nociceptive transmission-associated neurotransmitter activity in a rodent migraine model [Pubmed: 33014123] |
Medical Research Journal | Changes of substance P, NGF and CGRP salivary levels among patients undergoing physical therapy [] |
Int Urol Nephrol | Evaluation of the causes affecting the development of pruritus in patients with peritoneal dialysis [34213714] |
J Chem Neuroanat | Surfactant protein C is associated with perineuronal nets and shows age-dependent changes of brain content and hippocampal deposits in wildtype and 3xTg mice [34626771] |
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