線粒體超氧化物歧化酶(SOD2)檢測試劑盒DIY材料(酶聯免疫吸附試驗法)
ELISA Kit DIY Materials for Superoxide Dismutase 2, Mitochondrial (SOD2)
IPO-B; MNSOD; Mn-SOD
- 編號KSB083Ra01
- 物種Rattus norvegicus (Rat，大鼠) 相同的名稱，不同的物種。
- 試劑內容 包被抗體、檢測抗體、標準品、HRP標記的鏈霉親和素、TMB底物、96孔板
- 可檢測樣本Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids.
- 可適用原理雙抗夾心酶聯免疫吸附試驗法測抗原
- 可檢測范圍31.2-2000pg/mL
- 可適靈敏度14.5pg/mL
- 應用自己動手制作ELISA試劑盒的主要材料
- 下載 英文說明書 中文說明書
- 規格 96T*596T*10 96T*20 96T*50 96T*100
- 價格 ¥ 7798 ¥ 12996 ¥ 23393 ¥ 45486 ¥ 77976
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產品包裝(模擬)
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產品包裝(模擬)
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通過ISO 9001、ISO 13485質量體系認證
特異性
試劑盒中的抗體對線粒體超氧化物歧化酶(SOD2)具有高靈敏度和高特異性，經檢測與其它相似物質無明顯交叉反應。
用法
1.用100μL每孔的工作濃度的包被抗體包被96孔板，4度孵育過夜或者37度孵育2小時；
2.洗板1次；
3.用200μL每孔的工作濃度的封閉液,37度孵育1.5小時；
4.洗板1次。酶標板即可用于ELISA實驗，后續使用方法與ELISA同。
儲存
抗體，標準品和HRP標記的鏈霉親和素在-20度保存，TMB需在4度保存，96孔板在室溫保存。所有組分保質期為1年，試劑一旦開封，在4度保存時間為1個月。
配套試劑
增值服務
相關產品
| 編號 | 適用物種：Rattus norvegicus (Rat，大鼠) | 應用(僅供研究使用，不用于臨床診斷！) |
| APB083Ra01 | 線粒體超氧化物歧化酶(SOD2)活性蛋白 | Cell?culture;?Activity?Assays. |
| RPB083Ra01 | 線粒體超氧化物歧化酶(SOD2)重組蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| PAB083Ra01 | 線粒體超氧化物歧化酶(SOD2)多克隆抗體 | WB,IHC,ICC/IF |
| LAB083Ra71 | 線粒體超氧化物歧化酶(SOD2)多克隆抗體(生物素標記) | WB; IHC; ICC. |
| LAB083Ra81 | 線粒體超氧化物歧化酶(SOD2)多克隆抗體(異硫氰酸熒光素標記) | WB; IHC; ICC; IF. |
| MAB083Ra21 | 線粒體超氧化物歧化酶(SOD2)單克隆抗體 | WB; IHC; ICC; IP. |
| SEB083Ra | 線粒體超氧化物歧化酶(SOD2)檢測試劑盒(酶聯免疫吸附試驗法) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| LMB083Ra | 線粒體超氧化物歧化酶(SOD2)等多因子檢測試劑盒(流式熒光發光法) | FLIA Kit for Antigen Detection. |
| KSB083Ra01 | 線粒體超氧化物歧化酶(SOD2)檢測試劑盒DIY材料(酶聯免疫吸附試驗法) | 自己動手制作ELISA試劑盒的主要材料 |
參考文獻
| 雜志 | 參考文獻 |
| Oral Disease | Antioxidant profile of salivary glands in high fat diet-induced insulin resistance rats [Pubmed: 24106991] |
| AGE | Consequences of age on ischemic wound healing in rats: altered antioxidant activity and delayed wound closure [Pubmed:24443098] |
| Anticancer Research | Determination of Gene Expression and Serum Levels of MnSOD and GPX1 in Colorectal Cancer [Pubmed:25550558] |
| Arch Oral Biol | Antioxidant profile, carbonyl and lipid oxidation markers in the parotid and submandibular glands of rats in different periods of streptozotocin induced diabetes [PubMed: 26143097] |
| J Oral Pathol Med | Impact of morbid obesity and bariatric surgery on antioxidant/oxidant balance of the unstimulated and stimulated human saliva [PubMed: 26608886] |
| Free Radic Biol Med. | DDAH1 deficiency promotes intracellular oxidative stress and cell apoptosis via a miR-21-dependent pathway in mouse embryonic fibroblasts. [Pubmed:26806551] |
| Basic?Research?in Cardiology | Cardiomyocyte dimethylarginine dimethylaminohydrolase1 attenuates left-ventricular remodeling after acute myocardial infarction: involvement in oxidative stress and?… [Pubmed:29892894] |
| Journal of Pharmacological?Sciences | Improved endogenous epoxyeicosatrienoic acid production mends heart function via increased PGC 1α-mitochondrial functions in metabolic syndrome [Pubmed: 30342783] |
| Advances in Medical Sciences | Selected elements of extracellular matrix of the skin in diabetes and insulin resistance [Pubmed: 31146169] |
| PHARMACOLOGICAL RESEARCH | A Machine Learning-driven Study Indicates Emodin Improves Cardiac Hypertrophy by Modulation of Mitochondrial SIRT3 Signaling [Pubmed: 32135248] |
| Biomolecules | Arachidonic Acid as an Early Indicator of Inflammation during Non-Alcoholic Fatty Liver Disease Development [Pubmed: 32751983] |
| Nutrients | Attenuation of Oxidative Stress and Inflammatory Response by Chronic Cannabidiol Administration Is Associated with Improved n-6/n-3 PUFA Ratio in the White and??- [34064937] |
| Pharmaceuticals | Serum Total SOD Activity and SOD1/2 Concentrations in Predicting All-Cause Mortality in Lung Cancer Patients [34832849] |
| Biochimica et Biophysica Acta-Molecular Basis of Disease | α-Lipoic acid ameliorates inflammation state and oxidative stress by reducing the content of bioactive lipid derivatives in the left ventricle of rats fed a high-fat diet [Pubmed:35569738] |
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