多配體蛋白聚糖1(SDC1)檢測試劑盒DIY材料(酶聯免疫吸附試驗法)
ELISA Kit DIY Materials for Syndecan 1 (SDC1)
CD138; SDC; SYND1; Syndecan Proteoglycan 1
- 編號KSB966Ra01
- 物種Rattus norvegicus (Rat，大鼠) 相同的名稱，不同的物種。
- 試劑內容 包被抗體、檢測抗體、標準品、HRP標記的鏈霉親和素、TMB底物、96孔板
- 可檢測樣本Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
- 可適用原理雙抗夾心酶聯免疫吸附試驗法測抗原
- 可檢測范圍1.56-100ng/mL
- 可適靈敏度0.67ng/m
- 應用自己動手制作ELISA試劑盒的主要材料
- 下載 英文說明書 中文說明書
- 規格 96T*596T*10 96T*20 96T*50 96T*100
- 價格 ¥ 7798 ¥ 12996 ¥ 23393 ¥ 45486 ¥ 77976
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產品包裝(模擬)
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產品包裝(模擬)
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通過ISO 9001、ISO 13485質量體系認證
特異性
試劑盒中的抗體對多配體蛋白聚糖1(SDC1)具有高靈敏度和高特異性，經檢測與其它相似物質無明顯交叉反應。
用法
1.用100μL每孔的工作濃度的包被抗體包被96孔板，4度孵育過夜或者37度孵育2小時；
2.洗板1次；
3.用200μL每孔的工作濃度的封閉液,37度孵育1.5小時；
4.洗板1次。酶標板即可用于ELISA實驗，后續使用方法與ELISA同。
儲存
抗體，標準品和HRP標記的鏈霉親和素在-20度保存，TMB需在4度保存，96孔板在室溫保存。所有組分保質期為1年，試劑一旦開封，在4度保存時間為1個月。
配套試劑
增值服務
相關產品
| 編號 | 適用物種：Rattus norvegicus (Rat，大鼠) | 應用(僅供研究使用，不用于臨床診斷！) |
| RPB966Ra02 | 多配體蛋白聚糖1(SDC1)重組蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| RPB966Ra01 | 多配體蛋白聚糖1(SDC1)重組蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| PAB966Ra02 | 多配體蛋白聚糖1(SDC1)多克隆抗體 | WB; IHC; ICC; IP. |
| PAB966Ra01 | 多配體蛋白聚糖1(SDC1)多克隆抗體 | WB; IHC; ICC; IP. |
| LAB966Ra81 | 多配體蛋白聚糖1(SDC1)多克隆抗體(異硫氰酸熒光素標記) | WB; IHC; ICC; IF. |
| MAB966Ra21 | 多配體蛋白聚糖1(SDC1)單克隆抗體 | WB; IHC; ICC; IP. |
| SEB966Ra | 多配體蛋白聚糖1(SDC1)檢測試劑盒(酶聯免疫吸附試驗法) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| LMB966Ra | 多配體蛋白聚糖1(SDC1)等多因子檢測試劑盒(流式熒光發光法) | FLIA Kit for Antigen Detection. |
| KSB966Ra01 | 多配體蛋白聚糖1(SDC1)檢測試劑盒DIY材料(酶聯免疫吸附試驗法) | 自己動手制作ELISA試劑盒的主要材料 |
參考文獻
| 雜志 | 參考文獻 |
| Molecular and Cellular Biochemistry | Increased level of soluble syndecan-1 in serum correlates with myocardial expression in a rat model of myocardial infarction [SpringerLink: a201w3l18k257462] |
| Inflammation | Sdc1 Overexpression Inhibits the p38 MAPK Pathway and Lessens Fibrotic Ventricular Remodeling in MI Rats [Springer: Source] |
| Atherosclerosis. | Damage of the endothelial glycocalyx in chronic kidney disease [Pubmed:24727235] |
| Journal of Trauma and Acute Care Surgery | Effect of valproic acid and injury on lesion size and endothelial glycocalyx shedding in a rodent model of isolated traumatic brain injury [Pubmed:25058256] |
| Shock | Impairment of the Endothelial Glycocalyx in Cardiogenic Shock and its Prognostic Relevance [Pubmed:25692257] |
| Mediators Inflamm | Dexamethasone Suppressed LPS-Induced Matrix Metalloproteinase and Its Effect on Endothelial Glycocalyx Shedding [PubMed: 26199464] |
| Transplantation | Platelet-Derived Growth Factor-B Protects Rat Cardiac Allografts From Ischemia-reperfusion Injury. [PubMed: 26371596] |
| Clinical and Experimental Nephrology | Dual antiplatelet and anticoagulant APAC prevents experimental ischemia–reperfusion-induced acute kidney injury [pubmed:27405618] |
| Biomarkers in Medicine | Endothelial glycocalyx layer shedding following lung resection [pubmed:27643669] |
| Danish National RESEARCH DATABASE | Resuscitation with Pooled and Pathogen-Reduced Plasma Attenuates the Increase in Brain Water Content following Traumatic Brain Injury and Hemorrhagic Shock in Rats [10.1089] |
| Journal of Neurotrauma | Resuscitation with pooled and pathogen-reduced plasma attenuates the increase in brain water content following traumatic brain injury and hemorrhagic shock in rats the rat. [doi/10.1089/neu.2016.4574] |
| British Journal of Anaesthesia | Volume kinetics of Ringer's lactate solution in acute inflammatory disease [10.1016:j.bja.2018.04.023] |
| Arteriosclerosis, Thrombosis, and?Vascular Biology | MPO (myeloperoxidase) reduces endothelial glycocalyx thickness dependent on its cationic charge [Pubmed:29903730] |
| Shock | Plasma resuscitation improved survival in a cecal ligation and puncture rat model of sepsis [Pubmed:28591008] |
| Shock | Experimental models of endotheliopathy: impact of shock severity [Pubmed:28697004] |
| Journal of?Trauma?and?Acute?Care?Surgery | Effects of propranolol and clonidine on brain edema, blood-brain barrier permeability, and endothelial glycocalyx disruption after fluid percussion brain injury in the rat [Pubmed:28930945] |
| The Endothelial Glycocalyx in the Peritoneal Microcirculation of Rats with Chronic Kidney Failure Exposed to Dialysis Solutions [173015_06.pdf] | |
| Journal of Surgical Research | Plasma ameliorates endothelial dysfunction in burn injury [Doi: 10.1016/j.jss.2018.08.027] |
| Journal of Anesthesia | Dexmedetomidine preserves the endothelial glycocalyx and improves survival in a rat heatstroke model [Pubmed: 30374889] |
| Anaesthesia | Postoperative microcirculatory perfusion and endothelial glycocalyx shedding following cardiac surgery with cardiopulmonary bypass [Pubmed: 30687934] |
| Journal of Cardiothoracic and Vascular Anesthesial | Microvascular alterations during cardiac surgery using a heparin or phosphorylcholine coated circuit [Pubmed: 31787433] |
| Hepatobiliary & Pancreatic Diseases International | Increased syndecan-1 and glypican-3 predict poor perinatal outcome and treatment resistance in intrahepatic cholestasis: Syndecan-1, glypican-3 and ICP [Pubmed: 31919038] |
| Heparin Binding Protein and Endothelial Glycocalyx Markers in Severe COVID-19–A Prospective Observational Cohort Study [] | |
| INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES | Newly Developed Recombinant Antithrombin Protects the Endothelial Glycocalyx in an Endotoxin-Induced Rat Model of Sepsis [33375342] |
| Microvasc Res | Effect of liraglutide on microcirculation in rat model with absolute insulin deficiency [34119534] |
| J Gerontol A Biol Sci Med Sci | The effect of pre-operative methylprednisolone on postoperative delirium in elderly patients undergoing gastrointestinal surgery: a randomized, double-blind, placebo?… [34423832] |
| Shock | Inhalation of 2% hydrogen improves survival rate and attenuates shedding of vascular endothelial glycocalyx in rats with heat stroke [34524269] |
| Exp Anim | Dexmedetomidine suppresses serum syndecan-1 elevation and improves survival in a rat hemorrhagic shock model [Pubmed:35110424] |
| Journal of Emergency Medicine | Resuscitation of hemorrhagic shock using normal saline does not damage the glycocalyx in the immediate resuscitation phase [] |
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