組織金屬蛋白酶抑制因子3(TIMP3)檢測試劑盒DIY材料(酶聯免疫吸附試驗法)
ELISA Kit DIY Materials for Tissue Inhibitors Of Metalloproteinase 3 (TIMP3)
SFD; TIMP Metallopeptidase Inhibitor 3; Metallopeptidase Inhibitor 3; Sorsby Fundus Dystrophy; Pseudoinflammatory
- 編號KSA129Ra01
- 物種Rattus norvegicus (Rat，大鼠) 相同的名稱，不同的物種。
- 試劑內容 包被抗體、檢測抗體、標準品、HRP標記的鏈霉親和素、TMB底物、96孔板
- 可檢測樣本Serum, plasma, tissue homogenates and other biological fluids.
- 可適用原理雙抗夾心酶聯免疫吸附試驗法測抗原
- 可檢測范圍0.156-10ng/mL
- 可適靈敏度0.068ng/mL
- 應用自己動手制作ELISA試劑盒的主要材料
- 下載 英文說明書 中文說明書
- 規格 96T*596T*10 96T*20 96T*50 96T*100
- 價格 ¥ 7387 ¥ 12312 ¥ 22162 ¥ 43092 ¥ 73872
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產品包裝(模擬)
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產品包裝(模擬)
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通過ISO 9001、ISO 13485質量體系認證
特異性
試劑盒中的抗體對組織金屬蛋白酶抑制因子3(TIMP3)具有高靈敏度和高特異性，經檢測與其它相似物質無明顯交叉反應。
用法
1.用100μL每孔的工作濃度的包被抗體包被96孔板，4度孵育過夜或者37度孵育2小時；
2.洗板1次；
3.用200μL每孔的工作濃度的封閉液,37度孵育1.5小時；
4.洗板1次。酶標板即可用于ELISA實驗，后續使用方法與ELISA同。
儲存
抗體，標準品和HRP標記的鏈霉親和素在-20度保存，TMB需在4度保存，96孔板在室溫保存。所有組分保質期為1年，試劑一旦開封，在4度保存時間為1個月。
配套試劑
增值服務
相關產品
| 編號 | 適用物種：Rattus norvegicus (Rat，大鼠) | 應用(僅供研究使用，不用于臨床診斷！) |
| APA129Ra01 | 組織金屬蛋白酶抑制因子3(TIMP3)活性蛋白 | Cell?culture;?Activity?Assays. |
| RPA129Ra01 | 組織金屬蛋白酶抑制因子3(TIMP3)重組蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| PAA129Ra01 | 組織金屬蛋白酶抑制因子3(TIMP3)多克隆抗體 | WB; IHC; ICC; IP. |
| MAA129Ra21 | 組織金屬蛋白酶抑制因子3(TIMP3)單克隆抗體 | WB; IHC; ICC; IP. |
| SEA129Ra | 組織金屬蛋白酶抑制因子3(TIMP3)檢測試劑盒(酶聯免疫吸附試驗法) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| SCA129Ra | 組織金屬蛋白酶抑制因子3(TIMP3)檢測試劑盒(化學發光免疫分析法) | Chemiluminescent immunoassay for Antigen Detection. |
| LMA129Ra | 組織金屬蛋白酶抑制因子3(TIMP3)等多因子檢測試劑盒(流式熒光發光法) | FLIA Kit for Antigen Detection. |
| KSA129Ra01 | 組織金屬蛋白酶抑制因子3(TIMP3)檢測試劑盒DIY材料(酶聯免疫吸附試驗法) | 自己動手制作ELISA試劑盒的主要材料 |
參考文獻
| 雜志 | 參考文獻 |
| Neuroimmunomodulation | Upregulated Expression of Matrix Metalloproteinases and Tissue Inhibitors of Matrix Metalloproteinases in BALB/c Mouse Brain Challenged with Japanese Encephalitis Virus [Pubmed: source] |
| Experimental Biology and Medicine | Acidic pH conditions mimicking degenerative intervertebral discs impair the survival and biological behavior of human adipose-derived mesenchymal stem cells [Rsmjournals: Source] |
| PLoS ONE | A Combinatorial Relative Mass Value Evaluation of Endogenous Bioactive Proteins in Three-Dimensional Cultured Nucleus Pulposus Cells of Herniated Intervertebral Discs: Identification of Potential Target Proteins for Gene Therapeutic Approaches [Plosone: Source] |
| Physiol. Res. | The unwounded skin remodeling in animal models of diabetes types 1 and 2. [Pubmed: 24020818] |
| VirusDisease | Circulating levels of matrix metalloproteinases and tissue inhibitors of matrix metalloproteinases during Japanese encephalitis virus infection [Pubmed:26925446] |
| Acta Pharmacol Sin. | Pharmacological evidence: a new therapeutic approach to the treatment of chronic heart failure through SUR2B/Kir6.1 channel in endothelial cells. [pubmed:27890915] |
| Biochemical genetics | Comparison of Selected Protein Levels in Tumour and Surgical Margin in a Group of Patients with Oral Cavity Cancer [pubmed:28421310] |
| International Journal of Cancer | Circulating and tissue IMP3 levels are correlated with poor survival in renal cell carcinoma [Pubmed: 30650187] |
| J. Pers. Med | Altered TIMP-3 Levels in the Cerebrospinal Fluid and Plasma of Patients with Alzheimer's Disease [Pubmed:35629249] |
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