心肌肌鈣蛋白T(TNNT2)檢測試劑盒DIY材料(酶聯免疫吸附試驗法)
ELISA Kit DIY Materials for Troponin T Type 2, Cardiac (TNNT2)
CMH2; cTnT; TnTc; Cardiomyopathy,Hypertrophic 2; Cardiac muscle troponin T
- 編號KSD232Hu01
- 物種Homo sapiens (Human，人) 相同的名稱，不同的物種。
- 試劑內容 包被抗體、檢測抗體、標準品、HRP標記的鏈霉親和素、TMB底物、96孔板
- 可檢測樣本Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids.
- 可適用原理雙抗夾心酶聯免疫吸附試驗法測抗原
- 可檢測范圍15.6-1000pg/mL
- 可適靈敏度6.4pg/mL
- 應用自己動手制作ELISA試劑盒的主要材料
- 下載 英文說明書 中文說明書
- 規格 96T*596T*10 96T*20 96T*50 96T*100
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產品包裝(模擬)
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產品包裝(模擬)
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通過ISO 9001、ISO 13485質量體系認證
特異性
試劑盒中的抗體對心肌肌鈣蛋白T(TNNT2)具有高靈敏度和高特異性，經檢測與其它相似物質無明顯交叉反應。
用法
1.用100μL每孔的工作濃度的包被抗體包被96孔板，4度孵育過夜或者37度孵育2小時；
2.洗板1次；
3.用200μL每孔的工作濃度的封閉液,37度孵育1.5小時；
4.洗板1次。酶標板即可用于ELISA實驗，后續使用方法與ELISA同。
儲存
抗體，標準品和HRP標記的鏈霉親和素在-20度保存，TMB需在4度保存，96孔板在室溫保存。所有組分保質期為1年，試劑一旦開封，在4度保存時間為1個月。
配套試劑
增值服務
相關產品
| 編號 | 適用物種：Homo sapiens (Human，人) | 應用(僅供研究使用，不用于臨床診斷！) |
| RPD232Hu01 | 心肌肌鈣蛋白T(TNNT2)重組蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| RPD232Hu03 | 心肌肌鈣蛋白T(TNNT2)重組蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| RPD232Hu04 | 心肌肌鈣蛋白T(TNNT2)重組蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| RPD232Hu02 | 心肌肌鈣蛋白T(TNNT2)重組蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| APD232Hu01 | 心肌肌鈣蛋白T(TNNT2)活性蛋白 | Cell?culture;?Activity?Assays. |
| PAD232Hu03 | 心肌肌鈣蛋白T(TNNT2)多克隆抗體 | WB; IHC; ICC; IP. |
| PAD232Hu01 | 心肌肌鈣蛋白T(TNNT2)多克隆抗體 | WB; IHC; ICC; IP. |
| PAD232Hu02 | 心肌肌鈣蛋白T(TNNT2)多克隆抗體 | WB; IHC; ICC; IP. |
| LAD232Hu81 | 心肌肌鈣蛋白T(TNNT2)多克隆抗體(異硫氰酸熒光素標記) | WB; IHC; ICC; IF. |
| LAD232Hu71 | 心肌肌鈣蛋白T(TNNT2)多克隆抗體(生物素標記) | WB; IHC; ICC. |
| MAD232Hu23 | 心肌肌鈣蛋白T(TNNT2)單克隆抗體 | WB; IHC; ICC; IP. |
| MAD232Hu29 | 心肌肌鈣蛋白T(TNNT2)單克隆抗體 | WB; IHC; ICC; IP. |
| MAD232Hu24 | 心肌肌鈣蛋白T(TNNT2)單克隆抗體 | WB; IHC; ICC; IP. |
| MAD232Hu25 | 心肌肌鈣蛋白T(TNNT2)單克隆抗體 | WB; IHC; ICC; IP. |
| MAD232Hu21 | 心肌肌鈣蛋白T(TNNT2)單克隆抗體 | WB; IHC; ICC; IP. |
| MAD232Hu22 | 心肌肌鈣蛋白T(TNNT2)單克隆抗體 | WB; IHC; ICC; IP. |
| MAD232Hu27 | 心肌肌鈣蛋白T(TNNT2)單克隆抗體 | WB; IHC; ICC; IP. |
| MAD232Hu28 | 心肌肌鈣蛋白T(TNNT2)單克隆抗體 | WB; IHC; ICC; IP. |
| FAD232Hu08 | 抗心肌肌鈣蛋白T(TNNT2)單克隆抗體 | FCM |
| FAD232Hu02 | 抗心肌肌鈣蛋白T(TNNT2)單克隆抗體 | Flow cytometry. |
| FAD232Hu04 | 抗心肌肌鈣蛋白T(TNNT2)單克隆抗體 | Flow cytometry. |
| FAD232Hu06 | 抗心肌肌鈣蛋白T(TNNT2)單克隆抗體 | Flow cytometry. |
| SED232Hu | 心肌肌鈣蛋白T(TNNT2)檢測試劑盒(酶聯免疫吸附試驗法) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| HED232Hu | 心肌肌鈣蛋白T(TNNT2)檢測試劑盒(酶聯免疫吸附試驗法,高敏型) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| MED232Hu | 心肌肌鈣蛋白T(TNNT2)檢測試劑盒(酶聯免疫吸附試驗法,小樣本) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| SCD232Hu | 心肌肌鈣蛋白T(TNNT2)檢測試劑盒(化學發光免疫分析法) | Chemiluminescent immunoassay for Antigen Detection. |
| LMD232Hu | 心肌肌鈣蛋白T(TNNT2)等多因子檢測試劑盒(流式熒光發光法) | FLIA Kit for Antigen Detection. |
| KSD232Hu01 | 心肌肌鈣蛋白T(TNNT2)檢測試劑盒DIY材料(酶聯免疫吸附試驗法) | 自己動手制作ELISA試劑盒的主要材料 |
參考文獻
| 雜志 | 參考文獻 |
| Basic & Clinical Pharmacology & Toxicology | Cardioprotective Effect of Sulphonated Formononetin on Acute Myocardial Infarction in Rats [PubMed: 21232020] |
| Basic & Clinical Pharmacology & Toxicology | Cardioprotective Effect of SMND-309, A Novel Derivate of Salvianolic Acid B on Acute Myocardial Infarction in Rats [Wiley: source] |
| European Journal of Pharmacology | Protective roles of Asperosaponin VI, a triterpene saponin isolated from Dipsacus asper Wall on acute myocardial infarction in rats [ScienceDirect: S0014299909009984] |
| Journal of translational medicine | The altered expression profile of microRNAs in cardiopulmonary bypass canine models and the effects of mir-499 on myocardial ischemic reperfusion injury [PubMed: PMC3694448] |
| Oral Diseases | Salivary high‐sensitivity cardiac troponin T levels in patients with acute myocardial infarction [Pubmed: 22834943] |
| An International Journal of Pharmaceutical Sciences | In vivo and in vitro cardioprotective effects of Panax quinquefolium 20 (S)-protopanaxadiol saponins (PQDS), isolated from Panax quinquefolium [Pubmed: 23700796] |
| Journal of Animal and Feed Sciences, | The effects of caloric restriction and age on thyroid hormone signalling in the heart of rats [Pl:Source] |
| Liver Int. | Cardiomyopathy reverses with recovery of liver injury, cholestasis and cholanemia in mouse model of biliary fibrosis [Pubmed:24330504] |
| Cardiovasc Toxicol. | Cardioprotective Effect of Rhizomes of Acorus gramineus Against Isoproterenol-Induced Cardiac Damage in Pigs [Pubmed:24420420] |
| British Journal of Pharmacology | Glutathione administration reduces mitochondrial damage and shifts cell death from necrosis to apoptosis in ageing diabetic mice hearts during exercise [Pubmed:25039894] |
| Chemico-Biological Interactions | Crocin protects against doxorubicin-induced myocardial toxicity in rats through down-reCavia (Guinea pig )lation of inflammatory and apoptic pathways [Pubmed:26807765] |
| The Journal of Pharmacology and Experimental Therapeuties | The Cardenolide Glycoside Acovenoside A Affords Protective Activity in Doxorubicin-Induced Cardiotoxicity in Mice [Pubmed:27247000] |
| The Journal of Maternal-Fetal & Neonatal Medicine | Troponins, heat shock proteins and glycogen phosphorylase BB in umbilical cord blood of complicated pregnancies. [pubmed:27937003] |
| PLoS One. | Outer Balloon Ligation Increases Success Rate of Ischemia-Reperfusion Injury Model in Mice. [pubmed:27907155] |
| Biomedicine & Pharmacotherapy | Rosuvastatin improves myocardial and neurological outcomes after asphyxial cardiac arrest and cardiopulmonary resuscitation in rats [pubmed:28076830] |
| PLoS One | Imatinib attenuates cardiac fibrosis by inhibiting platelet-derived growth factor receptors activation in isoproterenol induced model [pubmed:28570599] |
| EXPERIMENTAL AND THERAPEUTIC MEDICINE | Cardioprotective effect of Notch signaling on the development of myocardial infarction complicated by diabetes mellitus [pubmed:29042932] |
| PLoS One | Increases in plasma corin levels following experimental myocardial infarction reflect the severity of ischemic injury [Pubmed: 30192780] |
| PLoS One | Tissue suction-mediated gene transfer to the beating heart in mice [Pubmed: 32027678] |
| Journal of Human Kinetics | Hormonal Response to Incremental and Continuous Exercise in Cyclists with Left Ventricle Hypertrophy [Pubmed: 32148580] |
| BIOMEDICINE & PHARMACOTHERAPY | Chlorogenic acid prevents acute myocardial infarction in rats by reducing inflammatory damage and oxidative stress [Pubmed: 33022535] |
| Toxics | Norepinephrine Leads to More Cardiopulmonary Toxicities than Epinephrine by Catecholamine Overdose in Rats [Pubmed: 32947820] |
| Elife | Iron derived from autophagy-mediated ferritin degradation induces cardiomyocyte death and heart failure in mice [33526170] |
| Cell Metabolism | Pharmacological inhibition of arachidonate 12-lipoxygenase ameliorates myocardial ischemia-reperfusion injury in multiple species [34536344] |
| Anal Bioanal Chem | Development of a novel ssDNA aptamer targeting cardiac troponin I and its clinical applications [34673993] |
| Experimental and Therapeutic Medicine | Hydrogen therapy after resuscitation improves myocardial injury involving inhibition of autophagy in an asphyxial rat model of cardiac arrest [Pubmed:35495584] |
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