胰蛋白酶原激活肽(TAP)檢測試劑盒DIY材料(酶聯免疫吸附試驗法)
ELISA Kit DIY Materials for Trypsinogen Activation Peptide (TAP)
- 編號KSA634Ra11
- 物種Rattus norvegicus (Rat,大鼠) 相同的名稱,不同的物種。
- 試劑內容 包被抗體、生物素標記的競爭物、標準品、HRP標記的鏈霉親和素、TMB底物、96孔板
- 可檢測樣本Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids.
- 可適用原理競爭抑制酶聯免疫吸附試驗法測抗原
- 可檢測范圍123.46-10000pg/mL
- 可適靈敏度48.6pg/mL
- 應用Main materials for "Do It (ELISA Kit) Yourself".
- 下載 英文說明書 中文說明書
- 規格 96T*596T*10 96T*20 96T*50 96T*100
- 價格 ¥ 7387 ¥ 12312 ¥ 22162 ¥ 43092 ¥ 73872
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特異性
試劑盒中的抗體對胰蛋白酶原激活肽(TAP)具有高靈敏度和高特異性,經檢測與其它相似物質無明顯交叉反應。
用法
1.用100μL每孔的工作濃度的包被抗體包被96孔板,4度孵育過夜或者37度孵育2小時;
2.洗板1次;
3.用200μL每孔的工作濃度的封閉液,37度孵育1.5小時;
4.洗板1次。酶標板即可用于ELISA實驗,后續使用方法與ELISA同。
儲存
抗體,標準品和HRP標記的鏈霉親和素在-20度保存,TMB需在4度保存,96孔板在室溫保存。所有組分保質期為1年,試劑一旦開封,在4度保存時間為1個月。
配套試劑
增值服務
相關產品
編號 | 適用物種:Rattus norvegicus (Rat,大鼠) | 應用(僅供研究使用,不用于臨床診斷!) |
CPA634Ra11 | 胰蛋白酶原激活肽(TAP)牛血清白蛋白偶聯物 | Immunogen; SDS-PAGE; WB. |
CPA634Ra21 | 胰蛋白酶原激活肽(TAP)卵白蛋白偶聯物 | Immunogen; SDS-PAGE; WB. |
CEA634Ra | 胰蛋白酶原激活肽(TAP)檢測試劑盒(酶聯免疫吸附試驗法) | Enzyme-linked immunosorbent assay for Antigen Detection. |
LMA634Ra | 胰蛋白酶原激活肽(TAP)等多因子檢測試劑盒(流式熒光發光法) | FLIA Kit for Antigen Detection. |
KSA634Ra11 | 胰蛋白酶原激活肽(TAP)檢測試劑盒DIY材料(酶聯免疫吸附試驗法) | Main materials for "Do It (ELISA Kit) Yourself". |
參考文獻
雜志 | 參考文獻 |
Diabetologia | Diabetes aggravates acute pancreatitis and inhibits pancreas regeneration in mice [SpringerLink: x4l01435t34452jr] |
Molecular Biology Reports | Prior peritoneal lavage with hot 0.9% saline induces HSP70 expression and protects against cerulein-induced acute pancreatitis in rats [Pubmed: 23096089] |
Journal of the Pancreas | Case report of mannose-binding lectin (MBL) deficiency and postoperative sepsis and coagulopathy in a patient following total pancreatectomy for chronic pancreatitis. [Pubmed:25262717] |
Journal of Gastroenterology | A small molecule inhibitor of NFκB blocks ER stress and the NLRP3 inflammasome and prevents progression of pancreatitis [pubmed:27418337] |
Indian Journal of Pharmaceutical Sciences | Effects of Calcium Channel Blockers on Trypsinogen Activation and Severity of Cerulein-induced Acute Pancreatitis in Rats [IJPS:Source] |
Tropical Journal of Pharmaceutical Research | Investigation of non-pharmaceutical cures for acute pancreatitis induced by cerulein in rats [] |
Pancreas | The Regulatory Effect of the Kinase Inhibitor PD98059 on Autophagic Flux During Trypsinogen Activation in Pancreatic Acinar Cells [Pubmed: 32011537] |
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