玻連蛋白(VTN)檢測試劑盒DIY材料(酶聯免疫吸附試驗法)
ELISA Kit DIY Materials for Vitronectin (VTN)
V75; VN; VNT; Serum Spreading Factor; Somatomedin B; Complement S-Protein
- 編號KSB041Ra01
- 物種Rattus norvegicus (Rat,大鼠) 相同的名稱,不同的物種。
- 試劑內容 包被抗體、檢測抗體、標準品、HRP標記的鏈霉親和素、TMB底物、96孔板
- 可檢測樣本Plasma.
- 可適用原理雙抗夾心酶聯免疫吸附試驗法測抗原
- 可檢測范圍7.81-500ng/mL
- 可適靈敏度3.7ng/mL
- 應用Main materials for "Do It (ELISA Kit) Yourself".
- 下載 英文說明書 中文說明書
- 規格 96T*596T*10 96T*20 96T*50 96T*100
- 價格 ¥ 7798 ¥ 12996 ¥ 23393 ¥ 45486 ¥ 77976
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特異性
試劑盒中的抗體對玻連蛋白(VTN)具有高靈敏度和高特異性,經檢測與其它相似物質無明顯交叉反應。
用法
1.用100μL每孔的工作濃度的包被抗體包被96孔板,4度孵育過夜或者37度孵育2小時;
2.洗板1次;
3.用200μL每孔的工作濃度的封閉液,37度孵育1.5小時;
4.洗板1次。酶標板即可用于ELISA實驗,后續使用方法與ELISA同。
儲存
抗體,標準品和HRP標記的鏈霉親和素在-20度保存,TMB需在4度保存,96孔板在室溫保存。所有組分保質期為1年,試劑一旦開封,在4度保存時間為1個月。
配套試劑
增值服務
相關產品
編號 | 適用物種:Rattus norvegicus (Rat,大鼠) | 應用(僅供研究使用,不用于臨床診斷!) |
RPB041Ra01 | 玻連蛋白(VTN)重組蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
APB041Ra01 | 玻連蛋白(VTN)活性蛋白 | Cell?culture;?Activity?Assays. |
PAB041Ra01 | 玻連蛋白(VTN)多克隆抗體 | WB; IHC; ICC; IP. |
MAB041Ra21 | 玻連蛋白(VTN)單克隆抗體 | WB; IHC; ICC; IP. |
SEB041Ra | 玻連蛋白(VTN)檢測試劑盒(酶聯免疫吸附試驗法) | Enzyme-linked immunosorbent assay for Antigen Detection. |
LMB041Ra | 玻連蛋白(VTN)等多因子檢測試劑盒(流式熒光發光法) | FLIA Kit for Antigen Detection. |
KSB041Ra01 | 玻連蛋白(VTN)檢測試劑盒DIY材料(酶聯免疫吸附試驗法) | Main materials for "Do It (ELISA Kit) Yourself". |
參考文獻
雜志 | 參考文獻 |
Biomedical Engineering and Informatics | Application of iTRAQ quantitative proteomics in identification of serum biomarkers in breast cancer [IEEE: 6098563] |
PLoS ONE | Identification of Altered Plasma Proteins by Proteomic Study in Valvular Heart Diseases and the Potential Clinical Significance [PubMed: PMC3754973] |
Liver International | Identification of candidate biomarkers for hepatocellular carcinoma in plasma of HCV-infected cirrhotic patients by 2-D DIGE [Pubmed: 23944848] |
Analytical Biochemistry | A multianalytical approach to investigate the effect of nanofiltration on plasma-derived factor IX clinical lots [Pubmed:29154788] |
Journal of Materials Chemistry B | Serum protein adsorption profile on BCP ceramic and influence of the elevated adsorption of adhesive proteins on the behaviour of MSCs [Doi: 10.1039/C8TB02283F?] |
JOURNAL OF NEPHROLOGY | Urinary vitronectin identifies patients with high levels of fibrosis in kidney grafts [33275196] |
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