1,25-二羥基維生素D3(DHVD3)檢測試劑盒(酶聯免疫吸附試驗法)
ELISA Kit for 1,25-Dihydroxyvitamin D3 (DHVD3)
DH-VD3; Calcitriol; Rocaltrol; Calcijex; 1,25-(OH)2D3; 1,25-Dihydroxycholecalciferol; 1,25-Dihydroxy Vitamin D3
- 編號CEA467Ge
- 物種Pan-species (General，通用) 相同的名稱，不同的物種。
- 實驗方法競爭抑制
- 反應時長2h
- 檢測范圍24.69-2,000pg/mL
- 靈敏度最小可檢測劑量小于等于9.92pg/mL.
- 樣本類型Serum, plasma and other biological fluids
- 下載 英文說明書 中文說明書
- 規格 48T96T 96T*5 96T*10 96T*100
- 價格 ¥ 3342 ¥ 4774 ¥ 21481 ¥ 40576 ¥ 334152
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產品包裝(模擬)
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產品包裝(模擬)
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實驗結果圖
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標準曲線圖
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通過ISO 9001、ISO 13485質量體系認證
特異性
本試劑盒用于檢測1,25-二羥基維生素D3(DHVD3)，經檢測與其它相似物質無明顯交叉反應。
由于受到技術及樣本來源的限制，不可能完成對所有相關或相似物質交叉反應檢測，因此本試劑盒有可能與未經檢測的其它物質有交叉反應。
回收率
分別于定值血清及血漿樣本中加入一定量的1,25-二羥基維生素D3(DHVD3)（加標樣品），重復測定并計算其均值，回收率為測定值與理論值的比率。
| 樣本 | 回收率范圍(%) | 平均回收率(%) |
| serum(n=5) | 95-104 | 101 |
| EDTA plasma(n=5) | 83-95 | 87 |
| heparin plasma(n=5) | 86-98 | 95 |
精密度
精密度用樣品測定值的變異系數CV表示。CV(%) = SD/mean×100
批內差：取同批次試劑盒對低、中、高值定值樣本進行定量檢測,每份樣本連續測定20 次，分別計算不同濃度樣本的平均值及SD值。
批間差：選取3個不同批次的試劑盒分別對低、中、高值定值樣本進行定量測定，每個樣本使用同一試劑盒重復測定8次，分別計算不同濃度樣本的平均值及SD值。
批內差： CV<10%
批間差： CV<12%
線性
在定值血清及血漿樣本內加入適量的1,25-二羥基維生素D3(DHVD3)，并倍比稀釋成1:2，1:4，1:8，1:16的待測樣本，線性范圍即為稀釋后樣本中1,25-二羥基維生素D3(DHVD3)含量的測定值與理論值的比率。
| 樣本 | 1:2 | 1:4 | 1:8 | 1:16 |
| serum(n=5) | 93-101% | 81-92% | 88-98% | 89-96% |
| EDTA plasma(n=5) | 87-102% | 92-101% | 92-99% | 92-101% |
| heparin plasma(n=5) | 78-89% | 93-101% | 78-103% | 96-103% |
穩定性
經測定，試劑盒在有效期內按推薦溫度保存，其活性降低率小于5%。
為減小外部因素對試劑盒破壞前后檢測值的影響，實驗室的環境條件需盡量保持一致，尤其是實驗室內溫度、濕度及溫育條件。其次由同一實驗員來進行操作可減少人為誤差。
實驗流程
1. 實驗前標準品、試劑及樣本的準備；
2. 加樣（標準品及樣本）50µL，
加入50µL檢測液A(臨用前配制);
37°C溫育1小時。
3. 洗板3次；
4. 加檢測溶液B100µL，37°C孵育30分鐘；
5. 洗板5次；
6. 加TMB底物90µL，37°C孵育10-20分鐘；
7. 加終止液50µL，立即450nm讀數。
實驗原理
本試劑盒應用競爭抑制酶聯免疫分析法測定標本中待測物質水平。將DHVD3單克隆抗體包被微孔板，制成固相載 體，往包被抗體的微孔中同時加入生物素標記的DHVD3類似物和待測抗原(標準品或樣本)，待測抗原與生物素標記DHVD3類似物對特異性抗體進行競爭結合。溫育后經洗滌去掉未結合物，然后加入HRP標記的親和素，經過溫育和徹底洗滌后加入底物TMB顯色。TMB在過氧化物酶的催化下轉化成藍色，并在酸的作用下轉化成最終的黃色。待測標本濃度越高，標記抗原和抗體的結合就越受到抑制，顯色愈淺。顯色的深淺與酶量呈正相關，而與樣品中待測物質含量呈負相關。用酶標儀在450nm波長下測定吸光度（O.D.值），計算樣品濃度。
相關產品
| 編號 | 適用物種：Pan-species (General，通用) | 應用(僅供研究使用，不用于臨床診斷！) |
| CEA467Ge | 1,25-二羥基維生素D3(DHVD3)檢測試劑盒(酶聯免疫吸附試驗法) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| LMA467Ge | 1,25-二羥基維生素D3(DHVD3)等多因子檢測試劑盒(流式熒光發光法) | FLIA Kit for Antigen Detection. |
參考文獻
| 雜志 | 參考文獻 |
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| BMC Medicine | Calcium-deficiency assessment and biomarker identification by an integrated urinary metabonomics analysis [PubMed: PMC3652781] |
| Nephrol Dial Transplant. | Treatment of established left ventricular hypertrophy with fibroblast growth factor receptor blockade in an animal model of CKD [Pubmed:24875663] |
| Endocrinology. | Vitamin D Prevents Podocyte Injury via Regulation of Macrophage M1/M2 Phenotype in Diabetic Nephropathy Rats [Pubmed:25188527] |
| Tumor Biology | Calcitriol?induced?redox?imbalance and DNA breakage in cells sharing a common metabolic feature of malignancies: Interaction with cellular copper (II) ions leads to the production of reactive oxygen species. [Pubmed:25547436] |
| International Journal of Life Sciences Research | Impact of Curcumin and Flaxseed Oil on Bone Turnover in Glucocorticoid-Injected Female Rats [profile:303311214] |
| Protective Effect of Soybean and Mushroom against Glucocorticoid-Induced Osteoporosis in Female Rats [:] | |
| Apoptosis | Calcitriol–copper interaction leads to non enzymatic, reactive oxygen species mediated DNA breakage and modulation of cellular redox scavengers in hepatocellular … [article:10.1007] |
| ?in vivo | Effect of Calcitriol on FGF23 Level in Healthy Adults and its Dependence on Phosphate Level [pubmed:28064234] |
| Hypertension Research | Glucocorticoid exposure induces preeclampsia via dampening 1,25-dihydroxyvitamin D3 [pubmed:29093562] |
| International?Journal?of?Clinical?and?Experimental?Medicine | Combined effect of pulsed electromagnetic fields and narrowband ultraviolet B on bone metabolism in glucocorticoid-treated rats [] |
| Journal of Clinical and Translational Endocrinology | Dobesity: relationship between vitamin d deficiency, obesity and sclerostin as a novel biomarker of bone metabolism [] |
| International Journal of Environmental Research and Public Health | Alleviating Effect of α-Lipoic Acid and Magnesium on Cadmium-Induced Inflammatory Processes, Oxidative Stress and Bone Metabolism Disorders in Wistar Rats [Pubmed: 31739465] |
| Ecotoxicology and Environmental Safety | Sodium dehydroacetate induces cardiovascular toxicity associated with Ca2+ imbalance in zebrafish [] |
| EXPERIMENTAL AND MOLECULAR MEDICINE | Metabolic activities affect femur and lumbar vertebrae remodeling, and anti-resorptive risedronate disturbs femoral cortical bone remodeling [33436949] |
| Life Sci | SWIM domain protein ZSWIM4 is required for JAK2 inhibition resistance in breast cancer [34102191] |
| Nutrients | Effect of Dapagliflozin and Magnesium Supplementation on Renal Magnesium Handling and Magnesium Homeostasis in Metabolic Syndrome [34836340] |
