親環(huán)素A(CYPA)檢測試劑盒(酶聯(lián)免疫吸附試驗法)
ELISA Kit for Cyclophilin A (CYPA)
PPIA; CyP-A; CYPH; Peptidylprolyl Isomerase A; Peptidyl-Prolyl Isomerase A; Peptidyl-prolyl cis-trans isomerase A; Cyclosporin A-binding protein
- 編號SEA979Ra
- 物種Rattus norvegicus (Rat，大鼠) 相同的名稱，不同的物種。
- 實驗方法雙抗夾心
- 反應時長3h
- 檢測范圍1.25-80ng/mL
- 靈敏度最小可檢測劑量小于等于0.47ng/mL.
- 樣本類型serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
- 下載 英文說明書 中文說明書
- 規(guī)格 48T96T 96T*5 96T*10 96T*100
- 價格 ¥ 2873 ¥ 4104 ¥ 18468 ¥ 34884 ¥ 287280
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產(chǎn)品包裝(模擬)
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產(chǎn)品包裝(模擬)
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實驗結果圖
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標準曲線圖
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通過ISO 9001、ISO 13485質(zhì)量體系認證
特異性
本試劑盒用于檢測親環(huán)素A(CYPA)，經(jīng)檢測與其它相似物質(zhì)無明顯交叉反應。
由于受到技術及樣本來源的限制，不可能完成對所有相關或相似物質(zhì)交叉反應檢測，因此本試劑盒有可能與未經(jīng)檢測的其它物質(zhì)有交叉反應。
回收率
分別于定值血清及血漿樣本中加入一定量的親環(huán)素A(CYPA)（加標樣品），重復測定并計算其均值，回收率為測定值與理論值的比率。
| 樣本 | 回收率范圍(%) | 平均回收率(%) |
| serum(n=5) | 81-92 | 88 |
| EDTA plasma(n=5) | 92-101 | 98 |
| heparin plasma(n=5) | 92-99 | 96 |
精密度
精密度用樣品測定值的變異系數(shù)CV表示。CV(%) = SD/mean×100
批內(nèi)差：取同批次試劑盒對低、中、高值定值樣本進行定量檢測,每份樣本連續(xù)測定20 次，分別計算不同濃度樣本的平均值及SD值。
批間差：選取3個不同批次的試劑盒分別對低、中、高值定值樣本進行定量測定，每個樣本使用同一試劑盒重復測定8次，分別計算不同濃度樣本的平均值及SD值。
批內(nèi)差： CV<10%
批間差： CV<12%
線性
在定值血清及血漿樣本內(nèi)加入適量的親環(huán)素A(CYPA)，并倍比稀釋成1:2，1:4，1:8，1:16的待測樣本，線性范圍即為稀釋后樣本中親環(huán)素A(CYPA)含量的測定值與理論值的比率。
| 樣本 | 1:2 | 1:4 | 1:8 | 1:16 |
| serum(n=5) | 88-103% | 85-99% | 88-104% | 98-105% |
| EDTA plasma(n=5) | 80-101% | 91-105% | 92-101% | 96-105% |
| heparin plasma(n=5) | 89-104% | 89-103% | 97-105% | 78-91% |
穩(wěn)定性
經(jīng)測定，試劑盒在有效期內(nèi)按推薦溫度保存，其活性降低率小于5%。
為減小外部因素對試劑盒破壞前后檢測值的影響，實驗室的環(huán)境條件需盡量保持一致，尤其是實驗室內(nèi)溫度、濕度及溫育條件。其次由同一實驗員來進行操作可減少人為誤差。
實驗流程
1. 實驗前標準品、試劑及樣本的準備；
2. 加樣（標準品及樣本）100µL，37°C孵育1小時；
3. 吸棄，加檢測溶液A100µL，37°C孵育1小時；
4. 洗板3次；
5. 加檢測溶液B100µL，37°C孵育30分鐘；
6. 洗板5次；
7. 加TMB底物90µL，37°C孵育10-20分鐘；
8. 加終止液50µL，立即450nm讀數(shù)。
實驗原理
將親環(huán)素A(CYPA)抗體包被于96孔微孔板中，制成固相載體，向微孔中分別加入標準品或標本，其中的親環(huán)素A(CYPA)與連接于固相載體上的抗體結合，然后加入生物素化的親環(huán)素A(CYPA)抗體，將未結合的生物素化抗體洗凈后，加入HRP標記的親和素，再次徹底洗滌后加入TMB底物顯色。TMB在過氧化物酶的催化下轉(zhuǎn)化成藍色，并在酸的作用下轉(zhuǎn)化成最終的黃色。顏色的深淺和樣品中的親環(huán)素A(CYPA)呈正相關。用酶標儀在450nm波長下測定吸光度(O.D.值)，計算樣品濃度。
增值服務
相關產(chǎn)品
| 編號 | 適用物種：Rattus norvegicus (Rat，大鼠) | 應用(僅供研究使用，不用于臨床診斷！) |
| RPA979Ra01 | 親環(huán)素A(CYPA)重組蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| PAA979Ra01 | 親環(huán)素A(CYPA)多克隆抗體 | WB; IHC; ICC; IP. |
| LAA979Ra71 | 親環(huán)素A(CYPA)多克隆抗體(生物素標記) | WB; IHC; ICC. |
| LAA979Ra81 | 親環(huán)素A(CYPA)多克隆抗體(異硫氰酸熒光素標記) | WB; IHC; ICC; IF. |
| MAA979Ra29 | 親環(huán)素A(CYPA)單克隆抗體 | WB; IHC; ICC; IP. |
| MAA979Ra24 | 親環(huán)素A(CYPA)單克隆抗體 | WB; IHC; ICC; IP. |
| MAA979Ra23 | 親環(huán)素A(CYPA)單克隆抗體 | WB; IHC; ICC; IP. |
| MAA979Ra22 | 親環(huán)素A(CYPA)單克隆抗體 | WB; IHC; ICC; IP. |
| MAA979Ra25 | 親環(huán)素A(CYPA)單克隆抗體 | WB; IHC; ICC; IP. |
| MAA979Ra21 | 親環(huán)素A(CYPA)單克隆抗體 | WB; IHC; ICC; IP. |
| MAA979Ra27 | 親環(huán)素A(CYPA)單克隆抗體 | WB; IHC; ICC; IP. |
| SEA979Ra | 親環(huán)素A(CYPA)檢測試劑盒(酶聯(lián)免疫吸附試驗法) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| LMA979Ra | 親環(huán)素A(CYPA)等多因子檢測試劑盒(流式熒光發(fā)光法) | FLIA Kit for Antigen Detection. |
| PSA979Ra01 | 親環(huán)素A(CYPA)抗體對 | ELISA; CLIA; ELISPOT; Luminex; Immunochromatography and other Immunoassays. |
| KSA979Ra01 | 親環(huán)素A(CYPA)檢測試劑盒DIY材料(酶聯(lián)免疫吸附試驗法) | Main materials for "Do It (ELISA Kit) Yourself". |
參考文獻
| 雜志 | 參考文獻 |
| Atherosclerosis | EMMPRIN and its ligand cyclophilin A regulate MT1-MMP, MMP-9 and M-CSF during foam cell formation [PubMed: 19758589] |
| Biomarkers | Cyclophilin-A: a novel biomarker for untreated male essential hypertension [Pubmed: 24164594] |
| Cardiovascular Diabetology | Plasma level of cyclophilin A is increased in patients with type 2 diabetes mellitus and suggests presence of vascular disease [Pubmed:24502618] |
| International Journal of Biological Sciences | Decrease in Plasma Cyclophilin A Concentration at 1 Month after Myocardial Infarction Predicts Better Left Ventricular Performance and Synchronicity at 6 Months: A Pilot Study in Patients with ST Elevation Myocardial Infarction [Pubmed:25552928] |
| Medicine | Cyclophilin A in Ruptured Intracranial Aneurysm [PubMed: 26426668] |
| Mol Cell Biochem | PPIA rs6850: A> G single-nucleotide polymorphism is associated with raised plasma cyclophilin A levels in patients with coronary artery disease [PubMed: 26702934] |
| Medicine (Baltimore) | Urinary Cyclophilin A as a New Marker for Diabetic Nephropathy: A Cross-Sectional Analysis of Diabetes Mellitus [PubMed: 26496315] |
| International Journal of Cardiology | Cyclophilin?A?modulates?bone?marrow-derived?CD117(+)?cells?and?enhances?ischemia-induced?angiogenesisvia?the?SDF-1/CXCR4?axis. [Pubmed:27057951] |
| Clinica?Chimica?Acta | Novel findings of secreted cyclophilin A in diabetic nephropathy and its association with renal protection of dipeptidyl peptidase 4 inhibitor [pubmed:27823952] |
| Cardiovascular?Diabetology | Cyclophilin?A?enhances?macrophage?differentiation?and?lipid?uptake?in?high?glucose?conditions: a cellular mechanism for accelerated macro vascular disease in diabetes mellitus. [pubmed:27809851] |
| Clinical?Science | Metformin attenuates effects of cyclophilin A on macrophages, reduces lipid uptake and secretion of cytokines by repressing decreased AMPK activity [Pubmed:29382697] |
| Journal of Molecular Histology | Cyclophilin a increases CD68 cell infiltration in rat experimental periodontitis [Pubmed:29318411] |
| Free?Radical?Research | Cyclophilin A and CD147 associate with progression of diabetic nephropathy [Pubmed: 30572748] |
| The Kaohsiung Journal of Medical Sciences | Association of cyclophilin A level and pulse pressure in predicting recurrence of cerebral infarction [Pubmed: 31670477] |
| Cells. | Cyclophilin A/EMMPRIN Axis Is Involved in Pro-Fibrotic Processes Associated with Thoracic Aortic Aneurysm of Marfan Syndrome Patients [Pubmed: 31936351] |
| JAMA Neurology | APPENDIX A: Relationship between cyclophilin A levels and matrix metalloproteinase-9 activity in cerebrospinal fluid of cognitively normal apolipoprotein E4 carriers?… [Pubmed: 24030206] |
| Menoufia Medical Journal | Urinary podocalyxin and cyclophilin A: markers for early detection of type 2 diabetic nephropathy [] |
| Toxins | Evaluation of the Therapeutic Effects of Protocatechuic Aldehyde in Diabetic Nephropathy [34437430] |
| Cell Death & Disease | Proteomic analysis reveals USP7 as a novel regulator of palmitic acid-induced hepatocellular carcinoma cell death [Pubmed:35732625] |
