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沉默調節(jié)蛋白1(SIRT1)檢測試劑盒(酶聯(lián)免疫吸附試驗法)

ELISA Kit for Sirtuin 1 (SIRT1)

SIR2L1; Silent Mating Type Information Regulation 2 Homolog 1; NAD-Dependent Deacetylase Sirtuin-1; SIR2-like protein 1; SirtT1 75 kDa fragment

  • 沉默調節(jié)蛋白1(SIRT1)檢測試劑盒(酶聯(lián)免疫吸附試驗法) 產品包裝(模擬)
  • 沉默調節(jié)蛋白1(SIRT1)檢測試劑盒(酶聯(lián)免疫吸附試驗法) 產品包裝(模擬)
  • 沉默調節(jié)蛋白1(SIRT1)檢測試劑盒(酶聯(lián)免疫吸附試驗法) 實驗結果圖
  • SEE912Mu.jpg 標準曲線圖
  • Certificate 通過ISO 9001、ISO 13485質量體系認證

特異性

本試劑盒用于檢測沉默調節(jié)蛋白1(SIRT1),經檢測與其它相似物質無明顯交叉反應。
由于受到技術及樣本來源的限制,不可能完成對所有相關或相似物質交叉反應檢測,因此本試劑盒有可能與未經檢測的其它物質有交叉反應。

精密度

精密度用樣品測定值的變異系數(shù)CV表示。CV(%) = SD/mean×100
批內差:取同批次試劑盒對低、中、高值定值樣本進行定量檢測,每份樣本連續(xù)測定20 次,分別計算不同濃度樣本的平均值及SD值。
批間差:選取3個不同批次的試劑盒分別對低、中、高值定值樣本進行定量測定,每個樣本使用同一試劑盒重復測定8次,分別計算不同濃度樣本的平均值及SD值。
批內差: CV<10%
批間差: CV<12%

穩(wěn)定性

經測定,試劑盒在有效期內按推薦溫度保存,其活性降低率小于5%。
為減小外部因素對試劑盒破壞前后檢測值的影響,實驗室的環(huán)境條件需盡量保持一致,尤其是實驗室內溫度、濕度及溫育條件。其次由同一實驗員來進行操作可減少人為誤差。

實驗流程

1. 實驗前標準品、試劑及樣本的準備;
2. 加樣(標準品及樣本)100µL,37°C孵育1小時;
3. 吸棄,加檢測溶液A100µL,37°C孵育1小時;
4. 洗板3次;
5. 加檢測溶液B100µL,37°C孵育30分鐘;
6. 洗板5次;
7. 加TMB底物90µL,37°C孵育10-20分鐘;
8. 加終止液50µL,立即450nm讀數(shù)。

實驗原理

將沉默調節(jié)蛋白1(SIRT1)抗體包被于96孔微孔板中,制成固相載體,向微孔中分別加入標準品或標本,其中的沉默調節(jié)蛋白1(SIRT1)與連接于固相載體上的抗體結合,然后加入生物素化的沉默調節(jié)蛋白1(SIRT1)抗體,將未結合的生物素化抗體洗凈后,加入HRP標記的親和素,再次徹底洗滌后加入TMB底物顯色。TMB在過氧化物酶的催化下轉化成藍色,并在酸的作用下轉化成最終的黃色。顏色的深淺和樣品中的沉默調節(jié)蛋白1(SIRT1)呈正相關。用酶標儀在450nm波長下測定吸光度(O.D.值),計算樣品濃度。

相關產品

編號 適用物種:Mus musculus (Mouse,小鼠) 應用(僅供研究使用,不用于臨床診斷!)
RPE912Mu01 沉默調節(jié)蛋白1(SIRT1)重組蛋白 Positive Control; Immunogen; SDS-PAGE; WB.
RPE912Mu02 沉默調節(jié)蛋白1(SIRT1)重組蛋白 Positive Control; Immunogen; SDS-PAGE; WB.
PAE912Mu01 沉默調節(jié)蛋白1(SIRT1)多克隆抗體 WB; IHC; ICC; IP.
PAE912Mu02 沉默調節(jié)蛋白1(SIRT1)多克隆抗體 WB; IHC; ICC; IP.
LAE912Mu71 沉默調節(jié)蛋白1(SIRT1)多克隆抗體(生物素標記) WB; IHC; ICC.
SEE912Mu 沉默調節(jié)蛋白1(SIRT1)檢測試劑盒(酶聯(lián)免疫吸附試驗法) Enzyme-linked immunosorbent assay for Antigen Detection.
LME912Mu 沉默調節(jié)蛋白1(SIRT1)等多因子檢測試劑盒(流式熒光發(fā)光法) FLIA Kit for Antigen Detection.
KSE912Mu01 沉默調節(jié)蛋白1(SIRT1)檢測試劑盒DIY材料(酶聯(lián)免疫吸附試驗法) 自己動手制作ELISA試劑盒的主要材料

參考文獻

雜志 參考文獻
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PLOS ONE A Novel Role for SIRT-1 in L-Arginine Protection against STZ Induced Myocardial Fibrosis in Rats [Pubmed:25501750]
Journal of Alzheimer&#039;s Disease Serum miR-206 and miR-132 as Potential Circulating Biomarkers for Mild Cognitive Impairment [Pubmed:25589731]
Respirology Increases in peripheral SIRT1: A new biological characteristic of asthma [PubMed: 26040995]
PLOS ONE A Remarkable Age-Related Increase in SIRT1 Protein Expression against Oxidative Stress in Elderly: [PubMed: 25785999]
Endocrine Changes of serum Mir-217 and the correlation with the severity in type 2 diabetes patients with different stages of diabetic kidney disease. [pubmed:27522360]
Naunyn Schmiedebergs Arch Pharmacol The potential curative effect of rebamipide in hepatic ischemia/reperfusion injury [pubmed:28361247]
?International journal of cardiology A sirtuin 1/MMP2 prognostic index for myocardial infarction in patients with advanced coronary artery disease [pubmed:28043667]
International Journal of Cardiology Serum concentrations and gene expression of sirtuin 1 in healthy and slightly overweight subjects after caloric restriction or resveratrol supplementation: A randomized trial [pubmed:28029409]
Hepatology International Association of SIRT1 gene polymorphism and its expression for the risk of alcoholic fatty liver disease in the Han population [pubmed:29189974]
Toxicology and Applied Pharmacology Sildenafil protects against bile duct ligation induced hepaticfibrosis in rats: Potential role for silent information regulator 1 (SIRT1) [pubmed:28974454]
Chemico-biological Interactions Magnolol protects pancreatic β-cells against methylglyoxal-induced cellular dysfunction [pubmed:28919305]
Nutrients Gene Expression of Sirtuin-1 and Endogenous Secretory Receptor for Advanced Glycation End Products in Healthy and Slightly Overweight Subjects after?… [Pubmed:30037068]
Experimental Gerontology Specific alterations in the circulating levels of the SIRT1, TLR4, and IL7 proteins in patients with dementia [Pubmed:30071285]
Kidney?and?Blood?Pressure?Research The Kidney Specific Protein myo-Inositol Oxygenase, a Potential Biomarker for Diabetic Nephropathy [Pubmed: 30504713]
Journal of International Medical Research Serum and follicular fluid levels of sirtuin 1, sirtuin 6, and resveratrol in women undergoing fertilization: an observational, clinical study [Pubmed: 30556451]
BioMed Research International Centella asiatica Prevents Increase of Hippocampal Tumor Necrosis Factor-Independently of Its Effect on Brain-Derived Neurotrophic Factor in Rat Model of Chronic?… []
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Biomed?Research International Prevents Increase of Hippocampal Tumor Necrosis Factor-α Independently of Its Effect on Brain-Derived Neurotrophic Factor in Rat Model of?… [Pubmed: 30956976]
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Compr Psychiatry Crocin alleviates schizophrenia-like symptoms in rats by upregulating silent information regulator-1 and brain derived neurotrophic factor [Pubmed: 33045669]
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Journal of Cereal Science Dietary buckwheat enhances sirtuin1 without calorie restriction []
Clin Interv Aging Molecular Changes in Circulating microRNAs' Expression and Oxidative Stress in Adults with Mild Cognitive Impairment: A Biochemical and Molecular Study [33447019]
Antioxidants The Effect of Repeated Whole-Body Cryotherapy on Sirt1 and Sirt3 Concentrations and Oxidative Status in Older and Young Men Performing Different Levels of??- [33396247]
Int Urol Nephrol Evaluation of sirtuin 1 (SIRT1) levels in autosomal dominant polycystic kidney disease [33864594]
Neurochem Int Dimethyl fumarate abridged tauo-/amyloidopathy in a D-Galactose/ovariectomy-induced Alzheimer's-like disease: Modulation of AMPK/SIRT-1, AKT/CREB/BDNF, AKT ?- [34052296]
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Cell & Bioscience OGT Protects Skeletal Muscle From Damage Through SIRT1 O-GlcNAcylation During Cold Stress []
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