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Ⅳ型膠原(COL4)等多因子檢測試劑盒(流式熒光發光法)

Multiplex Assay Kit for Collagen Type IV (COL4) ,etc. by FLIA (Flow Luminescence Immunoassay)

Type-IV Collagen

(注:單次混測多因子不超過8個指標 )

  • Ⅳ型膠原(COL4)等多因子檢測試劑盒(流式熒光發光法) 產品包裝(模擬)
  • Ⅳ型膠原(COL4)等多因子檢測試劑盒(流式熒光發光法) 產品包裝(模擬)
  • Certificate 通過ISO 9001、ISO 13485質量體系認證

特異性

本試劑盒用于檢測Ⅳ型膠原(COL4)等多因子檢測試劑盒(流式熒光發光法),經檢測與其它相似物質無明顯交叉反應。
由于受到技術及樣本來源的限制,不可能完成對所有相關或相似物質交叉反應檢測,因此本試劑盒有可能與未經檢測的其它物質有交叉反應。

回收率

分別于定值血清及血漿樣本中加入一定量的Ⅳ型膠原(COL4)等多因子檢測試劑盒(流式熒光發光法)(加標樣品),重復測定并計算其均值,回收率為測定值與理論值的比率。

樣本 回收率范圍(%) 平均回收率(%)
serum(n=5) 97-104 101
EDTA plasma(n=5) 78-96 88
heparin plasma(n=5) 98-105 101
sodium citrate plasma(n=5) 89-101 96

精密度

精密度用樣品測定值的變異系數CV表示。CV(%) = SD/mean×100
批內差:取同批次試劑盒對低、中、高值定值樣本進行定量檢測,每份樣本連續測定20 次,分別計算不同濃度樣本的平均值及SD值。
批間差:選取3個不同批次的試劑盒分別對低、中、高值定值樣本進行定量測定,每個樣本使用同一試劑盒重復測定8次,分別計算不同濃度樣本的平均值及SD值。
批內差: CV<10%
批間差: CV<12%

線性

在定值血清及血漿樣本內加入適量的Ⅳ型膠原(COL4)等多因子檢測試劑盒(流式熒光發光法),并倍比稀釋成1:2,1:4,1:8,1:16的待測樣本,線性范圍即為稀釋后樣本中Ⅳ型膠原(COL4)等多因子檢測試劑盒(流式熒光發光法)含量的測定值與理論值的比率。

樣本 1:2 1:4 1:8 1:16
serum(n=5) 94-102% 88-104% 89-104% 94-103%
EDTA plasma(n=5) 98-105% 85-97% 78-105% 83-101%
heparin plasma(n=5) 90-102% 78-105% 94-104% 93-103%
sodium citrate plasma(n=5) 82-101% 99-105% 87-101% 78-104%

穩定性

經測定,試劑盒在有效期內按推薦溫度保存,其活性降低率小于5%。
為減小外部因素對試劑盒破壞前后檢測值的影響,實驗室的環境條件需盡量保持一致,尤其是實驗室內溫度、濕度及溫育條件。其次由同一實驗員來進行操作可減少人為誤差。

實驗流程

1. 實驗前標準品、試劑及樣本準備;
2. 加樣(標準品、樣本、磁珠)標準品或樣本100μL及磁珠10μL,
    37°C酶標板振蕩器孵育90分鐘;
3. 磁吸甩干,加檢測溶液A100μL,37°C酶標板振蕩器孵育60分鐘;
4. 磁吸洗板3次;
5. 加檢測溶液B100μL,37°C振動孵育30分鐘;
6. 磁吸洗板3次;
7. 加鞘液100μL,旋渦震蕩2分鐘后讀數。

實驗原理

將Ⅳ型膠原(COL4)等多因子檢測試劑盒(流式熒光發光法)抗體包被于磁珠,制成固相載體,向微孔中分別加入標準品或標本以及磁珠,其中的Ⅳ型膠原(COL4)等多因子檢測試劑盒(流式熒光發光法)與連接于固相載體上的抗體結合,然后加入生物素化的Ⅳ型膠原(COL4)等多因子檢測試劑盒(流式熒光發光法)抗體,將未結合的生物素化抗體洗凈后,加入PE標記的親和素,再次徹底洗滌后即可上機讀數。MFI值和樣品中的Ⅳ型膠原(COL4)等多因子檢測試劑盒(流式熒光發光法)呈正相關。

贈品

相關產品

編號 適用物種:Homo sapiens (Human,人) 應用(僅供研究使用,不用于臨床診斷!)
NPA180Hu01 Ⅳ型膠原(COL4)天然蛋白 Positive Control; Immunogen; SDS-PAGE; WB.
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LMA180Hu Ⅳ型膠原(COL4)等多因子檢測試劑盒(流式熒光發光法) FLIA Kit for Antigen Detection.
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參考文獻

雜志 參考文獻
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