鐵蛋白(FE)等多因子檢測試劑盒(流式熒光發光法)
Multiplex Assay Kit for Ferritin (FE) ,etc. by FLIA (Flow Luminescence Immunoassay)
（注：單次混測多因子不超過8個指標 ）
- 編號LMA518Ga
- 物種Chicken (Gallus，雞) 相同的名稱，不同的物種。
- 實驗方法雙抗夾心
- 反應時長3.5h
- 檢測范圍0.39-400ng/mL
- 靈敏度最小可檢測劑量小于等于0.13 ng/mL.
- 樣本類型Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
- 下載 英文說明書 中文說明書
- 規格 8指標數 7指標數 6指標數 5指標數 4指標數 3指標數 2指標數1指標數
- 價格 ¥ 2562 ¥ 2660 ¥ 2808 ¥ 3005 ¥ 3202 ¥ 3497 ¥ 3941 ¥ 4926 添加到價格計算器 計算器
- 欲了解實際交易價格和更多情況，請與當地經銷商聯系!
-
產品包裝(模擬)
-
產品包裝(模擬)
-
通過ISO 9001、ISO 13485質量體系認證
特異性
本試劑盒用于檢測鐵蛋白(FE)等多因子檢測試劑盒(流式熒光發光法)，經檢測與其它相似物質無明顯交叉反應。
由于受到技術及樣本來源的限制，不可能完成對所有相關或相似物質交叉反應檢測，因此本試劑盒有可能與未經檢測的其它物質有交叉反應。
回收率
分別于定值血清及血漿樣本中加入一定量的鐵蛋白(FE)等多因子檢測試劑盒(流式熒光發光法)（加標樣品），重復測定并計算其均值，回收率為測定值與理論值的比率。
| 樣本 | 回收率范圍(%) | 平均回收率(%) |
| serum(n=5) | 80-102 | 87 |
| EDTA plasma(n=5) | 96-104 | 99 |
| heparin plasma(n=5) | 89-96 | 92 |
精密度
精密度用樣品測定值的變異系數CV表示。CV(%) = SD/mean×100
批內差：取同批次試劑盒對低、中、高值定值樣本進行定量檢測,每份樣本連續測定20 次，分別計算不同濃度樣本的平均值及SD值。
批間差：選取3個不同批次的試劑盒分別對低、中、高值定值樣本進行定量測定，每個樣本使用同一試劑盒重復測定8次，分別計算不同濃度樣本的平均值及SD值。
批內差： CV<10%
批間差： CV<12%
線性
在定值血清及血漿樣本內加入適量的鐵蛋白(FE)等多因子檢測試劑盒(流式熒光發光法)，并倍比稀釋成1:2，1:4，1:8，1:16的待測樣本，線性范圍即為稀釋后樣本中鐵蛋白(FE)等多因子檢測試劑盒(流式熒光發光法)含量的測定值與理論值的比率。
| 樣本 | 1:2 | 1:4 | 1:8 | 1:16 |
| serum(n=5) | 90-97% | 87-101% | 81-89% | 82-91% |
| EDTA plasma(n=5) | 89-97% | 95-103% | 92-99% | 78-94% |
| heparin plasma(n=5) | 97-104% | 80-105% | 96-103% | 92-104% |
穩定性
經測定，試劑盒在有效期內按推薦溫度保存，其活性降低率小于5%。
為減小外部因素對試劑盒破壞前后檢測值的影響，實驗室的環境條件需盡量保持一致，尤其是實驗室內溫度、濕度及溫育條件。其次由同一實驗員來進行操作可減少人為誤差。
實驗流程
1. 實驗前標準品、試劑及樣本準備；
2. 加樣（標準品、樣本、磁珠）標準品或樣本100μL及磁珠10μL，
37°C酶標板振蕩器孵育90分鐘；
3. 磁吸甩干，加檢測溶液A100μL，37°C酶標板振蕩器孵育60分鐘；
4. 磁吸洗板3次；
5. 加檢測溶液B100μL，37°C振動孵育30分鐘；
6. 磁吸洗板3次；
7. 加鞘液100μL，旋渦震蕩2分鐘后讀數。
實驗原理
將鐵蛋白(FE)等多因子檢測試劑盒(流式熒光發光法)抗體包被于磁珠，制成固相載體，向微孔中分別加入標準品或標本以及磁珠，其中的鐵蛋白(FE)等多因子檢測試劑盒(流式熒光發光法)與連接于固相載體上的抗體結合，然后加入生物素化的鐵蛋白(FE)等多因子檢測試劑盒(流式熒光發光法)抗體，將未結合的生物素化抗體洗凈后，加入PE標記的親和素，再次徹底洗滌后即可上機讀數。MFI值和樣品中的鐵蛋白(FE)等多因子檢測試劑盒(流式熒光發光法)呈正相關。
贈品
增值服務
相關產品
| 編號 | 適用物種：Chicken (Gallus，雞) | 應用(僅供研究使用，不用于臨床診斷！) |
| SEA518Ga | 鐵蛋白(FE)檢測試劑盒(酶聯免疫吸附試驗法) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| LMA518Ga | 鐵蛋白(FE)等多因子檢測試劑盒(流式熒光發光法) | FLIA Kit for Antigen Detection. |
參考文獻
| 雜志 | 參考文獻 |
| Molecular Endocrinology | PGC-1α regulates hepatic hepcidin expression and iron homeostasis in response to inflammation. [Pubmed: 23438894] |
| experimental oncology | Redox-regulation of gelatinases during growth of cisplatin-sensitive and resistant Guerin carcinoma. [PubMed: 25804229] |
| J Cancer Sci Ther | Host H68D Genotype Affects Tumor Growth in Mouse Melanoma [Open-Access: 1948-5956-1000353] |
| Food Funct | The effects of polysaccharides from the root of Angelica sinensis on tumor growth and iron metabolism in H22-bearing mice [Pubmed:26757699] |
| Physiological research | The Labile Iron Pool in Monocytes Reflects the Activity of the Atherosclerotic Process in Men with Chronic Cardiovascular Disease [pubmed:27782743] |
| Expert?Review?of Hematology | 4 (th) Mediterranean Multidisciplinary Course on Iron Anemia April 29 (th)-30 (th) 2016, Madrid, Spain. [pubmed:27615544] |
| Oxidative Medicine and Cellular Longevity | Acidic Polysaccharide from Angelica sinensis Reverses Anemia of Chronic Disease Involving the Suppression of Inflammatory Hepcidin and NF-κB Activation [pubmed:29147463] |
| European Journal of Pharmaceutical Sciences | sucrosomial? iron absorption studied by in vitro and ex-vivo models [pubmed:29055735] |
| Journal of Food Science and Technology | In vitro assessment of bio-augmented minerals from peanut oil cakes fermented by Aspergillus oryzae through Caco-2 cells. [pubmed:29051659] |
| International Journal of Cuban Health & Medicine | Anemia and Iron Deficiency Related to Inflammation, Helicobacter pylori Infection and Adiposity in Reproductive-age Cuban Women [10.1590/MEDICC.2017.1902030004?] |
| Oman Medical Journal | The Role of Insulin Therapy in Correcting Hepcidin Levels in Patients with Type 2 Diabetes Mellitus. [pubmed:28584599] |
| Food Bioscience | Preparation of lactose-iron complex and its cyto-toxicity, in-vitro digestion and bioaccessibility in Caco-2 cell model system [10.1016/j.fbio.2017.10.001] |
| Nutrients. | Long-Term Dexamethasone Exposure Down-Regulates Hepatic TFR1 and Reduces Liver Iron Concentration in Rats [pubmed:28629118] |
| Food?Chemistry | Physicochemical characterization of mineral (iron/zinc) bound caseinate and their mineral uptake in Caco-2 cells [Pubmed:29622185] |
| Animal Feed Science and Technology | Super-dosing phytase improves the growth performance of weaner pigs fed a low iron diet [10.1016:j.anifeedsci.2018.01.012] |
| American Journal of Hematology | Iron homeostasis in pregnancy and spontaneous abortion [Pubmed: 30394565] |
| The Nigerian postgraduate medical journal the official publication of the National Postgraduate Medical College of Nigeria | Assessment of iron deficiency anaemia and its risk factors among adults with chronic kidney disease in a tertiary hospital in Nigeria [] |
| Haematologica | New thiazolidinones reduce iron overload in mouse models of hereditary hemochromatosis and β-thalassemia [Pubmed: 30792208] |
| 博士論文 | PHYSIOLOGICAL ADAPTATIONS TO HEAT ACCLIMATION; REPERCUSSIONS ON CYCLING PERFORMANCE [] |
| Synthesis, characterization and cellular mineral absorption of nanoemulsions of Rhododendron arboreum flower extracts stabilized with gum arabic [] | |
| Cell Research | Transferrin plays a central role in coagulation balance by interacting with clotting factors [Pubmed: 31811276] |
| international journal of biological macromolecules | Gum arabic capped copper nanoparticles: Synthesis, characterization, and applications [Pubmed: 31904465] |
| journal of the american college of nutrition | Improvement of Mineral Absorption and Nutritional Properties of Citrullus vulgaris Seeds Using Solid-State Fermentation [Pubmed: 32255407] |
| LWT-FOOD SCIENCE AND TECHNOLOGY | Whey protein-iron or zinc complexation decreases pro-oxidant activity of iron and increases iron and zinc bioavailability [] |
| J Mol Cell Cardiol | Systemic iron deficiency does not affect the cardiac iron content and progression of heart failure [34139233] |
| European Journal of Pharmacology | The mechanism of the imbalance between proliferation and ferroptosis in pulmonary artery smooth muscle cells based on the activation of SLC7A11 [Pubmed:35700835] |
