紅細胞衍生核因子2樣蛋白2(NFE2L2)等多因子檢測試劑盒(流式熒光發光法)
Multiplex Assay Kit for Nuclear Factor, Erythroid Derived 2 Like Protein 2 (NFE2L2) ,etc. by FLIA (Flow Luminescence Immunoassay)
NRF2; HEBP1; NF-E2-Related Factor 2; Nuclear factor, erythroid derived 2, like 2
（注：單次混測多因子不超過8個指標 ）
- 編號LML947Hu
- 物種Homo sapiens (Human，人) 相同的名稱，不同的物種。
- 實驗方法雙抗夾心
- 反應時長3.5h
- 檢測范圍0.01-10ng/mL
- 靈敏度最小可檢測劑量小于等于0.003 ng/mL.
- 樣本類型Tissue homogenates, cell lysates and other biological fluids.
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產品包裝(模擬)
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產品包裝(模擬)
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通過ISO 9001、ISO 13485質量體系認證
特異性
本試劑盒用于檢測紅細胞衍生核因子2樣蛋白2(NFE2L2)等多因子檢測試劑盒(流式熒光發光法)，經檢測與其它相似物質無明顯交叉反應。
由于受到技術及樣本來源的限制，不可能完成對所有相關或相似物質交叉反應檢測，因此本試劑盒有可能與未經檢測的其它物質有交叉反應。
回收率
分別于定值血清及血漿樣本中加入一定量的紅細胞衍生核因子2樣蛋白2(NFE2L2)等多因子檢測試劑盒(流式熒光發光法)（加標樣品），重復測定并計算其均值，回收率為測定值與理論值的比率。
| 樣本 | 回收率范圍(%) | 平均回收率(%) |
| serum(n=5) | 79-91 | 84 |
| EDTA plasma(n=5) | 78-89 | 82 |
| heparin plasma(n=5) | 90-97 | 93 |
| sodium citrate plasma(n=5) | 85-103 | 89 |
精密度
精密度用樣品測定值的變異系數CV表示。CV(%) = SD/mean×100
批內差：取同批次試劑盒對低、中、高值定值樣本進行定量檢測,每份樣本連續測定20 次，分別計算不同濃度樣本的平均值及SD值。
批間差：選取3個不同批次的試劑盒分別對低、中、高值定值樣本進行定量測定，每個樣本使用同一試劑盒重復測定8次，分別計算不同濃度樣本的平均值及SD值。
批內差： CV<10%
批間差： CV<12%
線性
在定值血清及血漿樣本內加入適量的紅細胞衍生核因子2樣蛋白2(NFE2L2)等多因子檢測試劑盒(流式熒光發光法)，并倍比稀釋成1:2，1:4，1:8，1:16的待測樣本，線性范圍即為稀釋后樣本中紅細胞衍生核因子2樣蛋白2(NFE2L2)等多因子檢測試劑盒(流式熒光發光法)含量的測定值與理論值的比率。
| 樣本 | 1:2 | 1:4 | 1:8 | 1:16 |
| serum(n=5) | 87-98% | 78-101% | 94-105% | 95-103% |
| EDTA plasma(n=5) | 90-104% | 96-104% | 93-101% | 94-103% |
| heparin plasma(n=5) | 79-93% | 92-105% | 90-101% | 85-94% |
| sodium citrate plasma(n=5) | 79-91% | 82-93% | 88-96% | 93-101% |
穩定性
經測定，試劑盒在有效期內按推薦溫度保存，其活性降低率小于5%。
為減小外部因素對試劑盒破壞前后檢測值的影響，實驗室的環境條件需盡量保持一致，尤其是實驗室內溫度、濕度及溫育條件。其次由同一實驗員來進行操作可減少人為誤差。
實驗流程
1. 實驗前標準品、試劑及樣本準備；
2. 加樣（標準品、樣本、磁珠）標準品或樣本100μL及磁珠10μL，
37°C酶標板振蕩器孵育90分鐘；
3. 磁吸甩干，加檢測溶液A100μL，37°C酶標板振蕩器孵育60分鐘；
4. 磁吸洗板3次；
5. 加檢測溶液B100μL，37°C振動孵育30分鐘；
6. 磁吸洗板3次；
7. 加鞘液100μL，旋渦震蕩2分鐘后讀數。
實驗原理
將紅細胞衍生核因子2樣蛋白2(NFE2L2)等多因子檢測試劑盒(流式熒光發光法)抗體包被于磁珠，制成固相載體，向微孔中分別加入標準品或標本以及磁珠，其中的紅細胞衍生核因子2樣蛋白2(NFE2L2)等多因子檢測試劑盒(流式熒光發光法)與連接于固相載體上的抗體結合，然后加入生物素化的紅細胞衍生核因子2樣蛋白2(NFE2L2)等多因子檢測試劑盒(流式熒光發光法)抗體，將未結合的生物素化抗體洗凈后，加入PE標記的親和素，再次徹底洗滌后即可上機讀數。MFI值和樣品中的紅細胞衍生核因子2樣蛋白2(NFE2L2)等多因子檢測試劑盒(流式熒光發光法)呈正相關。
贈品
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相關產品
| 編號 | 適用物種：Homo sapiens (Human，人) | 應用(僅供研究使用，不用于臨床診斷！) |
| RPL947Hu01 | 紅細胞衍生核因子2樣蛋白2(NFE2L2)重組蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| PAL947Hu01 | 紅細胞衍生核因子2樣蛋白2(NFE2L2)多克隆抗體 | WB; IHC; ICC; IP. |
| MAL947Hu21 | 紅細胞衍生核因子2樣蛋白2(NFE2L2)單克隆抗體 | WB; IHC; ICC; IP. |
| SEL947Hu | 紅細胞衍生核因子2樣蛋白2(NFE2L2)檢測試劑盒(酶聯免疫吸附試驗法) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| LML947Hu | 紅細胞衍生核因子2樣蛋白2(NFE2L2)等多因子檢測試劑盒(流式熒光發光法) | FLIA Kit for Antigen Detection. |
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