降鈣素原(PCT)等多因子檢測試劑盒(流式熒光發光法)
Multiplex Assay Kit for Procalcitonin (PCT) ,etc. by FLIA (Flow Luminescence Immunoassay)
Pro-Calcitonin
（注：單次混測多因子不超過8個指標 ）
- 編號LMA689Hu
- 物種Homo sapiens (Human，人) 相同的名稱，不同的物種。
- 實驗方法雙抗夾心
- 反應時長3.5h
- 檢測范圍1.95-2000pg/mL
- 靈敏度最小可檢測劑量小于等于0.65 pg/mL.
- 樣本類型Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
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產品包裝(模擬)
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產品包裝(模擬)
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通過ISO 9001、ISO 13485質量體系認證
特異性
本試劑盒用于檢測降鈣素原(PCT)等多因子檢測試劑盒(流式熒光發光法)，經檢測與其它相似物質無明顯交叉反應。
由于受到技術及樣本來源的限制，不可能完成對所有相關或相似物質交叉反應檢測，因此本試劑盒有可能與未經檢測的其它物質有交叉反應。
回收率
分別于定值血清及血漿樣本中加入一定量的降鈣素原(PCT)等多因子檢測試劑盒(流式熒光發光法)（加標樣品），重復測定并計算其均值，回收率為測定值與理論值的比率。
| 樣本 | 回收率范圍(%) | 平均回收率(%) |
| serum(n=5) | 92-101 | 96 |
| EDTA plasma(n=5) | 98-105 | 101 |
| heparin plasma(n=5) | 86-104 | 94 |
精密度
精密度用樣品測定值的變異系數CV表示。CV(%) = SD/mean×100
批內差：取同批次試劑盒對低、中、高值定值樣本進行定量檢測,每份樣本連續測定20 次，分別計算不同濃度樣本的平均值及SD值。
批間差：選取3個不同批次的試劑盒分別對低、中、高值定值樣本進行定量測定，每個樣本使用同一試劑盒重復測定8次，分別計算不同濃度樣本的平均值及SD值。
批內差： CV<10%
批間差： CV<12%
線性
在定值血清及血漿樣本內加入適量的降鈣素原(PCT)等多因子檢測試劑盒(流式熒光發光法)，并倍比稀釋成1:2，1:4，1:8，1:16的待測樣本，線性范圍即為稀釋后樣本中降鈣素原(PCT)等多因子檢測試劑盒(流式熒光發光法)含量的測定值與理論值的比率。
| 樣本 | 1:2 | 1:4 | 1:8 | 1:16 |
| serum(n=5) | 84-91% | 92-101% | 89-104% | 90-98% |
| EDTA plasma(n=5) | 92-101% | 79-96% | 88-104% | 95-102% |
| heparin plasma(n=5) | 98-105% | 83-91% | 96-105% | 95-102% |
穩定性
經測定，試劑盒在有效期內按推薦溫度保存，其活性降低率小于5%。
為減小外部因素對試劑盒破壞前后檢測值的影響，實驗室的環境條件需盡量保持一致，尤其是實驗室內溫度、濕度及溫育條件。其次由同一實驗員來進行操作可減少人為誤差。
實驗流程
1. 實驗前標準品、試劑及樣本準備；
2. 加樣（標準品、樣本、磁珠）標準品或樣本100μL及磁珠10μL，
37°C酶標板振蕩器孵育90分鐘；
3. 磁吸甩干，加檢測溶液A100μL，37°C酶標板振蕩器孵育60分鐘；
4. 磁吸洗板3次；
5. 加檢測溶液B100μL，37°C振動孵育30分鐘；
6. 磁吸洗板3次；
7. 加鞘液100μL，旋渦震蕩2分鐘后讀數。
實驗原理
將降鈣素原(PCT)等多因子檢測試劑盒(流式熒光發光法)抗體包被于磁珠，制成固相載體，向微孔中分別加入標準品或標本以及磁珠，其中的降鈣素原(PCT)等多因子檢測試劑盒(流式熒光發光法)與連接于固相載體上的抗體結合，然后加入生物素化的降鈣素原(PCT)等多因子檢測試劑盒(流式熒光發光法)抗體，將未結合的生物素化抗體洗凈后，加入PE標記的親和素，再次徹底洗滌后即可上機讀數。MFI值和樣品中的降鈣素原(PCT)等多因子檢測試劑盒(流式熒光發光法)呈正相關。
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相關產品
| 編號 | 適用物種：Homo sapiens (Human，人) | 應用(僅供研究使用，不用于臨床診斷！) |
| RPA689Hu02 | 降鈣素原(PCT)重組蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| RPA689Hu01 | 降鈣素原(PCT)重組蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| RPA689Hu03 | 降鈣素原(PCT)重組蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| CPA689Hu21 | 降鈣素原(PCT)卵白蛋白偶聯物 | Immunogen; SDS-PAGE; WB. |
| PAA689Hu01 | 降鈣素原(PCT)多克隆抗體 | WB; IHC; ICC; IP. |
| PAA689Hu02 | 降鈣素原(PCT)多克隆抗體 | WB; IHC; ICC; IP. |
| PAA689Hu08 | 降鈣素原(PCT)多克隆抗體 | WB; IHC; ICC; IP. |
| LAA689Hu71 | 降鈣素原(PCT)多克隆抗體(生物素標記) | WB; IHC; ICC. |
| MAA689Hu28 | 降鈣素原(PCT)單克隆抗體 | WB; IHC; ICC; IP. |
| MAA689Hu21 | 降鈣素原(PCT)單克隆抗體 | WB; IHC; ICC; IP. |
| MAA689Hu29 | 降鈣素原(PCT)單克隆抗體 | WB; IHC; ICC; IP. |
| MAA689Hu22 | 降鈣素原(PCT)單克隆抗體 | WB; IHC; ICC; IP. |
| MAA689Hu23 | 降鈣素原(PCT)單克隆抗體 | WB; IHC; ICC; IP. |
| MAA689Hu27 | 降鈣素原(PCT)單克隆抗體 | WB; IHC; ICC; IP. |
| MAA689Hu24 | 降鈣素原(PCT)單克隆抗體 | WB; IHC; ICC; IP. |
| MAA689Hu25 | 降鈣素原(PCT)單克隆抗體 | WB; IHC; ICC; IP. |
| SEA689Hu | 降鈣素原(PCT)檢測試劑盒(酶聯免疫吸附試驗法) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| SCA689Hu | 降鈣素原(PCT)檢測試劑盒(化學發光免疫分析法) | Chemiluminescent immunoassay for Antigen Detection. |
| LMA689Hu | 降鈣素原(PCT)等多因子檢測試劑盒(流式熒光發光法) | FLIA Kit for Antigen Detection. |
| PSA689Hu01 | 降鈣素原(PCT)抗體對 | ELISA; CLIA; ELISPOT; Luminex; Immunochromatography and other Immunoassays. |
| KSA689Hu01 | 降鈣素原(PCT)檢測試劑盒DIY材料(酶聯免疫吸附試驗法) | Main materials for "Do It (ELISA Kit) Yourself". |
參考文獻
| 雜志 | 參考文獻 |
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| International Journal of Radiation Oncology ? Biology ? Physics | Effects of Berberine Against Radiation-Induced Intestinal Injury in Mice [PubMed: 20637981] |
| Ulus Travma Acil Cerrahi Derg | Diagnostic and prognostic value of procalcitonin and phosphorus in acute mesenteric ischemia [PubMed: 21935794] |
| Egyptian Journal of Bronchology | Serum procalcitonin and its relation to the outcome of ventilator-associated pneumoniain children [Essbronchology: Source] |
| University of Waikato | The Assessment of Vitamin D, Antimicrobial Peptides and Procalcitonin in Bronchiectasis [Waikato:Source] |
| Egyptian Journal of Medical Microbiology | Evaluation of the Diagnostic Utility of Fc Gamma Receptor Type 1 (nCD64) and Procalcitonin in Comparison with Conventional Methods for Diagnosis of Early Onset Neonatal Sepsis [Ejmm:Source] |
| Nigerian Medical Journal | Efficacy of measuring procalcitonin levels in determination of prognosis and early diagnosis of bacterial resistance in sepsis [Pubmed:25657488] |
| Wiley online library | The use and value of procalcitonin in solid organ transplantation [PubMed: 25996831] |
| Balkan Med J | Diagnostic Value of Procalcitonin Levels in Acute Mesenteric Ischemia [PubMed: 26185718] |
| The Journal of Clinical Endocrinology & Metabolism | Macrocalcitonin is a novel pitfall in the routine of serum calcitonin immunoassay [PubMed: 26647152] |
| Journal of Human Hypertension | Procalcitonin and Pentraxin-3: Current biomarkers in inflammation in white coat hypertension [PubMed: 26040439] |
| Medical Science Monitor | Variation?of?Circulating?Inflammatory?Mediators?in?Staphylococcus?aureus?and?Escherichia?coli?BloodstreamInfection. [Pubmed:26772168] |
| International Immunopharmacology | Consistency and pathophysiological characterization of a rat polymicrobial sepsis model via the improved cecal ligation and puncture surgery [Pubmed:26802602] |
| Pancreatology. | Early prediction of intestinal mucosal barrier function impairment by elevated serum procalcitonin in rats with severe acute pancreatitis. [Pubmed:26804005] |
| Acta?Medica | Risk Factors?of?Acute?Pancreatitis?in?Oral?Double Balloon Enteroscopy. [pubmed:27638962] |
| The Clinical Respiratory Journal | Changes in inflammatory mediators as a result of intermittent hypoxia in obstructive sleep apnea syndrome [pubmed:28960823] |
| SHOCK | INTERLEUKIN-1 RECEPTOR 2: A NEW BIOMARKER FOR SEPSIS DIAGNOSIS AND GRAM-NEGATIVE/GRAM-POSITIVE BACTERIAL DIFFERENTIATION [pubmed:27984536] |
| Cellular?Physiology and Biochemistry | Hepatic Macrophages are the Cell Source of Hepatic Procalcitonin in Acute Liver Failure [Pubmed:29913443] |
| Archives of Medical Science | Inflammatory mediators in the diagnosis and treatment of acute pancreatitis: pentraxin-3, procalcitonin and myeloperoxidase [Pubmed:29593801] |
| Journal of Thoracic Disease | Expression pattern of soluble triggering receptor expressed on myeloid cells-1 in mice with Acinetobacter baumannii colonization and infection in the lung [Pubmed:29707313] |
| Asian?Journal of Psychiatry | Procalcitonin and C-reactive protein as peripheral inflammatory markers in antipsychotic drug-free schizophrenia patients [Pubmed:29723719] |
| European Review for Medical and Pharmacological Sciences | The predictive value of joint detection of serum amyloid protein A, PCT, and Hs-CRP in the diagnosis and efficacy of neonatal septicemia. [Pubmed: 31298341] |
| Medicina | Modestly Elevated Serum Procalcitonin Levels in Patients with Rheumatoid Arthritis Free of Active Infection [Pubmed: 33080909] |
| MEDIATORS OF INFLAMMATION | Reevaluation of Lung Injury in TNF-Induced Shock: The Role of the Acid Sphingomyelinase [Pubmed: 32410851] |
| Early label-free analysis of mitochondrial redox states by Raman spectroscopy predicts septic outcomes [] | |
| Journal of Immunology Research | Moesin Is a Novel Biomarker of Endothelial Injury in Sepsis [33628853] |
| Journal of Medical Biochemistry | Circulating nuclear factor-kappa B mediates cancer-associated inflammation in human breast and colon cancer [33776564] |
| Surg Endosc | Original contribution: sleeve gastrectomy reduces soluble lectin-like oxidized low-density lipoprotein receptor-1 (sLOX-1) levels in patients with morbid obesity [Pubmed:35044516] |
| Bone Res | Procalcitonin is expressed in osteoblasts and limits bone resorption through inhibition of macrophage migration during intermittent PTH treatment [Pubmed:35087025] |
