S100鈣結合蛋白A8(S100A8)等多因子檢測試劑盒(流式熒光發光法)
Multiplex Assay Kit for S100 Calcium Binding Protein A8 (S100A8) ,etc. by FLIA (Flow Luminescence Immunoassay)
MRP8; MIF; 60B8AG; CAGA; CFAG; CGLA; CP-10; L1Ag; NIF; P8; Calgranulin A; Cystic fibrosis antigen; Leukocyte L1 complex light chain; Migration inhibitory factor-related protein 8
（注：單次混測多因子不超過8個指標 ）
- 編號LMB792Hu
- 物種Homo sapiens (Human，人) 相同的名稱，不同的物種。
- 實驗方法雙抗夾心
- 反應時長3.5h
- 檢測范圍0.98-1000pg/mL
- 靈敏度最小可檢測劑量小于等于0.327 pg/mL.
- 樣本類型serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
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產品包裝(模擬)
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產品包裝(模擬)
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通過ISO 9001、ISO 13485質量體系認證
特異性
本試劑盒用于檢測S100鈣結合蛋白A8(S100A8)等多因子檢測試劑盒(流式熒光發光法)，經檢測與其它相似物質無明顯交叉反應。
由于受到技術及樣本來源的限制，不可能完成對所有相關或相似物質交叉反應檢測，因此本試劑盒有可能與未經檢測的其它物質有交叉反應。
回收率
分別于定值血清及血漿樣本中加入一定量的S100鈣結合蛋白A8(S100A8)等多因子檢測試劑盒(流式熒光發光法)（加標樣品），重復測定并計算其均值，回收率為測定值與理論值的比率。
| 樣本 | 回收率范圍(%) | 平均回收率(%) |
| serum(n=5) | 97-104 | 101 |
| EDTA plasma(n=5) | 79-91 | 85 |
| heparin plasma(n=5) | 88-95 | 92 |
| sodium citrate plasma(n=5) | 80-102 | 89 |
精密度
精密度用樣品測定值的變異系數CV表示。CV(%) = SD/mean×100
批內差：取同批次試劑盒對低、中、高值定值樣本進行定量檢測,每份樣本連續測定20 次，分別計算不同濃度樣本的平均值及SD值。
批間差：選取3個不同批次的試劑盒分別對低、中、高值定值樣本進行定量測定，每個樣本使用同一試劑盒重復測定8次，分別計算不同濃度樣本的平均值及SD值。
批內差： CV<10%
批間差： CV<12%
線性
在定值血清及血漿樣本內加入適量的S100鈣結合蛋白A8(S100A8)等多因子檢測試劑盒(流式熒光發光法)，并倍比稀釋成1:2，1:4，1:8，1:16的待測樣本，線性范圍即為稀釋后樣本中S100鈣結合蛋白A8(S100A8)等多因子檢測試劑盒(流式熒光發光法)含量的測定值與理論值的比率。
| 樣本 | 1:2 | 1:4 | 1:8 | 1:16 |
| serum(n=5) | 94-102% | 83-104% | 98-105% | 87-95% |
| EDTA plasma(n=5) | 82-101% | 84-99% | 82-105% | 80-90% |
| heparin plasma(n=5) | 95-102% | 97-105% | 97-104% | 86-97% |
| sodium citrate plasma(n=5) | 96-105% | 78-88% | 85-94% | 87-96% |
穩定性
經測定，試劑盒在有效期內按推薦溫度保存，其活性降低率小于5%。
為減小外部因素對試劑盒破壞前后檢測值的影響，實驗室的環境條件需盡量保持一致，尤其是實驗室內溫度、濕度及溫育條件。其次由同一實驗員來進行操作可減少人為誤差。
實驗流程
1. 實驗前標準品、試劑及樣本準備；
2. 加樣（標準品、樣本、磁珠）標準品或樣本100μL及磁珠10μL，
37°C酶標板振蕩器孵育90分鐘；
3. 磁吸甩干，加檢測溶液A100μL，37°C酶標板振蕩器孵育60分鐘；
4. 磁吸洗板3次；
5. 加檢測溶液B100μL，37°C振動孵育30分鐘；
6. 磁吸洗板3次；
7. 加鞘液100μL，旋渦震蕩2分鐘后讀數。
實驗原理
將S100鈣結合蛋白A8(S100A8)等多因子檢測試劑盒(流式熒光發光法)抗體包被于磁珠，制成固相載體，向微孔中分別加入標準品或標本以及磁珠，其中的S100鈣結合蛋白A8(S100A8)等多因子檢測試劑盒(流式熒光發光法)與連接于固相載體上的抗體結合，然后加入生物素化的S100鈣結合蛋白A8(S100A8)等多因子檢測試劑盒(流式熒光發光法)抗體，將未結合的生物素化抗體洗凈后，加入PE標記的親和素，再次徹底洗滌后即可上機讀數。MFI值和樣品中的S100鈣結合蛋白A8(S100A8)等多因子檢測試劑盒(流式熒光發光法)呈正相關。
贈品
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相關產品
| 編號 | 適用物種：Homo sapiens (Human，人) | 應用(僅供研究使用，不用于臨床診斷！) |
| APB792Hu01 | S100鈣結合蛋白A8(S100A8)活性蛋白 | Cell?culture;?Activity?Assays. |
| RPB792Hu02 | S100鈣結合蛋白A8(S100A8)重組蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| RPB792Hu01 | S100鈣結合蛋白A8(S100A8)重組蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| PAB792Hu01 | S100鈣結合蛋白A8(S100A8)多克隆抗體 | WB; IHC; ICC; IP. |
| PAB792Hu02 | S100鈣結合蛋白A8(S100A8)多克隆抗體 | WB; IHC; ICC; IP; FCM |
| LAB792Hu81 | S100鈣結合蛋白A8(S100A8)多克隆抗體(異硫氰酸熒光素標記) | WB; IHC; ICC; IF. |
| LAB792Hu71 | S100鈣結合蛋白A8(S100A8)多克隆抗體(生物素標記) | WB; IHC; ICC. |
| MAB792Hu21 | S100鈣結合蛋白A8(S100A8)單克隆抗體 | WB; IHC. |
| MAB792Hu25 | S100鈣結合蛋白A8(S100A8)單克隆抗體 | WB; IHC; ICC; IP. |
| MAB792Hu22 | S100鈣結合蛋白A8(S100A8)單克隆抗體 | WB; IHC; ICC; IP. |
| MAB792Hu24 | S100鈣結合蛋白A8(S100A8)單克隆抗體 | WB; ICC/IF |
| MAB792Hu27 | S100鈣結合蛋白A8(S100A8)單克隆抗體 | WB; IHC; ICC; IP. |
| MAB792Hu29 | S100鈣結合蛋白A8(S100A8)單克隆抗體 | WB; IHC; ICC; IP. |
| MAB792Hu26 | S100鈣結合蛋白A8(S100A8)單克隆抗體 | WB; IHC; ICC; IP. |
| MAB792Hu28 | S100鈣結合蛋白A8(S100A8)單克隆抗體 | WB; IHC; ICC; IP. |
| MAB792Hu23 | S100鈣結合蛋白A8(S100A8)單克隆抗體 | WB; IHC; ICC; IP. |
| FAB792Hu02 | 抗S100鈣結合蛋白A8(S100A8)單克隆抗體 | Flow cytometry. |
| FAB792Hu04 | 抗S100鈣結合蛋白A8(S100A8)單克隆抗體 | Flow cytometry. |
| FAB792Hu06 | 抗S100鈣結合蛋白A8(S100A8)單克隆抗體 | Flow cytometry. |
| SEB792Hu | S100鈣結合蛋白A8(S100A8)檢測試劑盒(酶聯免疫吸附試驗法) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| LMB792Hu | S100鈣結合蛋白A8(S100A8)等多因子檢測試劑盒(流式熒光發光法) | FLIA Kit for Antigen Detection. |
| PSB792Hu01 | S100鈣結合蛋白A8(S100A8)抗體對 | ELISA; CLIA; ELISPOT; Luminex; Immunochromatography and other Immunoassays. |
| KSB792Hu01 | S100鈣結合蛋白A8(S100A8)檢測試劑盒DIY材料(酶聯免疫吸附試驗法) | Main materials for "Do It (ELISA Kit) Yourself". |
參考文獻
| 雜志 | 參考文獻 |
| Biochemical and Biophysical Research Communications | High circulating levels of S100A8/A9 complex (calprotectin) in male Japanese with abdominal adiposity and dysregulated expression of S100A8 and S100A9 in adipose tissues of obese mice [ScienceDirect: S0006291X1200352X] |
| American Journal of Reproductive Immunology | Association of Increased S100A8 Serum Protein with Early Pregnancy Loss [Pubmed:25252120] |
| the faseb journal | Soluble receptor for advanced glycation end products inhibits disease progression in autosomal dominant polycystic kidney disease by down-regulating cell proliferation [PubMed: 25934702] |
| J Immunol | S100A8 Production in CXCR2-Expressing CD11b+ Gr-1high Cells Aggravates Hepatitis in Mice Fed a High-Fat and High-Cholesterol Diet [PubMed: 26608915] |
| PLoS?One | Analytical Performance of ELISA Assays in Urine: One More Bottleneck towards Biomarker Validation and Clinical Implementation [Pubmed:26889680] |
| Journal?of?Alzheimer's?Disease | Proteomics?Analysis?of?Blood?Serums?from?Alzheimer's?Disease?Patients?Using?iTRAQ?LabelingTechnology. [pubmed:27911324] |
| ?Journal of Functional Foods | Standardized ethanolic extract of the rhizome of Curcuma xanthorrhiza prevents murine ulcerative colitis by regulation of inflammation [S1756464617300208] |
| International?Journal?of?Molecular?Medicine | Proinflammatory effects of S100A8/A9 via TLR4 and RAGE signaling pathways in BV-2 microglial cells [pubmed:28498464] |
| International journal of molecular sciences | Immune Modulating Topical S100A8/A9 Inhibits Growth of Pseudomonas aeruginosa and Mitigates Biofilm Infection in Chronic Wounds. [pubmed:28672877] |
| Gastroenterology | Tu1938-Micronutrients Assessment in Patients with Cirrhosis [10.1016:S0016-5085(18)33544-3] |
| Gastroenterology | Tu1942-PI3K/AKT-Mediated Upregulation of C7 Inhibits Colorectal Cancer Proliferation and Metastasis [10.1016:S0016-5085(18)33544-5] |
| Journal of Biological Chemistry | Signaling between pancreatic β-cells and macrophages via S100 calcium-binding protein A8 exacerbates β-cell apoptosis and islet inflammation [Pubmed:29496993] |
| European?review for?medical?and pharmacological?sciences | S100A8/A9 promotes MMP-9 expression in the fibroblasts from cardiac rupture after myocardial infarction by inducing macrophages secreting TNFα. [Pubmed:29949169] |
| Foodborne Pathogens and Disease | Synergistic effect of immunomodulatory S100A8/A9 and ciprofloxacin against Pseudomonas aeruginosa biofilm in a murine chronic wound model [Pubmed: 31116394] |
| Kidney International Reports | Systemic Inflammation Precedes Microalbuminuria in Diabetes [] |
| Cancer Management and Research | iTRAQ-based quantitative protein expression profiling of biomarkers in childhood B-cell and T-cell acute lymphoblastic leukemia [Pubmed: 31440093] |
| scandinavian journal of clinical & laboratory investigation | Serum S100A6, S100A8, S100A9 and S100A11 proteins in colorectal neoplasia: results of a single centre prospective study [Pubmed: 31856598] |
| ANNALS OF AGRICULTURAL AND ENVIRONMENTAL MEDICINE | Analysis of novel serum markers of fibrosis and angiogenesis in patients with alcoholic liver cirrhosis [] |
| MOLECULAR VISION | Quantification of a panel for dry-eye protein biomarkers in tears: A comparative pilot study using standard ELISA and customized microarrays [34012227] |
| university of copenhagen | Adjunctive S100A8/A9 Immunomodulation Hinders Cipro fl oxacin Resistance in [] |
