腦啡肽酶(CD10)重組蛋白
Recombinant Neprilysin (CD10)
NEP; MME; CALLA; SFE; Atriopeptidase; Enkephalinase; Skin fibroblast elastase; Common Acute Lymphoblastic Leukemia Antigen; Membrane Metallo-Endopeptidase
- 編號RPB785Hu02
- 物種Homo sapiens (Human，人) 相同的名稱，不同的物種。
- 來源原核表達
- 宿主E.coli
- 內毒素水平<1.0EU/µg(LAL法測定)
- 亞細胞定位細胞膜
- 預測分子量22.3kDa
- 實際分子量22kDa(差異分析請參閱說明書)
- 片段與標簽Phe565~Trp750 with N-terminal His Tag
- 緩沖液成份20mM Tris, 150mM NaCl緩沖液（pH8.0, 含有1mM EDTA, 1mM DTT, 0.01% SKL, 5% Trehalose和Proclin300）
- 性狀凍干粉
- 純度> 97%
- 等電點6.6
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應用
Positive Control; Immunogen; SDS-PAGE; WB.
如果需要有生物活性的蛋白，請參見活性蛋白。 - 下載 英文說明書 中文說明書
- 規格 10μg50μg 200μg 1mg 5mg
- 價格 ¥ 1200 ¥ 3000 ¥ 6000 ¥ 18000 ¥ 45000
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產品包裝(模擬)
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產品包裝(模擬)
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Figure . Gene Sequencing (extract)
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SDS-PAGE圖
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通過ISO 9001、ISO 13485質量體系認證
序列
用法
Reconstitute in 20mM Tris, 150mM NaCl (pH8.0) to a concentration of 0.1-1.0 mg/mL. Do not vortex.
儲存
避免反復凍融。2-8°C不超過一個月，-80°C不超過12個月。
穩定性
熱穩定性以損失率顯示。損失率是由加速降解試驗決定，具體方法如下：在37°C孵育48小時，沒有顯著的降解或者沉淀產生。保質期內，在適當的條件下存儲，損失率低于5%。
增值服務
相關產品
| 編號 | 適用物種：Homo sapiens (Human，人) | 應用(僅供研究使用，不用于臨床診斷！) |
| APB785Hu61 | 腦啡肽酶(CD10)活性蛋白 | Cell?culture;?Activity?Assays. |
| RPB785Hu02 | 腦啡肽酶(CD10)重組蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| RPB785Hu01 | 腦啡肽酶(CD10)重組蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| EPB785Hu61 | 腦啡肽酶(CD10)真核蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| PAB785Hu01 | 腦啡肽酶(CD10)多克隆抗體 | WB; IHC; ICC; IP. |
| PAB785Hu02 | 腦啡肽酶(CD10)多克隆抗體 | WB; IHC; ICC; IP. |
| LAB785Hu71 | 腦啡肽酶(CD10)多克隆抗體(生物素標記) | WB; IHC; ICC. |
| MAB785Hu21 | 腦啡肽酶(CD10)單克隆抗體 | WB; IHC; ICC; IP. |
| MAB785Hu23 | 腦啡肽酶(CD10)單克隆抗體 | WB; IHC; ICC; IP. |
| MAB785Hu24 | 腦啡肽酶(CD10)單克隆抗體 | WB; IHC; ICC; IP. |
| MAB785Hu22 | 腦啡肽酶(CD10)單克隆抗體 | WB; IHC; ICC; IP. |
| MAB785Hu25 | 腦啡肽酶(CD10)單克隆抗體 | WB; IHC; ICC; IP. |
| MAB785Hu27 | 腦啡肽酶(CD10)單克隆抗體 | WB; IHC; ICC; IP. |
| MAB785Hu29 | 腦啡肽酶(CD10)單克隆抗體 | WB; IHC; ICC; IP. |
| MAB785Hu28 | 腦啡肽酶(CD10)單克隆抗體 | WB; IHC; ICC; IP. |
| MAB785Hu26 | 腦啡肽酶(CD10)單克隆抗體 | WB; IHC; ICC; IP. |
| FAB785Hu02 | 抗腦啡肽酶(CD10)單克隆抗體 | FCM |
| FAB785Hu04 | 抗腦啡肽酶(CD10)單克隆抗體 | FCM |
| FAB785Hu06 | 抗腦啡肽酶(CD10)單克隆抗體 | FCM |
| FAB785Hu08 | 抗腦啡肽酶(CD10)單克隆抗體 | FCM |
| FAB785Hu00 | 抗腦啡肽酶(CD10)單克隆抗體 | Flow cytometry. |
| FAB785Hu82 | 抗腦啡肽酶(CD10)單克隆抗體(異硫氰酸熒光素標記) | FCM |
| SEB785Hu | 腦啡肽酶(CD10)檢測試劑盒(酶聯免疫吸附試驗法) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| LMB785Hu | 腦啡肽酶(CD10)等多因子檢測試劑盒(流式熒光發光法) | FLIA Kit for Antigen Detection. |
參考文獻
| 雜志 | 參考文獻 |
| 13 | Elevated Plasma levels of Neutral Endpeptidase in Hypertension Patients Treated with Angiotensin Converting Enzyme Inhibitors [Ahajournals:Source] |
| Biol Pharm Bull | Effect of High Glucose Levels on Amyloid β Production in Retinas of Spontaneous Diabetes Mellitus Otsuka Long-Evans Tokushima Fatty Rats [PubMed: 25832640] |
| Eur J Heart Fail.? | Soluble neprilysin does not correlate with outcome in heart failure with preserved ejection fraction [Pubmed:26725876] |
| 8 | A?Test?in?Context:?Neprilysin:?Function,?Inhibition, and?Biomarker. [pubmed:27491909] |
| European?Journal of?Heart?Failure | Effects of sacubitril/valsartan on neprilysin targets and the metabolism of natriuretic peptides in chronic heart failure: a mechanistic clinical study [Pubmed: 30520545] |
| European?Journal of?Heart?Failure | Increased granulocyte membrane neprilysin (CD10) expression is associated with better prognosis in heart failure [Pubmed: 30828920] |
| Clinical Research in Cardiology | Soluble neprilysin, NT-proBNP, and growth differentiation factor-15 as biomarkers for heart failure in dialysis patients (SONGBIRD) [Pubmed: 32002632] |
| Neurotoxicology | Involvement of hippocampal agmatine in β1-42 amyloid induced memory impairment, neuroinflammation and BDNF signaling disruption in mice [Pubmed: 32522471] |
| European Heart Journal | P1620 Association of granulocyte neprilysin (CD10) expression with prognosis in heart failure with reduced ejection fraction [] |
| Clin Biochem | Finding a reliable assay for soluble neprilysin [Pubmed:35331754] |
| Scientific Reports | The potential role of serum tau protein (MAPT), neuronal cell adhesion molecule (NrCAM) and neprilysin (NEP) in neurodegenerative disorders development in?… [] |
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