分泌型卷曲相關蛋白1(SFRP1)重組蛋白
Recombinant Secreted Frizzled Related Protein 1 (SFRP1)
FRP1; FRP; FRP-1; FrzA; SARP2; Secreted apoptosis-related protein 2
- 編號RPF880Mu01
- 物種Mus musculus (Mouse，小鼠) 相同的名稱，不同的物種。
- 來源原核表達
- 宿主E.coli
- 內毒素水平<1.0EU/µg(LAL法測定)
- 亞細胞定位分泌
- 預測分子量34.2kDa
- 實際分子量35kDa(差異分析請參閱說明書)
- 片段與標簽Ser32~Lys314 with N-terminal His Tag
- 緩沖液成份磷酸鹽緩沖液（pH7.4，含有 0.01% SKL, 1mM DTT, 5% Trehalose和Proclin300.）
- 性狀凍干粉
- 純度> 95%
- 等電點9.0
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應用
Positive Control; Immunogen; SDS-PAGE; WB.
如果需要有生物活性的蛋白，請參見活性蛋白。 - 下載 英文說明書 中文說明書
- 規格 10μg50μg 200μg 1mg 5mg
- 價格 ¥ 1008 ¥ 2520 ¥ 5040 ¥ 15120 ¥ 37800
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產品包裝(模擬)
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產品包裝(模擬)
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Figure. Gene Sequencing (Extract)
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Figure. SDS-PAGE
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通過ISO 9001、ISO 13485質量體系認證
序列
用法
Reconstitute in 10mM PBS (pH7.4) to a concentration of 0.1-1.0 mg/mL. Do not vortex.
儲存
避免反復凍融。2-8°C不超過一個月，-80°C不超過12個月。
穩定性
熱穩定性以損失率顯示。損失率是由加速降解試驗決定，具體方法如下：在37°C孵育48小時，沒有顯著的降解或者沉淀產生。保質期內，在適當的條件下存儲，損失率低于5%。
增值服務
相關產品
| 編號 | 適用物種：Mus musculus (Mouse，小鼠) | 應用(僅供研究使用，不用于臨床診斷！) |
| RPF880Mu02 | 分泌型卷曲相關蛋白1(SFRP1)重組蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| RPF880Mu01 | 分泌型卷曲相關蛋白1(SFRP1)重組蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| PAF880Mu01 | 分泌型卷曲相關蛋白1(SFRP1)多克隆抗體 | WB; IHC; ICC; IP. |
| PAF880Mu02 | 分泌型卷曲相關蛋白1(SFRP1)多克隆抗體 | WB; IHC; ICC; IP. |
| LAF880Mu71 | 分泌型卷曲相關蛋白1(SFRP1)多克隆抗體(生物素標記) | WB; IHC; ICC. |
| LAF880Mu81 | 分泌型卷曲相關蛋白1(SFRP1)多克隆抗體(異硫氰酸熒光素標記) | WB; IHC; ICC; IF. |
| SEF880Mu | 分泌型卷曲相關蛋白1(SFRP1)檢測試劑盒(酶聯免疫吸附試驗法) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| LMF880Mu | 分泌型卷曲相關蛋白1(SFRP1)等多因子檢測試劑盒(流式熒光發光法) | FLIA Kit for Antigen Detection. |
| KSF880Mu01 | 分泌型卷曲相關蛋白1(SFRP1)檢測試劑盒DIY材料(酶聯免疫吸附試驗法) | 自己動手制作ELISA試劑盒的主要材料 |
參考文獻
| 雜志 | 參考文獻 |
| BMC Musculoskeletal Disorders | Biomarkers and cytokines of bone turnover: extensive evaluation in a cohort of patients with ankylosing spondylitis [PubMed: 23025387] |
| Exp Cell Res | Wnt Inhibition is Dysregulated in Gliomas and its Re-Establishment Inhibits Proliferation and Tumor Sphere Formation [PubMed: 26712519] |
| BMC Musculoskeletal Disorders | Correlation?of?blood?bone?turnover?biomarkers?and?Wnt?signaling?antagonists?with AS,?DISH,OPLL, and?OYL. [pubmed:28153008] |
| PloS one | Effects of exercise training on circulating levels of Dickkpof-1 and secreted frizzled-related protein-1 in breast cancer survivors: A pilot single-blind randomized controlled trial. [pubmed:28178355] |
| PLoS One | Effects of exercise training on circulating levels of Dickkpof-1 and secreted frizzled-related protein-1 in breast cancer survivors: A pilot single-blind randomized controlled trial [10.1371] |
| Journal of Experimental & Clinical Cancer Research | PHF21B overexpression promotes cancer stem cell-like traits in prostate cancer cells by activating the Wnt/β-catenin signaling pathway [pubmed:28645312] |
| JBMR Plus | Low serum levels of DKK2 predict incident low‐impact fracture in older women [Doi: 10.1002/jbm4.10179] |
| Nature Communications | Immunological history governs human stem cell memory CD4 heterogeneity via the Wnt signaling pathway [Pubmed: 32041953] |
| acta pharmacologica sinica | sFRP1 protects H9c2 cardiac myoblasts from doxorubicin-induced apoptosis by inhibiting the Wnt/PCP-JNK pathway [Pubmed: 32238888] |
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